Now showing items 1-20 of 184

    • Mitochondrial activity is modulated by TNFα and IL-1β in normal human chondrocyte cells 

      López-Armada, María José; Caramés, Beatriz; Martín, M.A.; Cillero-Pastor, B.; Lires-Dean, M.; Fuentes Boquete, Isaac Manuel; Arenas, J.; Blanco García, Francisco J (Elsevier, 2006-05-05)
      [Abstract] Objective. Pro-inflammatory cytokines play an important role in osteoarthritis (OA). In osteoarthritic cartilage, chondrocytes exhibit an alteration in mitochondrial activity. This study analyzes the effect of ...
    • Tratamiento de lesiones del cartílago articular con terapia celular 

      Fuentes Boquete, Isaac Manuel; Arufe, M.C.; Díaz-Prado, Silvia; Hermida Gómez, Tamara; De-Toro, Javier; Blanco García, Francisco J (Elsevier, 2008-11-13)
      [Resumen] Las lesiones del cartílago articular que no afectan a la integridad del hueso subcondral no se reparan espontáneamente. El carácter asintomático de estas lesiones propicia la progresiva degeneración articular y ...
    • Differentiation of Synovial CD-105+ Human Mesenchymal Stem Cells into Chondrocyte-like Cells through Spheroid Formation 

      Arufe, M.C.; Fuente, Alexandre de la; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Wiley, 2009-06-18)
      [Abstract] Mesenchymal stem cells (MSCs) have the capacity to differentiate into several cell lineages, some of which can generate bone, cartilage, or adipose tissue. The presence of MSCs in the synovial membrane was ...
    • Molecular profile and cellular characterization of human bone marrow mesenchymal stem cells: donor influence on chondrogenesis 

      Cicione, Claudia; Díaz-Prado, Silvia; Muiños-López, Emma; Hermida Gómez, Tamara; Blanco García, Francisco J (Elsevier, 2010)
      [Abstract] Background. The use of autologous or allogenic stem cells has recently been suggested as an alternative therapeutic approach for treatment of cartilage defects. Bone marrow mesenchymal stem cells (BM-MSCs) are ...
    • Potential use of the human amniotic membrane as a scaffold in human articular cartilage repair 

      Díaz-Prado, Silvia; Rendal Vázquez, María Esther; Muiños-López, Emma; Hermida Gómez, Tamara; Rodríguez-Cabarcos, Margarita; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Springer, 2010-04-13)
      [Abstract] The human amniotic membrane (HAM) is an abundant and readily obtained tissue that may be an important source of scaffold for transplanted chondrocytes in cartilage regeneration in vivo. To evaluate the potential ...
    • Chondrogenic Potential of Subpopulations of Cells Expressing Mesenchymal Stem Cell Markers Derived from Human Synovial Membranes 

      Arufe, M.C.; Fuente, Alexandre de la; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Wiley, 2010-07-21)
      [Abstract] In this study we analyzed the chondrogenic potential of subpopulations of mesenchymal stem cells (MSCs) derived from human synovial membranes enriched for CD73, CD106, and CD271 markers. Subpopulations of human ...
    • Multilineage differentiation potential of cells isolated from the human amniotic membrane 

      Díaz-Prado, Silvia; Muiños-López, Emma; Hermida Gómez, Tamara; Rendal Vázquez, María Esther; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Wiley, 2010-11-01)
      [Abstract] The human amniotic membrane (HAM) contains two cell types from different embryological origins. Human amnion epithelial cells (hAECs) are derived from the embryonic ectoderm, while human amnion mesenchymal stromal ...
    • Quantification of cells expressing mesenchymal stem cell markers in healthy and osteoarthritic synovial membranes 

      Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; Gimeno-Longas, María José; Muiños-López, Emma; Díaz-Prado, Silvia; De-Toro, Javier; Blanco García, Francisco J (Journal of Rheumatology Publishing Company, 2010-11-15)
      [Abstract] Objective. To quantify cells expressing mesenchymal stem cell (MSC) markers in synovial mem- branes from human osteoarthritic (OA) and healthy joints. Methods. Synovial membranes from OA and healthy joints were ...
    • Isolation and characterization of mesenchymal stem cells from human amniotic membrane 

      Díaz-Prado, Silvia; Muíños-López, Emma; Hermida Gómez, Tamara; Rendal Vázquez, María Esther; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Mary Ann Liebert, 2011-01-04)
      [Abstract] Introduction: The human amniotic membrane is a highly abundant and readily available tissue that may be useful for regenerative medicine and cell therapy. Aim: To compare two previously published protocols for ...
    • Human amniotic membrane as an alternative source of stem cells for regenerative medicine 

      Díaz-Prado, Silvia; Muiños-López, Emma; Hermida Gómez, Tamara; Cicione, Claudia; Rendal Vázquez, María Esther; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Elsevier, 2011-02-19)
      [Abstract] The human amniotic membrane (HAM) is a highly abundant and readily available tissue. This amniotic tissue has considerable advantageous characteristics to be considered as an attractive material in the field of ...
    • Bone marrow cells immunomagnetically selected for CD271+ antigen promote in vitro the repair of articular cartilage defects 

      Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; Gimeno-Longas, María José; Muíños-López, Emma; Díaz-Prado, Silvia; De-Toro, Javier; Blanco García, Francisco J (Mary Ann Liebert, 2011-03-22)
      [Abstract] Objective: The purposes of this project were to quantify the cells expressing the mesenchymal stem cell (MSC) marker CD271 in synovial membranes from human osteoarthritic (OA) and healthy joints, and to determine ...
    • A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues 

      Rodríguez-Rigueiro, Teresa; Valladares-Ayerbes, Manuel; Haz, Mar; Blanco, Moisés; Aparicio Gallego, Guadalupe; Fernández-Puente, Patricia; Blanco García, Francisco J; Lorenzo-Patiño, María J.; Antón-Aparicio, Luis M.; Figueroa, Angélica (Wiley, 2011-05-18)
      [Abstract] Most of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry ...
    • Umbilical Cord as a Mesenchymal Stem Cell Source for Treating Joint Pathologies 

      Arufe, M.C.; Fuente, Alexandre de la; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Baishideng, 2011-06-18)
      [Abstract] Articular cartilage disorders and injuries often result in life-long chronic pain and compromised quality of life. Regrettably, the regeneration of articular cartilage is a continuing challenge for biomedical ...
    • Analysis of the Chondrogenic Potential and Secretome of Mesenchymal Stem Cells Derived from Human Umbilical Cord Stroma 

      Arufe, M.C.; Fuente, Alexandre de la; Mateos, Jesús; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J (Mary Ann Liebert, 2011-06-28)
      [Abstract] Mesenchymal stem cells (MSCs) from umbilical cord stroma were isolated by plastic adherence and characterized by flow cytometry, looking for cells positive for OCT3/4 and SSEA-4 as well as the classic MSC markers ...
    • Proteome Analysis During Chondrocyte Differentiation in a New Chondrogenesis Model Using Human Umbilical Cord Stroma Mesenchymal Stem Cells 

      Fuente, Alexandre de la; Mateos, Jesús; Lesende-Rodríguez, Iván; Calamia, Valentina; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Arufe, M.C.; Blanco García, Francisco J (American Society for Biochemistry and Molecular Biology, 2011-10-17)
      [Abstract] Umbilical cord stroma mesenchymal stem cells were differentiated toward chondrocyte-like cells using a new in vitro model that consists of the random formation of spheroids in a medium supplemented with fetal ...
    • Mitochondrial DNA (mtDNA) haplogroups and serum levels of anti-oxidant enzymes in patients with osteoarthritis 

      Fernández-Moreno, Mercedes; Soto-Hermida, Ángel; Pértega-Díaz, Sonia; Oreiro, Natividad; Fernández-López, Carlos; Rego-Pérez, Ignacio; Blanco García, Francisco J (BioMed Central, 2011-11-22)
      [Abstract] Background: Oxidative stress play a main role in the initiation and progression of the OA disease and leads to the degeneration of mitochondria. To prevent this, the chondrocytes possess a well-coordinated ...
    • Cryopreservation effect on proliferative and chondrogenic potential of human chondrocytes isolated from superficial and deep cartilage 

      Muíños López, Emma; Rendal Vázquez, María Esther; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia; Blanco García, Francisco J (Bentham Open, 2012-04-06)
      [Abstract] Objectives: To compare the proliferative and chondrogenic potential of fresh and frozen chondrocytes isolated from superficial and deep articular cartilage biopsies. Materials and Methodology: The study ...
    • Mitochondrial haplogroups define two phenotypes of osteoarthritis 

      Fernández-Moreno, Mercedes; Soto-Hermida, Ángel; Oreiro, Natividad; Pértega-Díaz, Sonia; Fernández-López, Carlos; Rego-Pérez, Ignacio; Blanco García, Francisco J (Frontiers, 2012-05-11)
      [Abstract] Objective: To assess a mitochondrion-related phenotype in patients with osteoarthritis (OA). Methods: Serum levels of the following OA-related biomarkers: matrix metalloproteinase-1 (MMP-1); MMP-3; MMP-13; ...
    • Characterization of microRNA expression profiles in normal and osteoarthritic human chondrocytes 

      Díaz-Prado, Silvia; Cicione, Claudia; Muiños-López, Emma; Hermida Gómez, Tamara; Oreiro, Natividad; Fernández-López, Carlos; Blanco García, Francisco J (BioMed Central, 2012-08-12)
      [Abstract] Background. Osteoarthritis (OA) is a multifactorial disease characterized by destruction of the articular cartilage due to environmental, mechanical and genetic components. The genetics of OA is complex and ...
    • Mitochondrial haplogroups H and J: risk and protective factors for ischemic cardiomyopathy 

      Fernández Caggiano, Mariana; Barallobre-Barreiro, Javier; Rego-Pérez, Ignacio; Crespo-Leiro, María Generosa; Paniagua-Martín, María J.; Grille Cancela, Zulaika; Blanco García, Francisco J; Doménech, Nieves (PLoS, 2012-08-28)
      [Abstract] Background. Since mitochondria are the principal source of reactive oxygen species (ROS), these organelles may play an important role in ischemic cardiomyopathy (IC) development. The mitochondrial genome may ...