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dc.contributor.authorRodríguez-Fernández, Silvia
dc.contributor.authorÁlvarez-Portela, Marcelino
dc.contributor.authorRendal Vázquez, María Esther
dc.contributor.authorPiñeiro-Ramil, María
dc.contributor.authorSanjurjo-Rodríguez, Clara
dc.contributor.authorCastro Viñuelas, Rocío
dc.contributor.authorSánchez-Ibáñez, Jacinto
dc.contributor.authorFuentes Boquete, Isaac Manuel
dc.contributor.authorDíaz-Prado, Silvia
dc.date.accessioned2021-11-29T08:27:12Z
dc.date.available2021-11-29T08:27:12Z
dc.date.issued2021-11-22
dc.identifier.citationRodríguez-Fernández S, Álvarez-Portela M, Rendal-Vázquez E, Piñeiro-Ramil M, Sanjurjo-Rodríguez C, Castro-Viñuelas R, Sánchez-Ibáñez J, Fuentes-Boquete I, Díaz-Prado S. Analysis of cryopreservation protocols and their harmful effects on the endothelial integrity of human corneas. Int J Mol Sci. 2021 Nov 22;22(22):12564.es_ES
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/2183/28978
dc.description.abstract[Abstract] Corneal cryopreservation can partially solve the worldwide concern regarding donor cornea shortage for keratoplasties. In this study, human corneas were cryopreserved using two standard cryopreservation protocols that are employed in the Tissue Bank of the Teresa Herrera Hospital (Spain) to store corneas for tectonic keratoplasties (TK protocol) and aortic valves (AV protocol), and two vitrification protocols, VS55 and DP6. Endothelial viability and general corneal state were evaluated to determine the protocol that provides the best results. The potential corneal cryopreservation protocol was studied in detail taking into consideration some cryopreservation-related variables and the endothelial integrity and stroma arrangement of the resulting cryopreserved corneas. TK corneas showed mostly viable endothelial cells, while the others showed few (AV) or none (DP6 and VS55). The corneal structure was well maintained in TK and AV corneas. TK corneas showed endothelial acellular areas surrounded by injured cells and a normal-like stromal fiber arrangement. Cryoprotectant solutions of the TK protocol presented an increasing osmolality and a physiological pH value. Cooling temperature rate of TK protocol was of 1 °C/min to −40 °C and 3 °C/min to −120 °C, and almost all of dimethyl sulfoxide left the tissue after washing. Future studies should be done changing cryopreservation-related variables of the TK protocol to store corneas of optical grade.es_ES
dc.description.sponsorshipThis research was funded by Xunta de Galicia (grant no. ED431B 2020/55), by Xunta de Galicia and the European Union (European Regional Development Fund; grant nos. ED481A-2019/206 and ED481A-2017/280), and by INDITEX and the University of A Coruña (grant no. 2019)es_ES
dc.description.sponsorshipXunta de Galicia; ED431B 2020/55es_ES
dc.description.sponsorshipXunta de Galicia; ED481A-2019/206es_ES
dc.description.sponsorshipXunta de Galicia; ED481A-2017/280
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation.urihttps://doi.org/10.3390/ijms222212564es_ES
dc.rightsCreative Commons Attribution 4.0 International Licence (CC-BY 4.0)es_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCorneal cryopreservationes_ES
dc.subjectCryoprotective agentes_ES
dc.subjectCorneal endotheliumes_ES
dc.subjectCorneal storagees_ES
dc.subjectCorneal transplantationes_ES
dc.subjectDMSOes_ES
dc.titleAnalysis of Cryopreservation Protocols and Their Harmful Effects on the Endothelial Integrity of Human Corneases_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleInternational Journal of Molecular Scienceses_ES
UDC.volume22es_ES
UDC.startPage12564es_ES
dc.identifier.doi10.3390/ijms222212564


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