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Methylphenidate promotes premature growth plate closure: in vitro evidence
dc.contributor.author | Pazos-Pérez, Andrés | |
dc.contributor.author | Piñeiro-Ramil, María | |
dc.contributor.author | Franco-Trepat, Eloi | |
dc.contributor.author | Guillán-Fresco, María | |
dc.contributor.author | López-López, Verónica | |
dc.contributor.author | Jorge-Mora, Alberto | |
dc.contributor.author | Alonso-Pérez, Ana | |
dc.contributor.author | Gómez, Rodolfo | |
dc.date.accessioned | 2024-01-24T11:15:40Z | |
dc.date.available | 2024-01-24T11:15:40Z | |
dc.date.issued | 2023-02-20 | |
dc.identifier.citation | Pazos-Pérez A, Piñeiro-Ramil M, Franco-Trepat E, Guillán-Fresco M, López-López V, Jorge-Mora A, Alonso-Pérez A, Gómez R. Methylphenidate promotes premature growth plate closure: in vitro evidence. Int J Mol Sci. 2023 Feb 20;24(4):4175. | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/2183/35106 | |
dc.description.abstract | [Abstract] It is well known that patients with attention deficit hyperactivity disorder treated with stimulants, such as methylphenidate hydrochloride (MPH), have reduced height and weight. Even though MPH has an anorexigenic effect, an additional impact of this drug on the growth plate cannot be discarded. In this study, we aimed to determine the cellular effect of MPH on an in vitro growth plate model. We tested the effects of MPH on the viability and proliferation of a prechondrogenic cell line via an MTT assay. In vitro differentiation of this cell line was performed, and cell differentiation was evaluated through the expression of cartilage- and bone-related genes as measured via RT-PCR. MPH did not alter the viability or proliferation of prechondrogenic cells. However, it reduced the expression of cartilage extracellular matrix-related genes (type II collagen and aggrecan) and increased the expression of genes involved in growth plate calcification (Runx2, type I collagen, and osteocalcin) at different phases of their differentiation process. Our results evidence that MPH upregulates genes associated with growth plate hypertrophic differentiation. This may induce premature closure of the growth plate, which would contribute to the growth retardation that has been described to be induced by this drug. | es_ES |
dc.description.sponsorship | This work was funded by the Instituto de Salud Carlos III (ISCIII) through the projects with grant numbers PT20/00009, FI20/00210, CM20/00186, RD21/0002/0025, PI19/01446, PI16/01870, CPII20/00026, CP15/00007, co-funded by the European Union via “Fondo de Investigación Sanitaria” from Fondo Europeo de Desarrollo Regional (FEDER); the Spanish Ministry of Science via FECYT (grant number PRECIPITA PR250); the Mutua Madrileña Foundation (grant numbers MMA 2018 and MMA 2020); and the Ministry of Universities (grant numbers FPU17/01706 and UDC Margarita Salas RSU.UDC.MS06, funded by the European Union through NextGenerationEU). Support was also provided to: E.F.-T. (MMA 2018), M.G.-F. (FPU17/01706), A.A.-P. (IDIS 2019), M.L.-F. (FI20/00210), A.P.-P. (MMA 2020), A.C.-G. (Rio Hortega CM20/00186), A.J.-M. (SERGAS, no grant number), S.B.B. (IDIS, no grant number), O.G. (SERGAS no grant number), R.G. (ISCIII and SERGAS via Miguel Servet II program CPII20/00026), V.L-L. (PT20/00009), and M.P.-R. (UDC Margarita Salas RSU.UDC.MS06). | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/ISCIII/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/PI19%2F01446/ES/CARACTERIZACION CELULAR CIRCULANTE E INHIBICION FARMACOLOGICA DEL CRECIMIENTO ANOMALO DEL HUESO ASOCIADO A LA OSIFICACION HETEROTOPICA | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/PI16%2F01870/ES/Estudio de los factores de transcripción asociados a los cambios en la metilación del ADN durante la osteoblastogénesis y su papel en la diferenciación del osteoblasto | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/CP15%2F00007/ES | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation.uri | https://doi.org/10.3390/ijms24044175 | es_ES |
dc.rights | Creative Commons Attribution 4.0 International License (CC-BY 4.0) | es_ES |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | ADHD | es_ES |
dc.subject | Bone | es_ES |
dc.subject | Cartilage | es_ES |
dc.subject | Chondrocytes | es_ES |
dc.subject | Endochondral ossification | es_ES |
dc.subject | Stimulants | es_ES |
dc.title | Methylphenidate promotes premature growth plate closure: in vitro evidence | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | International Journal of Molecular Sciences | es_ES |
UDC.volume | 24 | es_ES |
UDC.issue | 4 | es_ES |
UDC.startPage | 4175 | es_ES |
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