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Niosomes-Based Gene Delivery Systems for Effective Transfection of Human Mesenchymal Stem Cells
dc.contributor.author | Carballo-Pedrares, Natalia | |
dc.contributor.author | Kattar, Axel | |
dc.contributor.author | Concheiro, Ángel | |
dc.contributor.author | Álvarez-Lorenzo, Carmen | |
dc.contributor.author | Rey-Rico, Ana | |
dc.date.accessioned | 2021-10-25T10:23:26Z | |
dc.date.available | 2021-10-25T10:23:26Z | |
dc.date.issued | 2021-07-11 | |
dc.identifier.citation | Natalia Carballo-Pedrares, Axel Kattar, Angel Concheiro, Carmen Alvarez-Lorenzo, Ana Rey-Rico, Niosomes-based gene delivery systems for effective transfection of human mesenchymal stem cells, Materials Science and Engineering: C, Volume 128, 2021, 112307, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2021.112307. (https://www.sciencedirect.com/science/article/pii/S092849312100446X) | |
dc.identifier.issn | 0928-4931 | |
dc.identifier.uri | http://hdl.handle.net/2183/28713 | |
dc.description | Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG | es_ES |
dc.description.abstract | [Abstract] Gene transfer to mesenchymal stem cells (MSCs) has arisen as a powerful approach to increase the therapeutic potential of this effective cell population. Over recent years, niosomes have emerged as self-assembled carriers with promising performance for gene delivery. The aim of our work was to develop effective niosomes-based DNA delivery platforms for targeting MSCs. Niosomes based on 1,2-di-O-octadecenyl-3-trimethylammonium propane (DOTMA; 0, 7 or 15%) as cationic lipid, cholesterol as helper lipid, and polysorbate 60 as non-ionic surfactant, were prepared using a reverse phase evaporation technique. Niosomes dispersions (filtered or not) and their corresponding nioplexes with a lacZ plasmid were characterized in terms of size, charge, protection, and complexation abilities. DOTMA concentration had a large influence on the physicochemical properties of resulting nioplexes. Transfection efficiency and cytotoxic profiles were assessed in two immortalized cell lines of MSCs. Niosomes formulated with 15% DOTMA provided the highest values of β-galactosidase activity, being similar to those achieved with Lipofectamine®, but showed less cytotoxicity. Filtration of niosomes dispersions before adding to the cells resulted in a loss of their biological activities. Storage of niosomes formulations (for 30 days at room temperature) caused minor modification of their physicochemical properties but also attenuated the transfection capability of the nioplexes. Differently, addition of the lysosomotropic agent sucrose into the culture medium during transfection or to the formulation itself improved the transfection performance of non-filtered niosomes. Indeed, steam heat-sterilized niosomes prepared in sucrose medium demonstrated transfection capability. | es_ES |
dc.description.sponsorship | The work was supported by MICINN [RTI2018-099389-A-100; PID2020-113881RB-I00], Agencia Estatal de Investigación (AEI) Spain, Xunta de Galicia [ED431C 2020/17], and FEDER. A. Rey-Rico acknowledges MICINN for the Ramón y Cajal Fellowship [RYC2018-025617-I] and Universidade da Coruña/CISUG for the funding for open access charge. This project was also funded by the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Actions grant agreement N° 813440 (ORBITAL–Ocular Research by Integrated Training And Learning) | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED431C 2020/17 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099389-A-I00/ES/CRIOGELES ACTIVADOS POR GENES PARA REPARACION DE CARTILAGO | |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113881RB-I00/ES/ | |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC2018-025617-I/ES/ | |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/813440 | |
dc.relation.uri | https://doi.org/10.1016/j.msec.2021.112307 | es_ES |
dc.rights | Atribución 4.0 Internacional | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | DOTMA | es_ES |
dc.subject | Gene transfer | es_ES |
dc.subject | MSCs | es_ES |
dc.subject | Niosomes | es_ES |
dc.subject | Stability | es_ES |
dc.subject | Sucrose | es_ES |
dc.title | Niosomes-Based Gene Delivery Systems for Effective Transfection of Human Mesenchymal Stem Cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Materials Science and Engineering: C | es_ES |
UDC.volume | 128 | es_ES |
UDC.startPage | 112307 | es_ES |
dc.identifier.doi | 10.1016/j.msec.2021.112307 |
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