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dc.contributor.authorCicione, Claudia
dc.contributor.authorMuiños Gómez, Emma
dc.contributor.authorHermida Gómez, Tamara
dc.contributor.authorFuentes Boquete, Isaac Manuel
dc.contributor.authorDíaz-Prado, Silvia
dc.contributor.authorBlanco García, Francisco J
dc.date.accessioned2015-06-11T09:48:18Z
dc.date.issued2014-09-10
dc.identifier.citationCiclione C, Muiños-López E, Hermida-Gómez T, Fuentes-Boquete I, Díaz-Prado S, Blanco FJ. Alternative protocols to induce chondrogenic differentiation: transforming growth factor-β superfamily. Cell Tissue Bank. 2015;16:195-207es_ES
dc.identifier.urihttp://hdl.handle.net/2183/14659
dc.description.abstract[Abstract] Mesenchymal stem cells (MSCs) are an accepted candidate for cell-based therapy of multiple diseases. The interest in MSCs and their possible application in cell therapy have resulted in a better understanding of the basic biology of these cells. Recently, like aggregation and transforming growth factor beta (TGFβ) delivery, hypoxia has been indicated as crucial for complete chondrogenesis. The aim of this study was to test different culture conditions for directing stem cell differentiation into the chondrogenic lineage in vitro by testing different TGFβ superfamily members into the culture media under normoxic conditions. All chondrogenic culture conditions used allowed the differentiation of bone marrow-MSCs (BM-MSCs) into chondrogenic lineage. Chondrogenic induction capacity depended on the growth factor added to the culture media. In particular, the chondrogenic culture condition that better induced chondrogenesis was the medium that included the combination of three growth factors: bone morphogenetic protein-2 (BMP-2), BMP-7 and TGFβ-3. In this culture media, differentiated cells showed the highest levels expression of two markers of chondrogenesis, SOX9 and COL2A1, compared to the control points (p < 0.05, two-tailed t test). In our experimental conditions, the combination of BMP-2, BMP-7 and TGFβ-3 was the most effective in promoting chondrogenesis of BM-MSCs. These results underline the importance of determining in each experimental design the best protocol for in vitro directing stem cell differentiation into the chondrogenic lineage.es_ES
dc.description.sponsorshipThis study was supported by grants: Servizo Galego de Saúde, Xunta de Galicia (PS07/84), Cátedra Bioiberica de la Universidade da Coruña and Instituto de Salud Carlos III CIBER BBN; Ministerio Ciencia e Innovacion PLE2009-0144; Fondo Investigacion Sanitaria-PI 08/2028 with participation of funds from FEDER (European Community), Tamara Hermida-Gómez is the beneficiary of a contract from Fondo de Investigación Sanitaria (2008), Spain. We would like to thank P.Filgueira and M.J.Sánchez for technical assistance.es_ES
dc.description.sponsorshipXunta de Galicia; PS07/84
dc.description.sponsorshipinfo:eu-repo/grantAgreement/MICINN/Programa Nacional de Internacionalización de la I+D/PLE2009-0144/ES/In situ Tissue Engineering using Stem Cells and Functional Biomaterials to Repair Articular Cartilage: An ''in Vivo Model"
dc.description.sponsorshipInstituto de Salud Carlos III; PI08/2028
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.urihttp://dx.doi.org/10.1007/s10561-014-9472-7es_ES
dc.rightsThe final publication is avaliable at link.springer.comes_ES
dc.subjectCell therapyes_ES
dc.subjectAutologous chondrocyte implantationes_ES
dc.subjectFocal lesionses_ES
dc.subjectHyalinees_ES
dc.titleAlternative protocols to induce chondrogenic differentiation: transforming growth factor-β superfamilyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
dc.date.embargoEndDate2015-09-10es_ES
dc.date.embargoLift2015-09-10


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