Mostrar o rexistro simple do ítem
Amentadione from the Alga Cystoseira usneoides as a Novel Osteoarthritis Protective Agent in an Ex Vivo Co-Culture OA Model
dc.contributor.author | Araújo, Nuna | |
dc.contributor.author | Viegas, Carla S.B. | |
dc.contributor.author | Zubía, Eva | |
dc.contributor.author | Magalhaes, Joana | |
dc.contributor.author | Ramos, Acácio | |
dc.contributor.author | Carvalho, Maria M. | |
dc.contributor.author | Cruz, Henrique | |
dc.contributor.author | Sousa, Joao Paulo | |
dc.contributor.author | Blanco García, Francisco J | |
dc.contributor.author | Vermeer, Cees | |
dc.contributor.author | Simes, Dina C. | |
dc.date.accessioned | 2021-01-13T08:51:01Z | |
dc.date.available | 2021-01-13T08:51:01Z | |
dc.date.issued | 2020-12-07 | |
dc.identifier.citation | Araújo N, Viegas CSB, Zubía E, Magalhaes J, Ramos A, Carvalho MM, et al. Amentadione from the alga "cystoseira usneoides" as a novel osteoarthritis protective agent in an ex vivo co-culture OA model. Mar Drugs. 2020; 18(12):624 | es_ES |
dc.identifier.issn | 1660-3397 | |
dc.identifier.uri | http://hdl.handle.net/2183/27098 | |
dc.description.abstract | [Abstract] Osteoarthritis (OA) remains a prevalent chronic disease without effective prevention and treatment. Amentadione (YP), a meroditerpenoid purified from the alga Cystoseira usneoides, has demonstrated anti-inflammatory activity. Here, we investigated the YP anti-osteoarthritic potential, by using a novel OA preclinical drug development pipeline designed to evaluate the anti-inflammatory and anti-mineralizing activities of potential OA-protective compounds. The workflow was based on in vitro primary cell cultures followed by human cartilage explants assays and a new OA co-culture model, combining cartilage explants with synoviocytes under interleukin-1β (IL-1β) or hydroxyapatite (HAP) stimulation. A combination of gene expression analysis and measurement of inflammatory mediators showed that the proposed model mimicked early disease stages, while YP counteracted inflammatory responses by downregulation of COX-2 and IL-6, improved cartilage homeostasis by downregulation of MMP3 and the chondrocytes hypertrophic differentiation factors Col10 and Runx2. Importantly, YP downregulated NF-κB gene expression and decreased phosphorylated IkBα/total IkBα ratio in chondrocytes. These results indicate the co-culture as a relevant pre-clinical OA model, and strongly suggest YP as a cartilage protective factor by inhibiting inflammatory, mineralizing, catabolic and differentiation processes during OA development, through inhibition of NF-κB signaling pathways, with high therapeutic potential. | es_ES |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (Portugal); DL57/2016/CP1361/CT0006 | es_ES |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (Portugal); UIDB/04326/2020. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation.uri | https://doi.org/10.3390/md18120624 | es_ES |
dc.rights | Atribución 4.0 España | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/es/ | * |
dc.subject | Osteoarthritis | es_ES |
dc.subject | Amentadione | es_ES |
dc.subject | Preclinical osteoarthritis models | es_ES |
dc.subject | Marine compounds | es_ES |
dc.subject | Cystoseira usneoides | es_ES |
dc.subject | Inflammation | es_ES |
dc.subject | Mineralization | es_ES |
dc.subject | Chondrocytes | es_ES |
dc.subject | Synoviocytes | es_ES |
dc.subject | Cartilage explants | es_ES |
dc.title | Amentadione from the Alga Cystoseira usneoides as a Novel Osteoarthritis Protective Agent in an Ex Vivo Co-Culture OA Model | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Marine Drugs | es_ES |
UDC.volume | 18 | es_ES |
UDC.issue | 12 | es_ES |
UDC.startPage | 624 | es_ES |
Ficheiros no ítem
Este ítem aparece na(s) seguinte(s) colección(s)
-
INIBIC- REUMA - Artigos [184]