Impact of prevalence ratios of chondroitin sulfate (CS)- 4 and -6 isomers derived from marine sources in cell proliferation and chondrogenic differentiation processes
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Impact of prevalence ratios of chondroitin sulfate (CS)- 4 and -6 isomers derived from marine sources in cell proliferation and chondrogenic differentiation processesAutor(es)
Data
2020-01-31Cita bibliográfica
López-Senra E, Casal-Beiroa P, López-Álvarez M, et al. Impact of prevalence ratios of chondroitin sulfate (CS)- 4 and -6 isomers derived from marine sources in cell proliferation and chondrogenic differentiation processes. Mar Drugs. 2020; 18(2): 94-108
Resumo
[Abstract]
Osteoarthritis is the most prevalent rheumatic disease. During disease progression,
differences have been described in the prevalence of chondroitin sulfate (CS) isomers. Marine
derived-CS present a higher proportion of the 6S isomer, offering therapeutic potential.
Accordingly, we evaluated the effect of exogenous supplementation of CS, derived from the small
spotted catshark (Scyliorhinus canicula), blue shark (Prionace glauca), thornback skate (Raja clavata)
and bovine CS (reference), on the proliferation of osteochondral cell lines (MG-63 and T/C-28a2) and
the chondrogenic differentiation of mesenchymal stromal cells (MSCs). MG-G3 proliferation was
comparable between R. clavata (CS-6 intermediate ratio) and bovine CS (CS-4 enrichment), for
concentrations below 0.5 mg/mL, defined as a toxicity threshold. T/C-28a2 proliferation was
significantly improved by intermediate ratios of CS-6 and -4 isomers (S. canicula and R. clavata). A
dose-dependent response was observed for S. canicula (200 µg/mL vs 50 and 10 µg/mL) and bovine
CS (200 and 100 µg/mL vs 10 µg/mL). CS sulfation patterns discretely affected MSCs
chondrogenesis; even though S. canicula and R. clavata CS up-regulated chondrogenic markers
expression (aggrecan and collagen type II) these were not statistically significant. We demonstrate
that intermediate values of CS-4 and -6 isomers improve cell proliferation and offer potential for
chondrogenic promotion, although more studies are needed to elucidate its mechanism of action.
Palabras chave
Chondroitin sulfate
Osteoarthritis
Chondrogenesis
Fishery by-products
Marine compounds
Circular economy
Osteoarthritis
Chondrogenesis
Fishery by-products
Marine compounds
Circular economy
Versión do editor
Dereitos
Atribución 3.0 España
ISSN
16603397