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Molecular profile and cellular characterization of human bone marrow mesenchymal stem cells: donor influence on chondrogenesis
dc.contributor.author | Cicione, Claudia | |
dc.contributor.author | Díaz-Prado, Silvia | |
dc.contributor.author | Muiños-López, Emma | |
dc.contributor.author | Hermida Gómez, Tamara | |
dc.contributor.author | Blanco García, Francisco J | |
dc.date.accessioned | 2015-06-22T08:55:18Z | |
dc.date.available | 2015-06-22T08:55:18Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Cicione C, Díaz-Prado C, Muíños-López E, Hermida-Gómez T, Blanco FJ. Molecular profile and cellular characterization of human bone marrow mesenchymal stem cells: donor influence on chondrogenesis. Differentiation. 2010;80(2-3):155-165. | es_ES |
dc.identifier.uri | http://hdl.handle.net/2183/14711 | |
dc.description.abstract | [Abstract] Background. The use of autologous or allogenic stem cells has recently been suggested as an alternative therapeutic approach for treatment of cartilage defects. Bone marrow mesenchymal stem cells (BM-MSCs) are well-characterized multipotent cells that can differentiate into different cell types. Understanding the potential of these cells and the molecular mechanisms underlying their differentiation should lead to innovative protocols for clinical applications. The aim of this study was to evaluate the usefulness of surface antigen selection of BM-MSCs and to understand the mechanisms underlying their differentiation. Methods. MSCs were isolated from BM stroma and expanded. CD105+ subpopulation was isolated using a magnetic separator. We compared culture-expanded selected cells with non-selected cells. We analyzed the phenotypic profiles, the expression of the stem cell marker genes Nanog, Oct3/4, and Sox2 and the multi-lineage differentiation potential (adipogenic, osteogenic, and chondrogenic). The multi-lineage differentiation was confirmed using histochemistry, immunohistochemistry and/or real-time polymerase chain reaction (qPCR) techniques. Results. The selected and non-selected cells displayed similar phenotypes and multi-lineage differentiation potentials. Analyzing each cell source individually, we could divide the six donors into two groups: one with a high percentage of CD29 (β1-integrin) expression (HL); one with a low percentage of CD29 (LL). These two groups had different chondrogenic capacities and different expression levels of the stem cell marker genes. Conclusions. This study showed that phenotypic profiles of donors were related to the chondrogenic potential of human BM-MSCs. The chondrogenic potential of donors was related to CD29 expression levels. The high expression of CD29 antigen seemed necessary for chondrogenic differentiation. Further investigation into the mechanisms responsible for these differences in BM-MSCs chondrogenesis is therefore warranted. Understanding the mechanisms for these differences will contribute to improved clinical use of autologous human BM-MSCs for articular cartilage repair. | es_ES |
dc.description.sponsorship | This study was supported by grants from Servizo Galego de Saúde, Xunta de Galicia (PS07/84), Catedra Bioiberica de la Universidade da Coruña, and Instituto de Salud Carlos III CIBER BBN CB06-01-0040. Claudia Cicione was supported by the CHU A Coruña Foundation, Spain. Silvia Díaz-Prado is the beneficiary of an Isidro Parga Pondal contract from Xunta de Galicia, A Coruña, Spain. Tamara Hermida-Gómez is the holder of a contract from Fondo de Investigación Sanitaria (2008), Spain. Emma Muíños-López is supported by the Rheumatology Spanish Foundation, Spain. We would like to thank M.J. Sánchez and P. Filgueira for technical assistance. | es_ES |
dc.description.sponsorship | Instituto de Salud Carlos III; CB06-01-0040 | |
dc.description.sponsorship | Xunta de Galicia; PS07/84 | |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.uri | http://dx.doi.org/10.1016/j.diff.2010.06.001 | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs International Licence (CC-BY-NC-ND 4.0) | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Bone marrow | es_ES |
dc.subject | Chondrogenesis | es_ES |
dc.subject | Cartilage | es_ES |
dc.subject | Osteoarthritis | es_ES |
dc.subject | Cell therapy | es_ES |
dc.title | Molecular profile and cellular characterization of human bone marrow mesenchymal stem cells: donor influence on chondrogenesis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
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