Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications
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Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing ApplicationsAutor(es)
Data
2023-12-10Cita bibliográfica
S. Brosel-Oliu, G. Rius, A. Aviñó, N. Nakatsuka, X. Illa, E. del Corro, M. Delgà-Fernández, E. Masvidal-Codina, N. Rodríguez, J. P. Merino, A. Criado, M. Prato, R. Tkatchenko, R. Eritja, P. Godignon, J. A. Garrido, R. Villa, A. Guimerà, E. Prats-Alfonso, Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications. Small 2024, 20, 2308857. https://doi.org/10.1002/smll.202308857
Resumo
[Abstract]: Graphene solution-gated field-effect transistors (gSGFETs) offer high potential for chemical and biochemical sensing applications. Among the current trends to improve this technology, the functionalization processes are gaining relevance for its crucial impact on biosensing performance. Previous efforts are focused on simplifying the attachment procedure from standard multi-step to single-step strategies, but they still suffer from overreaction, and impurity issues and are limited to a particular ligand. Herein, a novel strategy for single-step immobilization of chemically modified aptamers with fluorenylmethyl and acridine moieties, based on a straightforward synthetic route to overcome the aforementioned limitations is presented. This approach is benchmarked versus a standard multi-step strategy using thrombin as detection model. In order to assess the reliability of the functionalization strategies 48-gSGFETs arrays are employed to acquire large datasets with multiple replicas. Graphene surface characterization demonstrates robust and higher efficiency in the chemical coupling of the aptamers with the single-step strategy, while the electrical response evaluation validates the sensing capability, allowing to implement different alternatives for data analysis and reduce the sensing variability. In this work, a new tool capable of overcome the functionalization challenges of graphene surfaces is provided, paving the way toward the standardization of gSGFETs for biosensing purposes.
Palabras chave
Array
Biosensor
Chemical functionalization
Field-effect transistor
Graphene
Microfabrication
Solution-gated
Biosensor
Chemical functionalization
Field-effect transistor
Graphene
Microfabrication
Solution-gated
Versión do editor
Dereitos
© 2023 The Authors. Small published by Wiley-VCH GmbH. This is an
open access article under the terms of the Creative Commons
Attribution-NonCommercial-NoDerivs License (made.
https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and
distribution in any medium, provided the original work is properly cited,
the use is non-commercial and no modifications or adaptations are made.
ISSN
1613-6810
1613-6829 (E-ISSN)
1613-6829 (E-ISSN)