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Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells
dc.contributor.author | Sanjurjo-Rodríguez, Clara | |
dc.contributor.author | Castro Viñuelas, Rocío | |
dc.contributor.author | Hermida Gómez, Tamara | |
dc.contributor.author | Fuentes Boquete, Isaac Manuel | |
dc.contributor.author | De-Toro, Javier | |
dc.contributor.author | Blanco García, Francisco J | |
dc.contributor.author | Díaz-Prado, Silvia | |
dc.date.accessioned | 2017-11-17T13:11:07Z | |
dc.date.available | 2017-11-17T13:11:07Z | |
dc.date.issued | 2017-09-28 | |
dc.identifier.citation | Sanjurjo-Rodríguez C, Castro-Viñuelas R, Hermida-Gómez T, Fuentes-Boquete IM, de Toro FJ, Blanco FJ, Díaz-Prado SM. Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells. Int J Med Sci. 2017 Sep 28;14(12):1257-1262. | es_ES |
dc.identifier.issn | 1449-1907 | |
dc.identifier.uri | http://hdl.handle.net/2183/19766 | |
dc.description.abstract | [Abstract] The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made in human cartilage biopsies. Injured samples were pre-treated with interleukin 1β (IL1β) for 24 h; also, samples were not pre-treated. hBMSCs were seeded on different types of collagen scaffolds. The resulting constructs were placed into the lesions, and the biopsies were cultured for 2 months in chondrogenic medium. Using the modified ICRSII scale, neotissues from the different scaffolds showed ICRS II overall assessment scores ranging from 50% (fibrocartilage) to 100% (hyaline cartilage), except for the Col I +Col II +HS constructs (fibrocartilage/hyaline cartilage, 73%). Data showed that hBMSCs cultured only on Col I +Col II +HS scaffolds displayed a chondrocyte-like morphology and cartilage-like matrix close to native cartilage. Furthermore, IL1β pre-treated biopsies decreased capacity for repair by hBMSCs and decreased levels of chondrogenic phenotype of human cartilage lesions. | es_ES |
dc.description.sponsorship | This study was supported by grants: CAM (S2009/MAT-1472); CIBER-BBN, Instituto de Salud Carlos III (CB06/01/0040); Rede Galega de Terapia Celular and Grupos con Potencial de Crecemento, Xunta de Galicia (R2016/036, R2014/050 and GPC2014/048); MINECO-FEDER (RTC-2016-5386-1); Universidade da Coruña; Fundación Española de Reumatología (2014 grant); Fundación Profesor Novoa Santos and Diputación da Coruña. Clara Sanjurjo-Rodríguez and Rocío Castro-Viñuelas are beneficiaries of fellowships (postdoctoral and predoctoral, respectively) from Xunta de Galicia. | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/CAM/Programa Nacional de Investigación Aplicada/S2009/MAT-1472/ES/Biocerámicas para ingeniería tisular | es_ES |
dc.description.sponsorship | Instituto de Salud Carlos III; CB06/01/0040 | es_ES |
dc.description.sponsorship | Xunta de Galicia; R2016/036 | es_ES |
dc.description.sponsorship | Xunta de Galicia; R2014/050 | es_ES |
dc.description.sponsorship | Xunta de Galicia; GPC2014/048 | es_ES |
dc.description.sponsorship | España. Ministerio de Economía y Competitividad; RTC-2016-5386-1 | |
dc.language.iso | eng | es_ES |
dc.publisher | Ivyspring | es_ES |
dc.relation.uri | http://dx.doi.org/10.7150/ijms.19835 | es_ES |
dc.rights | Creative Commond Attribution-NonCommercial 4.0 International Licence (CC-BY-NC 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject | Regenerative medicine | es_ES |
dc.subject | Tissue engineering | es_ES |
dc.subject | Hyaline cartilage | es_ES |
dc.subject | Tissue scaffolds | es_ES |
dc.subject | Mesenchymal stromal cells | es_ES |
dc.subject | Osteoarthritis | es_ES |
dc.title | Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | International Journal of Medical Sciences | es_ES |
UDC.volume | 14 | es_ES |
UDC.issue | 12 | es_ES |
UDC.startPage | 1257 | es_ES |
UDC.endPage | 1262 | es_ES |
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