dc.contributor.author | Rodríguez-Pereira, Cristina | |
dc.contributor.author | Lagunas, Anna | |
dc.contributor.author | Casanellas, Ignasi | |
dc.contributor.author | Vida, Yolanda | |
dc.contributor.author | Pérez-Inestrosa, Ezequiel | |
dc.contributor.author | Andrades, José A. | |
dc.contributor.author | Becerra, José | |
dc.contributor.author | Samitier, Josep | |
dc.contributor.author | Blanco García, Francisco J | |
dc.contributor.author | Magalhaes, Joana | |
dc.date.accessioned | 2020-06-05T08:20:53Z | |
dc.date.available | 2020-06-05T08:20:53Z | |
dc.date.issued | 2020-05-13 | |
dc.identifier.citation | Rodríguez-Pereira, C.; Lagunas, A.; Casanellas, I.; Vida, Y.; Pérez-Inestrosa, E.; Andrades, J.A.; Becerra, J.; Samitier, J.; Blanco, F.J.; Magalhães, J. RGD-Dendrimer-Poly(L-lactic) Acid Nanopatterned Substrates for the Early Chondrogenesis of Human Mesenchymal Stromal Cells Derived from Osteoarthritic and Healthy Donors. Materials 2020, 13, 2247. https://doi.org/10.3390/ma13102247 | es_ES |
dc.identifier.issn | 1996-1944 | |
dc.identifier.uri | http://hdl.handle.net/2183/25684 | |
dc.description.abstract | [Abstract] Aiming to address a stable chondrogenesis derived from mesenchymal stromal cells (MSCs) to be applied in cartilage repair strategies at the onset of osteoarthritis (OA), we analyzed the effect of arginine–glycine–aspartate (RGD) density on cell condensation that occurs during the initial phase of chondrogenesis. For this, we seeded MSC-derived from OA and healthy (H) donors in RGD-dendrimer-poly(L-lactic) acid (PLLA) nanopatterned substrates (RGD concentrations of 4 × 10−9, 10−8, 2.5 × 10−8, and 10−2 w/w), during three days and compared to a cell pellet conventional three-dimensional culture system. Molecular gene expression (collagens type-I and II–COL1A1 and COL2A1, tenascin-TNC, sex determining region Y-box9-SOX9, and gap junction protein alpha 1–GJA1) was determined as well as the cell aggregates and pellet size, collagen type-II and connexin 43 proteins synthesis. This study showed that RGD-tailored first generation dendrimer (RGD-Cys-D1) PLLA nanopatterned substrates supported the formation of pre-chondrogenic condensates from OA- and H-derived human bone marrow-MSCs with enhanced chondrogenesis regarding the cell pellet conventional system (presence of collagen type-II and connexin 43, both at the gene and protein level). A RGD-density dependent trend was observed for aggregates size, in concordance with previous studies. Moreover, the nanopatterns’ had a higher effect on OA-derived MSC morphology, leading to the formation of bigger and more compact aggregates with improved expression of early chondrogenic markers. | es_ES |
dc.description.sponsorship | This research was funded by the CIBER-BBN Intramural Projects Program (CHONDRONANONET). CIBER-BBN is a national initiative of ISCIII. The authors further acknowledge funds from the INTERREG V cooperation program for Spain–Portugal (POCTEP) 2014–2020 project (0245_IBEROS_1_E), MINECO (CTQ2016-75870-P), Junta de Andalucía (UMA18-FEDERJA-007), CERCA Program, and by the Commission for Universities and Research of the Department of Innovation, Universities, and Enterprise of the Generalitat de Catalunya (2017 SGR 1079). This work was developed in the context of AdvanceCat with the support of ACCIÓ 17 (Catalonia Trade and Investment; Generalitat de Catalunya) under the Catalonian ERDF operational program 2014–2020. C.R.P. acknowledges support from the i-PFIS Doctoral Program (IFI15/00151) funded by ISCIII. I.C. acknowledges support from MINECO through the Subsidies for Predoctoral Contracts for the Training of Doctors open call, co-funded by the European Social Fund (2016) | es_ES |
dc.description.sponsorship | Junta de Andalucía; UMA18-FEDERJA-007 | es_ES |
dc.description.sponsorship | Generalitat de Catalunya; 2017 SGR 1079 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2016-75870-P/ES/NUEVOS DENDRIMEROS Y MARCADORES FLUORESCENTES PARA APLICACIONES BIOMEDICAS/ | |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/IFI15%2F00151/ES/ | |
dc.relation.uri | https://doi.org/10.3390/ma13102247 | es_ES |
dc.rights | Atribución 4.0 Internacional | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Cell condensation | es_ES |
dc.subject | Gap junctions | es_ES |
dc.subject | RGD-density | es_ES |
dc.subject | Chondrogenic differentiation | es_ES |
dc.subject | Osteoarthritis | es_ES |
dc.title | RGD-Dendrimer-Poly(L-lactic) Acid Nanopatterned Substrates for the Early Chondrogenesis of Human Mesenchymal Stromal Cells Derived from Osteoarthritic and Healthy Donors | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Materials | es_ES |
UDC.volume | 13 | es_ES |
UDC.issue | 10 | es_ES |
UDC.startPage | E2247 | es_ES |
dc.identifier.doi | 10.3390/ma13102247 | |