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dc.contributor.authorFafián Labora, Juan Antonio
dc.contributor.authorO'Loghlen, Ana
dc.date.accessioned2021-09-27T09:11:37Z
dc.date.available2021-09-27T09:11:37Z
dc.date.issued2021-06-29
dc.identifier.citationFafián-Labora JA, O'Logjlen A. NF-κB/IKK activation by small extracellular vesicles within the SASP. Aging Cell. 2021;20(7):e13426es_ES
dc.identifier.issn1474-9718
dc.identifier.urihttp://hdl.handle.net/2183/28514
dc.description.abstract[Abstract] Cellular senescence plays an important role in different biological and pathological conditions. Senescent cells communicate with their microenvironment through a plethora of soluble factors, metalloproteases and extracellular vesicles (EV). Although much is known about the role that soluble factors play in senescence, the downstream signalling pathways activated by EV in senescence is unknown. To address this, we performed a small molecule inhibitor screen and have identified the IκB kinases IKKε, IKKα and IKKβ as essential for senescence mediated by EV (evSASP). By using pharmacological inhibitors of IKKε, IKKα and IKKβ, in addition to CRISPR/Cas9 targeting their respective genes, we find these pathways are important in mediating senescence. In addition, we find that senescence activation is dependent on canonical NF-κB transcription factors where siRNA targeting p65 prevent senescence. Importantly, these IKK pathways are also relevant to ageing as knockout of IKKA, IKKB and IKKE avoid the activation of senescence. Altogether, these findings open a new potential line of investigation in the field of senescence by targeting the negative effects of the evSASP independent of particular EV contents.es_ES
dc.description.sponsorshipAO’s lab is supported by BBSRC (BB/P000223/1) and Bart’s Charity Grant (MGU0497). J.A.F.-L is funded by the Xunta de Galicia Fellowship (ED481B 2017/117)es_ES
dc.description.sponsorshipXunta de Galicia; ED481B 2017/117
dc.description.sponsorshipReino Unido. Biotechnology and Biological Sciences Research Council; BB/P000223/1
dc.description.sponsorshipBarts Charity (Londres); MGU0497
dc.language.isoenges_ES
dc.publisherWileyes_ES
dc.relation.urihttps://doi.org/10.1111/acel.13426es_ES
dc.rightsCreative Commons Attribution 4.0 International License (CC-BY 4.0)es_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectIκB kinase (IKK)es_ES
dc.subjectNF-κBes_ES
dc.subjectSASPes_ES
dc.subjectAgeinges_ES
dc.subjectExtracellular vesicles (EV)es_ES
dc.subjectSenescencees_ES
dc.titleNF-κB/IKK Activation by Small Extracellular Vesicles Within the SASPes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleAging Celles_ES
UDC.volume20es_ES
UDC.issue7es_ES
UDC.startPagee13426es_ES
dc.identifier.doi10.1111/acel.13426


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