Show simple item record

dc.contributor.authorFuente, Alexandre de la
dc.contributor.authorMateos, Jesús
dc.contributor.authorLesende-Rodríguez, Iván
dc.contributor.authorCalamia, Valentina
dc.contributor.authorFuentes Boquete, Isaac Manuel
dc.contributor.authorDe-Toro, Javier
dc.contributor.authorArufe, M.C.
dc.contributor.authorBlanco García, Francisco J
dc.date.accessioned2017-03-28T11:37:23Z
dc.date.available2017-03-28T11:37:23Z
dc.date.issued2011-10-17
dc.identifier.citationFuente A, Mateos J, Lesende-Rodríguez I, et al. Proteome analysis during chondrocyte differentiation in a new chondrogenesis model using human umbilical cord stroma mesenchymal stem cells. Mol Cell Proteomics. 2012;11(2)es_ES
dc.identifier.issn1535-9476
dc.identifier.issn1535-9484
dc.identifier.urihttp://hdl.handle.net/2183/18340
dc.description.abstract[Abstract] Umbilical cord stroma mesenchymal stem cells were differentiated toward chondrocyte-like cells using a new in vitro model that consists of the random formation of spheroids in a medium supplemented with fetal bovine serum on a nonadherent surface. The medium was changed after 2 days to one specific for the induction of chondrocyte differentiation. We assessed this model using reverse transcriptase-polymerase chain reaction, flow cytometry, immunohistochemistry, and secretome analyses. The purpose of this study was to determine which proteins were differentially expressed during chondrogenesis. Differential gel electrophoresis analysis was performed, followed by matrix-assisted laser desorption/ionization mass spectrometry protein identification. A total of 97 spots were modulated during the chondrogenesis process, 54 of these spots were identified as 39 different proteins and 15 were isoforms. Of the 39 different proteins identified 15 were down-regulated, 21 were up-regulated, and 3 were up- and down-regulated during the chondrogenesis process. Using Pathway Studio 7.0 software, our results showed that the major cell functions modulated during chondrogenesis were cellular differentiation, proliferation, and migration. Five proteins involved in cartilage extracellular matrix metabolism found during the differential gel electrophoresis study were confirmed using Western blot. The results indicate that our in vitro chondrogenesis model is an efficient and rapid technique for obtaining cells similar to chondrocytes that express proteins characteristic of the cartilage extracellular matrix. These chondrocyte-like cells could prove useful for future cell therapy treatment of cartilage pathologies.es_ES
dc.description.sponsorshipMinisterio de Ciencia en Innovacion; PLE2009–0144es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Biochemistry and Molecular Biologyes_ES
dc.relation.urihttp://dx.doi.org/10.1074/mcp.M111.010496es_ES
dc.titleProteome Analysis During Chondrocyte Differentiation in a New Chondrogenesis Model Using Human Umbilical Cord Stroma Mesenchymal Stem Cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleMolecular & Cellular Proteomicses_ES
UDC.volume11es_ES
UDC.issue2es_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record