Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their tole in ageing
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Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their tole in ageingAuthor(s)
Date
2020-01-03Citation
Fafián-Labora J, Morente-López M, Sánchez-Dopico M.J. et al. Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their tole in ageing. Stem Cell Res Ther. 2020; 11:13
Abstract
[Abstract]
Introduction: This study assessed whether mesenchymal stem cell (MSC)-derived extracellular vesicles influenced
ageing and pluripotency markers in cell cultures where they are added.
Methods: MSC-derived extracellular vesicles from old and young rat bone marrows were isolated by
ultracentrifugation and were characterised by western blotting, nanoparticle tracking analysis (NTA) and
transmission electron microscopy (TEM). They were added to young and old MSC cultures. Real-time quantitative
reverse transcription polymerase chain reactions and western blot analysis were performed to check the markers of
ageing (vinculin and lamin A), pluripotency markers (Nanog and Oct4) and components of the mTOR signalling
pathway (Rictor, Raptor, AKT and mTOR) in these cell populations. Subsequently, microRNA (miR)-188-3p expression
was transiently inhibited in young MSCs to demonstrate the influence of mTOR2 on MSC ageing.
Results: Incubation with young MSC-derived extracellular vesicles decreased the levels of ageing markers and
components of the mTOR pathway and increased the pluripotency markers from old MSC populations. By contrast,
incubation of young MSCs with old MSC-derived extracellular vesicles generated the reverse effects. Inhibition of
miR-188-3p expression in young MSCs produced extracellular vesicles that when incubated with old MSCs
produced an increase in the levels of Rictor, as well as a decrease of phosphor-AKT, as indicated by a significant
decrease in beta-galactosidase staining.
Conclusions: MSC-derived extracellular vesicles affected the behaviour of MSC cultures, based on their
composition, which could be modified in vitro. These experiments represented the basis for the development of
new therapies against ageing-associated diseases using MSC-derived extracellular vesicles.
Keywords
Mesenchymal stem cell-derived extracellular vesicles
Ageing
Pluripotency
mTOR pathway
Ageing
Pluripotency
mTOR pathway
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Creative Commons Attribution 4.0 International License (CC-BY 4.0)
ISSN
1757-6512