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dc.contributor.authorLópez-Reyes, Alberto
dc.contributor.authorMedina-Luna, Daniel
dc.contributor.authorSantamaría-Olmedo, Mónica
dc.contributor.authorMartínez-Flores, Karina
dc.contributor.authorZamudio-Cuevas, Yessica
dc.contributor.authorFernández-Torres, Javier
dc.contributor.authorMartínez-Nava, Gabriela Angélica
dc.contributor.authorOlivos-Meza, Anell
dc.contributor.authorCamacho-Rea, Carmen
dc.contributor.authorFernández-Moreno, Mercedes
dc.contributor.authorBlanco García, Francisco J
dc.contributor.authorPineda, Carlos
dc.date.accessioned2022-12-07T09:22:59Z
dc.date.available2022-12-07T09:22:59Z
dc.date.issued2021-03-16
dc.identifier.citationLópez-Reyes A, Medina-Luna D, Santamaría-Olmedo M, Martínez-Flores K, Zamudio-Cuevas Y, Fernández-Torres J, Martínez-Nava GA, Olivos-Meza A, Camacho-Rea C, Fernández-Moreno M, Blanco FJ, Pineda C. Soluble inflammatory mediators of synoviocytes stimulated by monosodium urate crystals induce the production of oxidative stress, pain, and inflammation mediators in chondrocytes : Secretome of synoviocytes induces chondrocyte damage. Clin Rheumatol. 2021 Aug;40(8):3265-3271.es_ES
dc.identifier.issn0770-3198
dc.identifier.urihttp://hdl.handle.net/2183/32162
dc.descriptionBrief reportes_ES
dc.description.abstract[Abstract] We hypothesized that the secretion of inflammatory mediators from synoviocytes affects the chondrocyte homeostasis of articular cartilage. This study was a preliminary attempt to elucidate the molecular mechanisms by which soluble mediators obtained from activated synoviocytes induce oxidative stress and inflammation in chondrocytes. We measured the concentrations of interleukin-6 (IL-6), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), nerve growth factor (NGF), superoxide anion (O2•-), hydrogen peroxide (H2O2), and nitric oxide (NO•) from articular human cells. First, we created a conditional basal medium by exposing synoviocytes (HS) to monosodium urate crystals (CBM). The chondrocytes were exposed to either CBM (CCM), urate crystals directly (CMSU), or remained untreated (CC) as a negative control. Data were analyzed by ANOVA tests; Bonferroni test was performed for multiple comparisons between groups. Interestingly, we observed that mediators of inflammation and oxidative stress were significantly higher in CCM than CMSU and CC groups (P<0.01). The specific concentrations were as follows: 19.85 ng/mL of IL-6, 9.79 ng/mL of IL-8, 5.17 ng/mL of NGF, and 11.91 ng/mL of MCP-1. Of note, we observed the same trend for reactive oxygen and nitrogen species (P<0.001). Soluble mediators secreted by synoviocytes after being activated with MSU crystals (as observed in individuals who present gout attacks) trigger chondrocyte activation intensifying the articular inflammatory, oxidative, and pain states that damage cartilage in OA; this damage is more severe even when compared to HC directly exposed to monosodium urate crystals. Key Points • The molecular relation between MSU depositions and cartilage damage could be mediated by pro-inflammatory soluble mediators and oxidative molecules. • The secretion of pro-inflammatory mediators by activated synoviocytes is more harmful to chondrocytes than a direct activation in the chondrocyte culture. • Under this model, there is an important imbalance in the matrix homeostasis due to changes in several chemokines, cytokines, and other factors such as NGF, as well as oxidative mediators.es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.urihttps://doi.org/10.1007/s10067-021-05676-wes_ES
dc.rightsThis version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at Springer Link web.es_ES
dc.subjectGoutes_ES
dc.subjectOsteoarthritises_ES
dc.subjectOxidative mediatorses_ES
dc.subjectPro-inflammatory Interleukinses_ES
dc.subjectSecretome-activated synoviocyteses_ES
dc.titleSoluble inflammatory mediators of synoviocytes stimulated by monosodium urate crystals induce the production of oxidative stress, pain, and inflammation mediators in chondrocyteses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleClinical Rheumatologyes_ES
UDC.volume40es_ES
UDC.issue8es_ES
UDC.startPage3265es_ES
UDC.endPage3271es_ES


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