Mostrar o rexistro simple do ítem
The mitochondrial inhibitor oligomycin induces an inflammatory response in the rat knee joint
dc.contributor.author | Vaamonde-García, Carlos | |
dc.contributor.author | Loureiro, Jesús | |
dc.contributor.author | Valcárcel Ares, Marta Noa | |
dc.contributor.author | Riveiro Naveira, Romina R. | |
dc.contributor.author | Ramil-Gómez, Olalla | |
dc.contributor.author | Hermida-Carballo, Laura | |
dc.contributor.author | Centeno, Alberto | |
dc.contributor.author | Meijide-Faílde, Rosa | |
dc.contributor.author | Blanco García, Francisco J | |
dc.contributor.author | López-Armada, María José | |
dc.date.accessioned | 2017-08-25T11:23:22Z | |
dc.date.available | 2017-08-25T11:23:22Z | |
dc.date.issued | 2017-06-12 | |
dc.identifier.citation | Vaamonde-García C, Loureiro J, Valcárcel-Ares MN, Riveiro-Naveira RR, Ramil-Gómez O, Hermida-Carballo L, Centeno A, Meijide-Failde R, Blanco FJ, López-Armada MJ. The mitochondrial inhibitor oligomycin induces an inflammatory response in the rat knee joint. BMC Musculoskelet Disord. 2017 Jun 12;18(1):254. | es_ES |
dc.identifier.issn | 1471-2474 | |
dc.identifier.uri | http://hdl.handle.net/2183/19359 | |
dc.description.abstract | [Abstract] Background. Recent findings support a connection between mitochondrial dysfunction and activation of inflammatory pathways in articular cells. This study investigates in vivo in an acute model whether intra-articular administration of oligomycin, an inhibitor of mitochondrial function, induces an oxidative and inflammatory response in rat knee joints. Methods. Oligomycin was injected into the rat left knee joint on days 0, 2, and 5 before joint tissues were obtained on day 6. The right knee joint served as control. Results were evaluated by macroscopy and histopathology and by measuring cellular and mitochondrial reactive oxygen species (ROS), 4-hydroxy-2-nonenal (4-HNE, a marker of lipid peroxidation), nuclear factor erythroid 2-related factor 2 (Nrf2), and CD68 (macrophages) and chemokine levels. The marker of mitochondrial mass COX-IV was also evaluated. Results. The macroscopic findings showed significantly greater swelling in oligomycin-injected knees than in control knees. Likewise, the histological score of synovial damage was also increased significantly. Immunohistochemical studies showed high expression of IL-8, coinciding with a marked infiltration of polymorphonuclears and CD68+ cells in the synovium. Mitochondrial mass was increased in the synovium of oligomycin-injected joints, as well as cellular and mitochondrial ROS production, and 4-HNE. Relatedly, expression of the oxidative stress-related transcription factor Nrf2 was also increased. As expected, no histological differences were observed in the cartilage; however, cytokine-induced neutrophil chemoattractant-1 mRNA and protein expression were up-regulated in this tissue. Conclusions. Mitochondrial failure in the joint is able to reproduce the oxidative and inflammatory status observed in arthritic joints. | es_ES |
dc.description.sponsorship | This work was supported by grants from Fondo Investigación Sanitaria-Spain [09/02340, PI12/02771 and RD12/0009/0018, RIER] and Secretaría Xeral I + D + I [PXIB916357PR, PS09/56, INCITE 09E1R916139ES, IN845B2010/176,10CSA916035PR]. MJ.L-A was supported by Contrato Investigadores Sistema Nacional de Salud [Fondo Investigación Sanitaria, Spain CP06/00292], J.L by Contrato Sara Borrel [Fondo Investigación Sanitaria, Spain CD12/00648], L.H-C by Ministerio de Economía y Competitividad [MINECO, PTA 2013-8278-I]. C.V-G, RR. R-N, and MN.V-A are recipients of a grant from Secretaría Xeral I + D + I (Plan I2, PS09/56, Programa María Barbeito 2008, respectively). O.R-G was supported by a grant from Diputación de A Coruña. | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MICINN/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/PS09%2F02340/ES/IMPLICACION DE LA DISFUNCION MITOCONDRIAL EN LA RESPUESTA INFLAMATORIA INDUCIDA POR CITOQUINAS EN SINOVIOCITOS: PAPEL DE LAS SIRTUINAS. INTERVENCION PREVENTIVA O TERAPEUTICA CON UNA DIETA ANTIOXIDANTE | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Acción Estratégica de Salud/PI12%2F02771/ES/Implicación del factor de transcripción Nrf2 en la angiogénesis sinovial de la artritis reumatoide. Efecto de una dieta antioxidante | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Acción Estratégica de Salud/RD12%2F0009%2F0018/ES/Inflamación y enfermedades reumáticas | |
dc.description.sponsorship | Xunta de Galicia; PXIB916357PR | |
dc.description.sponsorship | Xunta de Galicia; PS09/56 | |
dc.description.sponsorship | Xunta de Galicia; INCITE 09E1R916139ES | |
dc.description.sponsorship | Xunta de Galicia; IN845B2010/176 | |
dc.description.sponsorship | Xunta de Galicia; 10CSA916035PR | |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.relation.uri | http://dx.doi.org/10.1186/s12891-017-1621-2 | es_ES |
dc.rights | Creative Commons Attribution 4.0 International License (CC-BY 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Mitochondria | es_ES |
dc.subject | Inflammation | es_ES |
dc.subject | Oxidative stress | es_ES |
dc.subject | Synovial tissue | es_ES |
dc.subject | Cartilage | es_ES |
dc.title | The mitochondrial inhibitor oligomycin induces an inflammatory response in the rat knee joint | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | BMC Musculoskletal Disorders | es_ES |
UDC.volume | 18 | es_ES |
UDC.issue | 1 | es_ES |
UDC.startPage | 254 | es_ES |
Ficheiros no ítem
Este ítem aparece na(s) seguinte(s) colección(s)
-
GI-TCMR - Artigos [130]
-
INIBIC-TCMR - Artigos [102]
-
INIBIC- REUMA - Artigos [184]