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dc.contributor.authorMuiños-López, Emma
dc.contributor.authorHermida Gómez, Tamara
dc.contributor.authorFuentes Boquete, Isaac Manuel
dc.contributor.authorDe-Toro, Javier
dc.contributor.authorBlanco García, Francisco J
dc.contributor.authorDíaz-Prado, Silvia
dc.date.accessioned2017-09-22T12:06:42Z
dc.date.issued2017-01-10
dc.identifier.citationMuiños-López E, Hermida-Gómez T, Fuentes-Boquete I, de Toro-Santos J, Blanco FJ, Díaz-Prado SM. Human amniotic mesenchymal stromal cells as favorable source for cartilage repair. Tissue Eng Part A. 2017;23(17-18):901-912es_ES
dc.identifier.issn1937-3341
dc.identifier.issn1937-335X
dc.identifier.urihttp://hdl.handle.net/2183/19523
dc.description.abstract[Abstract] Introduction: Localized trauma-derived breakdown of the hyaline articular cartilage may progress toward osteoarthritis, a degenerative condition characterized by total loss of articular cartilage and joint function. Tissue engineering technologies encompass several promising approaches with high therapeutic potential for the treatment of these focal defects. However, most of the research in tissue engineering is focused on potential materials and structural cues, while little attention is directed to the most appropriate source of cells endowing these materials. In this study, using human amniotic membrane (HAM) as scaffold, we defined a novel static in vitro model for cartilage repair. In combination with HAM, four different cell types, human chondrocytes, human bone marrow-derived mesenchymal stromal cells (hBMSCs), human amniotic epithelial cells, and human amniotic mesenchymal stromal cells (hAMSCs) were assessed determining their therapeutic potential. Material and Methods: A chondral lesion was drilled in human cartilage biopsies simulating a focal defect. A pellet of different cell types was implanted inside the lesion and covered with HAM. The biopsies were maintained for 8 weeks in culture. Chondrogenic differentiation in the defect was analyzed by histology and immunohistochemistry. Results: HAM scaffold showed good integration and adhesion to the native cartilage in all groups. Although all cell types showed the capacity of filling the focal defect, hBMSCs and hAMSCs demonstrated higher levels of new matrix synthesis. However, only the hAMSCs-containing group presented a significant cytoplasmic content of type II collagen when compared with chondrocytes. More collagen type I was identified in the new synthesized tissue of hBMSCs. In accordance, hBMSCs and hAMSCs showed better International Cartilage Research Society scoring although without statistical significance. Conclusion: HAM is a useful material for articular cartilage repair in vitro when used as scaffold. In combination with hAMSCs, HAM showed better potential for cartilage repair with similar reparation capacity than chondrocytes.es_ES
dc.description.sponsorshipThis study was supported by grants: Servizo Galego de Saúde, Xunta de Galicia (PS07/84), Cátedra Bioibérica de la Universidade da Coruña, Instituto de Salud Carlos III, CIBERBBN, REDICENT (Rede de Investigación en Células Nai e Terapia Celular) and GPC (Grupos con Potencial de Crecemento) both from Xunta de Galicia (CN2012/142, R2014/050, and GPC2014/048); Rheumatology Spanish Foundation (FER 2014 grant). The results of this work have been funded by the Project No. PI16/02124, integrated in the National Plan for Scientific Research, Development and Technological Innovation 2013–2016 and funded by the ISCIII—General Subdirection of Assesment and Promotion of the Research— European Regional Development Fund (FEDER) ‘‘A way of making Europe"es_ES
dc.description.sponsorshipXunta de Galicia; PS07/84es_ES
dc.description.sponsorshipXunta de Galicia; CN2012/142es_ES
dc.description.sponsorshipXunta de Galicia; R2014/050es_ES
dc.description.sponsorshipXunta de Galicia; GPC2014/048
dc.description.sponsorshipinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/PI16%2F02124/ES/Determinación de índices predictivos de diagnóstico y pronóstico de artrosis de rodilla mediante la validación de biomarcadores proteicos
dc.language.isoenges_ES
dc.publisherMary Ann Liebertes_ES
dc.relation.urihttp://dx.doi.org/10.1089/ten.tea.2016.0422es_ES
dc.rightsFinal publication is avaliable from Mary Ann Liebert, Inc. publishers.es_ES
dc.subjectAmniotic membranees_ES
dc.subjectChondrocyteses_ES
dc.subjectBone marrow mesenchymal stromal cellses_ES
dc.subjectCartilagees_ES
dc.subjectAmniotic mesenchymal stromal cellses_ES
dc.subjectOsteoarthritises_ES
dc.subjectCell therapyes_ES
dc.subjectTissue engineeringes_ES
dc.titleHuman amniotic mesenchymal stromal cells as favorable source for cartilage repaires_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/embargoedAccesses_ES
dc.date.embargoEndDate2018-01-10es_ES
dc.date.embargoLift2018-01-10
UDC.journalTitleTissue Engineering Part Aes_ES
UDC.volume23es_ES
UDC.issue17-18es_ES
UDC.startPage901es_ES
UDC.endPage912es_ES


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