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Ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineering
dc.contributor.author | Sanjurjo-Rodríguez, Clara | |
dc.contributor.author | Castro Viñuelas, Rocío | |
dc.contributor.author | Hermida Gómez, Tamara | |
dc.contributor.author | Fernández Vázquez, Tania | |
dc.contributor.author | Fuentes Boquete, Isaac Manuel | |
dc.contributor.author | De-Toro, Javier | |
dc.contributor.author | Díaz-Prado, Silvia | |
dc.contributor.author | Blanco García, Francisco J | |
dc.date.accessioned | 2017-02-15T13:00:59Z | |
dc.date.available | 2017-02-15T13:00:59Z | |
dc.date.issued | 2017-01-31 | |
dc.identifier.citation | Sanjurjo-Rodríguez C, Castro-Viñuelas R, Hermida-Gómez T, Fernández-Vázquez T, Fuentes-Boquete IM, de Toro-Santos FJ, Díaz-Prado SM, Blanco-García FJ. Ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineering. PLoS One. 2017 Jan 31;12(1):e0171231. | es_ES |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/2183/18118 | |
dc.description.abstract | [Abstract] Introduction. Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral diseases. Purpose. The aim was to isolate and characterize oMSCs from bone marrow aspirates (oBMSCs) and to assess their usefulness for osteochondral repair using β-tricalcium phosphate (bTCP) and type I collagen (Col I) scaffolds. Methods. Cells isolated from ovine bone marrow were characterized morphologically, phenotypically, and functionally. oBMSCs were cultured with osteogenic medium on bTCP and Col I scaffolds. The resulting constructs were evaluated by histology, immunohistochemistry and electron microscopy studies. Furthermore, oBMSCs were cultured on Col I scaffolds to develop an in vitro cartilage repair model that was assessed using a modified International Cartilage Research Society (ICRS) II scale. Results. oBMSCs presented morphology, surface marker pattern and multipotent capacities similar to those of human BMSCs. oBMSCs seeded on Col I gave rise to osteogenic neotissue. Assessment by the modified ICRS II scale revealed that fibrocartilage/hyaline cartilage was obtained in the in vitro repair model. Conclusions. The isolated ovine cells were demonstrated to be oBMSCs. oBMSCs cultured on Col I sponges successfully synthesized osteochondral tissue. The data suggest that oBMSCs have potential for use in preclinical models prior to human clinical studies. | es_ES |
dc.description.sponsorship | This study was supported by grants: ICIRO, Servizo Galego de Saúde; Universidade da Coruña (UDC); Cátedra Bioiberica from UDC; CAM (S2009/MAT-1472); CIBER-BBN from Instituto de Salud Carlos III; Rede de Investigación en Células Nai e Terapia Celular (REDICENT), Xunta de Galicia (R2014/050); GPC, Xunta de Galicia (GPC2014/048); Fundación Española de Reumatología (2014 grant); R. Castro-Viñuelas was beneficiary of a grant from Diputación de A Coruña (Spain). | |
dc.description.sponsorship | info:eu-repo/grantAgreement/CAM/Programa Nacional de Investigación Aplicada/S2009%2FMAT-1472/ES/Biocerámicas para ingeniería tisular | |
dc.description.sponsorship | Xunta de Galicia; R2014/050 | |
dc.description.sponsorship | Xunta de Galicia; GPC2014/048 | |
dc.language.iso | eng | es_ES |
dc.publisher | PLoS ONE | es_ES |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0171231 | es_ES |
dc.rights | Creative Commons Attribution 4.0 International Licence (CC-BY 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineering | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | PLoS One | es_ES |
UDC.volume | 12 | es_ES |
UDC.issue | 1 | es_ES |
UDC.startPage | e0171231 | es_ES |
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