Mostrar o rexistro simple do ítem
Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications
dc.contributor.author | Brosel, Sergi | |
dc.contributor.author | Rius, Gemma | |
dc.contributor.author | Aviñó, Ana | |
dc.contributor.author | Nako, Nakatsuka | |
dc.contributor.author | Illa, Xavi | |
dc.contributor.author | Corro, Elena del | |
dc.contributor.author | Delgà-Fernández, Marta | |
dc.contributor.author | Criado, Alejandro | |
dc.contributor.author | Guimerà, Anton | |
dc.contributor.author | Prats Alfonso, Elisabet | |
dc.date.accessioned | 2024-06-21T17:30:43Z | |
dc.date.available | 2024-06-21T17:30:43Z | |
dc.date.issued | 2023-12-10 | |
dc.identifier.citation | S. Brosel-Oliu, G. Rius, A. Aviñó, N. Nakatsuka, X. Illa, E. del Corro, M. Delgà-Fernández, E. Masvidal-Codina, N. Rodríguez, J. P. Merino, A. Criado, M. Prato, R. Tkatchenko, R. Eritja, P. Godignon, J. A. Garrido, R. Villa, A. Guimerà, E. Prats-Alfonso, Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications. Small 2024, 20, 2308857. https://doi.org/10.1002/smll.202308857 | es_ES |
dc.identifier.issn | 1613-6810 | |
dc.identifier.issn | 1613-6829 (E-ISSN) | |
dc.identifier.uri | http://hdl.handle.net/2183/37315 | |
dc.description.abstract | [Abstract]: Graphene solution-gated field-effect transistors (gSGFETs) offer high potential for chemical and biochemical sensing applications. Among the current trends to improve this technology, the functionalization processes are gaining relevance for its crucial impact on biosensing performance. Previous efforts are focused on simplifying the attachment procedure from standard multi-step to single-step strategies, but they still suffer from overreaction, and impurity issues and are limited to a particular ligand. Herein, a novel strategy for single-step immobilization of chemically modified aptamers with fluorenylmethyl and acridine moieties, based on a straightforward synthetic route to overcome the aforementioned limitations is presented. This approach is benchmarked versus a standard multi-step strategy using thrombin as detection model. In order to assess the reliability of the functionalization strategies 48-gSGFETs arrays are employed to acquire large datasets with multiple replicas. Graphene surface characterization demonstrates robust and higher efficiency in the chemical coupling of the aptamers with the single-step strategy, while the electrical response evaluation validates the sensing capability, allowing to implement different alternatives for data analysis and reduce the sensing variability. In this work, a new tool capable of overcome the functionalization challenges of graphene surfaces is provided, paving the way toward the standardization of gSGFETs for biosensing purposes. | es_ES |
dc.description.sponsorship | This work has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement no. 881603 (GrapheneCore3) and no. 785219 (GrapheneCore2). M. P. is AXA Professor and is supported by the European Research Council (ERC-AdG-2019, no. 885323), the Agencia Estatal de Investigación-AEI (“Proyectos I+D+i 2019-Modalidad Retos Investigación”, no. PID2019-108523RB-I00), by grant PRE2020-095099 funded by MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your future.” Part of this work has made use of the Spanish ICTS Network MICRONANOFABS, partially supported by MICINN and the ICTS NANBIOSIS, more specifically by the Micro-NanoTechnology Unit U8 of the CIBER-BBN. This project was also funded by the Generalitat de Catalunya (2021SGR00495), by the Spanish Ministerio de Ciencia e Innovación (PID2021-126117NA-I00), by “ERDF A way of making Europe”, and by CIBER-BBN (CB06/01/0049). X. I., R. V., A. G. and E. P.-A. thank the financial support provided by CIBER-BBN and the Instituto de Salud Carlos III with assistance from the European Regional Development. A. C. acknowledges financial support by Grant ED431H 2020/17 funded by Xunta de Galicia, and by Grant RYC2020-030183-I funded by MCIN/AEI/10.13039/501100011033 and “ESF Investing in your future”. | es_ES |
dc.description.sponsorship | Generalitat de Catalunya; 2021SGR00495 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley-VCH | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/785219 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/881603 | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC2019-027879-I/ES/ | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC2020-030183-I/ES/ | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MDM-2017-0720/ES/ | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108523RB-I00/ES/NANODOTS DE CARBONO A MEDIDA COMO NUEVOS MATERIALES MULTIFUNCIONALES SEGUROS PARA APLICACIONES NANO- Y BIO-TECNOLOGICAS | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126117NA-I00/ES/INTERFASES MULTIMODALES BASADAS EN GRAFENO PARA REGISTRAR LA ACTIVIDAD CEREBRAL | es_ES |
dc.relation | info:eu-repo/grantAgreement/MSC/Programa Nacional de Recursos y Tecnologías Agroalimentarias/CB06%2F01%2F0019/ES/Desarrollo e implementación de nuevas tecnologías en biomedicina 19 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MSC/Programa Nacional de Recursos y Tecnologías Agroalimentarias/CB06%2F01%2F0049/ES/Desarrollo e implementación de nuevas tecnologías en biomedicina 49 | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC2018-024454-I/ES/ | es_ES |
dc.relation.uri | https://doi.org/10.1002/smll.202308857 | es_ES |
dc.rights | © 2023 The Authors. Small published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (made. https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | es_ES |
dc.subject | Array | es_ES |
dc.subject | Biosensor | es_ES |
dc.subject | Chemical functionalization | es_ES |
dc.subject | Field-effect transistor | es_ES |
dc.subject | Graphene | es_ES |
dc.subject | Microfabrication | es_ES |
dc.subject | Solution-gated | es_ES |
dc.title | Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Small | es_ES |
UDC.volume | 20 | es_ES |
UDC.issue | 18 | es_ES |
UDC.startPage | Article 2308857 | es_ES |
dc.identifier.doi | 10.1002/smll.202308857 |
Ficheiros no ítem
Este ítem aparece na(s) seguinte(s) colección(s)
-
OpenAIRE [332]
-
GI- NANOSELF4BIO - Artigos [30]