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In vitro study of the therapeutic potential of brown crude fucoidans in osteoarthritis treatment
dc.contributor.author | Vaamonde-García, Carlos | |
dc.contributor.author | Capelo Mera, Emma | |
dc.contributor.author | Flórez-Fernández, Noelia | |
dc.contributor.author | Torres, María Dolores | |
dc.contributor.author | Rivas-Murias, Beatriz | |
dc.contributor.author | Meijide-Faílde, Rosa | |
dc.contributor.author | Blanco García, Francisco J | |
dc.contributor.author | Domínguez, Herminia | |
dc.date.accessioned | 2022-12-07T08:34:21Z | |
dc.date.available | 2022-12-07T08:34:21Z | |
dc.date.issued | 2022-11-17 | |
dc.identifier.citation | Vaamonde-García C, Capelo-Mera E, Flórez-Fernández N, Torres MD, Rivas-Murias B, Mejide-Faílde R, Blanco FJ, Domínguez H. In vitro study of the therapeutic potential of brown crude fucoidans in osteoarthritis treatment. Int J Mol Sci. 2022 Nov 17;23(22):14236. | es_ES |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | http://hdl.handle.net/2183/32161 | |
dc.description.abstract | [Abstract] Osteoarthritis, one of the most common joint degenerative pathologies, still has no cure, and current treatments, such as nonsteroidal anti-inflammatory drugs, can cause serious adverse effects when taken for a long time. Brown seaweed crude fucoidans are used for the clinical treatment of several pathologies. In this study, the therapeutical potential of these biocompounds was analyzed in primary chondrocytes and the 260TT human chondrocyte cell line. Crude fucoidan from Undaria pinnatifida (Up) and Sargassum muticum (Sm) was obtained by different extraction techniques (microwave-assisted extraction, pressurized hot-water extraction, ultrasound-assisted extraction) and chemically and structurally characterized by Fourier transform infrared spectroscopy, high-performance size-exclusion chromatography, proton nuclear magnetic resonance, and scanning electron microscopy. Once cell viability was confirmed in chondrocytes treated with crude fucoidans, we evaluated their anti-inflammatory effects, observing a significant reduction in IL-6 production stimulated by IL-1β. Findings were confirmed by analysis of IL-6 and IL-8 gene expression, although only fucoidans from Up achieved a statistically significant reduction. Besides this, the antioxidant capacity of crude fucoidans was observed through the upregulation of Nrf-2 levels and the expression of its transcriptional target genes HO-1 and SOD-2, with compounds from Up again showing a more consistent effect. However, no evidence was found that crude fucoidans modulate senescence, as they failed to reduced β-galactosidase activity, cell proliferation, or IL-6 production in chondrocytes stimulated with etoposide. Thus, the findings of this research seem to indicate that the tested crude fucoidans are capable of partially alleviating OA-associated inflammation and oxidative stress, but fail to attenuate chondrocyte senescence. | es_ES |
dc.description.sponsorship | The study was supported by grant IN607A 2021/7 (Grupos de Referencia Competitiva) from Axencia Galega de Innovación-Xunta de Galicia, grant RICORS-REI, RD21/0002/0009 co-funded by ERDF/ESF, “A way to make Europe”/“Investing in your future”) and by the Biomedical Research Network Centre (CIBER). M.D.T. acknowledges to the Ministry of Science, Innovation and Universities of Spain for its postdoctoral grants (RYC2018-024454-I) and to the Consellería de Cultura, Educación e Universidade da Xunta de Galicia (ED431F 2020/01). N.F.-F. thanks Xunta de Galicia for her postdoctoral contract (grant number ED481D-2022-018). CIBER is an initiative of ISCIII. | |
dc.description.sponsorship | Xunta de Galicia; IN607A2021/7 | |
dc.description.sponsorship | Xunta de Galicia; RD21/0002/0009 | |
dc.description.sponsorship | España. Ministerio de Ciencia, Innovación y Universidades; RYC2018-024454-I | |
dc.description.sponsorship | Xunta de Galicia; ED431F2020/01 | |
dc.description.sponsorship | Xunta de Galicia; ED481D-2022-018 | |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation.uri | https://doi.org/10.3390/ijms232214236 | es_ES |
dc.rights | Creative Commons Attribution 4.0 International License (CC-BY 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Chondrocytes | es_ES |
dc.subject | Crude fucoidans | es_ES |
dc.subject | Hemeoxygenase 1 | es_ES |
dc.subject | Nuclear factor-erythroid 2-related factor | es_ES |
dc.subject | Osteoarthritis | es_ES |
dc.subject | Proinflammatory cytokines | es_ES |
dc.subject | Reactive oxygen species | es_ES |
dc.subject | Senescence | es_ES |
dc.title | In vitro study of the therapeutic potential of brown crude fucoidans in osteoarthritis treatment | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | International Journal of Molecular Sciences | es_ES |
UDC.volume | 23 | es_ES |
UDC.issue | 22 | es_ES |
UDC.startPage | 14236 | es_ES |
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