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Conjugation of different immunogenic enterococcal vaccine target antigens leads to extended strain coverage
dc.contributor.author | Romero-Saavedra, Felipe | |
dc.contributor.author | Laverde, Diana | |
dc.contributor.author | Kalfopoulou, Ermioni | |
dc.contributor.author | Martini, Cecilia | |
dc.contributor.author | Torelli, Riccardo | |
dc.contributor.author | Martínez Matamoros, Diana | |
dc.contributor.author | Sanguinetti, Maurizio | |
dc.contributor.author | Huebner, Johannes | |
dc.date.accessioned | 2019-11-05T09:55:36Z | |
dc.date.available | 2019-11-05T09:55:36Z | |
dc.date.issued | 2019-07-09 | |
dc.identifier.citation | F Romero-Saavedra, D Laverde, E Kalfopoulou, C Martini, R Torelli, D Martinez-Matamoros, M Sanguinetti, J Huebner, Conjugation of Different Immunogenic Enterococcal Vaccine Target Antigens Leads to Extended Strain Coverage, The Journal of Infectious Diseases, Volume 220, Issue 10, 15 November 2019, Pages 1589–1598, https://doi.org/10.1093/infdis/jiz357 | es_ES |
dc.identifier.issn | 0022-1899 | |
dc.identifier.issn | 1537-6613 | |
dc.identifier.uri | http://hdl.handle.net/2183/24230 | |
dc.description.abstract | [Abstract] Enterococci have emerged as important nosocomial pathogens due to their resistance to the most commonly used antibiotics. Alternative treatments or prevention options are aimed at polysaccharides and surface-related proteins that play important roles in pathogenesis. Previously, we have shown that 2 Enterococcus faecium proteins, the secreted antigen A and the peptidyl-prolyl cis-trans isomerase, as well as the Enterococcus faecalis polysaccharide diheteroglycan, are able to induce opsonic and cross-protective antibodies. Here, we evaluate the use of glycoconjugates consisting of these proteins and an enterococcal polysaccharide to develop a vaccine with broader strain coverage. Diheteroglycan was conjugated to these 2 enterococcal proteins. Rabbit sera raised against these glycoconjugates showed Immunoglobulin G titers against the corresponding conjugate, as well as against the respective protein and carbohydrate antigens. Effective opsonophagocytic killing for the 2 sera was observed against different E. faecalis and E. faecium strains. Enzyme-linked immunosorbent assays against whole bacterial cells showed immune recognition of 22 enterococcal strains by the sera. Moreover, the sera conferred protection against E. faecalis and E. faecium strains in a mouse infection model. Our results suggest that these glycoconjugates are promising candidates for vaccine formulations with a broader coverage against these nosocomial pathogens and that the evaluated proteins are potential carrier proteins. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/675671 | es_ES |
dc.relation.uri | https://doi.org/10.1093/infdis/jiz357 | es_ES |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Vaccine | es_ES |
dc.subject | Glycoconjugate | es_ES |
dc.subject | Carrier protein | es_ES |
dc.subject | Capsular polysaccharide | es_ES |
dc.subject | Diheteroglycan | es_ES |
dc.subject | Enterococcal proteins | es_ES |
dc.subject | Enterococcus faecalis | es_ES |
dc.subject | Enterococcus faecium | es_ES |
dc.subject | Opsonophagocytic assay | es_ES |
dc.subject | Mouse infection model | es_ES |
dc.title | Conjugation of different immunogenic enterococcal vaccine target antigens leads to extended strain coverage | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | The Journal of Infectious Diseases | es_ES |
UDC.volume | 220 | es_ES |
UDC.issue | 10 | es_ES |
UDC.startPage | 1589 | es_ES |
UDC.endPage | 1598 | es_ES |
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