Conjugation of different immunogenic enterococcal vaccine target antigens leads to extended strain coverage
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Conjugation of different immunogenic enterococcal vaccine target antigens leads to extended strain coverageAuthor(s)
Date
2019-07-09Citation
F Romero-Saavedra, D Laverde, E Kalfopoulou, C Martini, R Torelli, D Martinez-Matamoros, M Sanguinetti, J Huebner, Conjugation of Different Immunogenic Enterococcal Vaccine Target Antigens Leads to Extended Strain Coverage, The Journal of Infectious Diseases, Volume 220, Issue 10, 15 November 2019, Pages 1589–1598, https://doi.org/10.1093/infdis/jiz357
Abstract
[Abstract] Enterococci have emerged as important nosocomial pathogens due to their resistance to the most commonly used antibiotics. Alternative treatments or prevention options are aimed at polysaccharides and surface-related proteins that play important roles in pathogenesis. Previously, we have shown that 2 Enterococcus faecium proteins, the secreted antigen A and the peptidyl-prolyl cis-trans isomerase, as well as the Enterococcus faecalis polysaccharide diheteroglycan, are able to induce opsonic and cross-protective antibodies. Here, we evaluate the use of glycoconjugates consisting of these proteins and an enterococcal polysaccharide to develop a vaccine with broader strain coverage. Diheteroglycan was conjugated to these 2 enterococcal proteins. Rabbit sera raised against these glycoconjugates showed Immunoglobulin G titers against the corresponding conjugate, as well as against the respective protein and carbohydrate antigens. Effective opsonophagocytic killing for the 2 sera was observed against different E. faecalis and E. faecium strains. Enzyme-linked immunosorbent assays against whole bacterial cells showed immune recognition of 22 enterococcal strains by the sera. Moreover, the sera conferred protection against E. faecalis and E. faecium strains in a mouse infection model. Our results suggest that these glycoconjugates are promising candidates for vaccine formulations with a broader coverage against these nosocomial pathogens and that the evaluated proteins are potential carrier proteins.
Keywords
Vaccine
Glycoconjugate
Carrier protein
Capsular polysaccharide
Diheteroglycan
Enterococcal proteins
Enterococcus faecalis
Enterococcus faecium
Opsonophagocytic assay
Mouse infection model
Glycoconjugate
Carrier protein
Capsular polysaccharide
Diheteroglycan
Enterococcal proteins
Enterococcus faecalis
Enterococcus faecium
Opsonophagocytic assay
Mouse infection model
Editor version
Rights
Atribución-NoComercial-SinDerivadas 3.0 España
ISSN
0022-1899
1537-6613
1537-6613