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dc.contributor.authorFafián Labora, Juan Antonio
dc.contributor.authorMorente-López, Miriam
dc.contributor.authorSánchez-Dopico, María José
dc.contributor.authorArntz, O.J.
dc.contributor.authorVan de Loo, F.J.
dc.contributor.authorDe-Toro, Javier
dc.contributor.authorArufe, M.C.
dc.date.accessioned2020-01-29T10:07:05Z
dc.date.available2020-01-29T10:07:05Z
dc.date.issued2020-01-03
dc.identifier.citationFafián-Labora J, Morente-López M, Sánchez-Dopico M.J. et al. Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their tole in ageing. Stem Cell Res Ther. 2020; 11:13es_ES
dc.identifier.issn1757-6512
dc.identifier.urihttp://hdl.handle.net/2183/24783
dc.description.abstract[Abstract] Introduction: This study assessed whether mesenchymal stem cell (MSC)-derived extracellular vesicles influenced ageing and pluripotency markers in cell cultures where they are added. Methods: MSC-derived extracellular vesicles from old and young rat bone marrows were isolated by ultracentrifugation and were characterised by western blotting, nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM). They were added to young and old MSC cultures. Real-time quantitative reverse transcription polymerase chain reactions and western blot analysis were performed to check the markers of ageing (vinculin and lamin A), pluripotency markers (Nanog and Oct4) and components of the mTOR signalling pathway (Rictor, Raptor, AKT and mTOR) in these cell populations. Subsequently, microRNA (miR)-188-3p expression was transiently inhibited in young MSCs to demonstrate the influence of mTOR2 on MSC ageing. Results: Incubation with young MSC-derived extracellular vesicles decreased the levels of ageing markers and components of the mTOR pathway and increased the pluripotency markers from old MSC populations. By contrast, incubation of young MSCs with old MSC-derived extracellular vesicles generated the reverse effects. Inhibition of miR-188-3p expression in young MSCs produced extracellular vesicles that when incubated with old MSCs produced an increase in the levels of Rictor, as well as a decrease of phosphor-AKT, as indicated by a significant decrease in beta-galactosidase staining. Conclusions: MSC-derived extracellular vesicles affected the behaviour of MSC cultures, based on their composition, which could be modified in vitro. These experiments represented the basis for the development of new therapies against ageing-associated diseases using MSC-derived extracellular vesicles.es_ES
dc.language.isoenges_ES
dc.publisherSpringer Naturees_ES
dc.relation.urihttps://doi.org/10.1186/s13287-019-1534-0es_ES
dc.rightsCreative Commons Attribution 4.0 International License (CC-BY 4.0)es_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMesenchymal stem cell-derived extracellular vesicleses_ES
dc.subjectAgeinges_ES
dc.subjectPluripotencyes_ES
dc.subjectmTOR pathwayes_ES
dc.titleInfluence of mesenchymal stem cell-derived extracellular vesicles in vitro and their tole in ageinges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleStem Cell Research & Therapyes_ES
UDC.volume11es_ES
UDC.startPage13es_ES
dc.identifier.doi10.1186/s13287-019-1534-0


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