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dc.contributor.authorDelgado, Elena
dc.contributor.authorBenito, Sonia
dc.contributor.authorMontero, Vanessa
dc.contributor.authorCuevas, María Teresa
dc.contributor.authorFernández-García, Aurora
dc.contributor.authorSánchez-Martínez, Mónica
dc.contributor.authorGarcía-Bodas, Elena
dc.contributor.authorDíez-Fuertes, Francisco
dc.contributor.authorGil, Horacio
dc.contributor.authorCañada, Javier
dc.contributor.authorCarrera, Cristina
dc.contributor.authorMartínez-López, Jesús
dc.contributor.authorSintes, Marcos
dc.contributor.authorPérez-Álvarez, Lucía
dc.contributor.authorThomson, Michael M.
dc.contributor.authorCastro-Iglesias, Ángeles
dc.date.accessioned2019-09-13T08:35:39Z
dc.date.available2019-09-13T08:35:39Z
dc.date.issued2019-08-03
dc.identifier.citationDelgado E, Benito S, Montero V, et al. Diverse large HIV-1 non-subtype B clusters are spreading among men who have sex with men in Spain. Front Microbiol. 2019; 10:655.es_ES
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/2183/23929
dc.descriptionOriginal researches_ES
dc.description.abstract[Abstract] In Western Europe, the HIV-1 epidemic among men who have sex with men (MSM) is dominated by subtype B. However, recently, other genetic forms have been reported to circulate in this population, as evidenced by their grouping in clusters predominantly comprising European individuals. Here we describe four large HIV-1 non-subtype B clusters spreading among MSM in Spain. Samples were collected in 9 regions. A pol fragment was amplified from plasma RNA or blood-extracted DNA. Phylogenetic analyses were performed via maximum likelihood, including database sequences of the same genetic forms as the identified clusters. Times and locations of the most recent common ancestors (MRCA) of clusters were estimated with a Bayesian method. Five large non-subtype B clusters associated with MSM were identified. The largest one, of F1 subtype, was reported previously. The other four were of CRF02_AG (CRF02_1; n = 115) and subtypes A1 (A1_1; n = 66), F1 (F1_3; n = 36), and C (C_7; n = 17). Most individuals belonging to them had been diagnosed of HIV-1 infection in the last 10 years. Each cluster comprised viruses from 3 to 8 Spanish regions and also comprised or was related to viruses from other countries: CRF02_1 comprised a Japanese subcluster and viruses from 8 other countries from Western Europe, Asia, and South America; A1_1 comprised viruses from Portugal, United Kingom, and United States, and was related to the A1 strain circulating in Greece, Albania and Cyprus; F1_3 was related to viruses from Romania; and C_7 comprised viruses from Portugal and was related to a virus from Mozambique. A subcluster within CRF02_1 was associated with heterosexual transmission. Near full-length genomes of each cluster were of uniform genetic form. Times of MRCAs of CRF02_1, A1_1, F1_3, and C_7 were estimated around 1986, 1989, 2013, and 1983, respectively. MRCA locations for CRF02_1 and A1_1 were uncertain (however initial expansions in Spain in Madrid and Vigo, respectively, were estimated) and were most probable in Bilbao, Spain, for F1_3 and Portugal for C_7. These results show that the HIV-1 epidemic among MSM in Spain is becoming increasingly diverse through the expansion of diverse non-subtype B clusters, comprising or related to viruses circulating in other countries.es_ES
dc.description.sponsorshipInstituto de Salud Carlos III; PI16CIII/00033es_ES
dc.description.sponsorshipInstituto de Salud Carlos III; RD16ISCIII/0002/0004es_ES
dc.description.sponsorshipXunta de Galicia; MVI 1004/16es_ES
dc.description.sponsorshipGobierno Vasco; MVI 1001/16es_ES
dc.description.sponsorshipMinisterio de Sanidad, Servicios Sociales e Igualdad (España); EC11-272es_ES
dc.language.isoenges_ES
dc.publisherFrontierses_ES
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/312661es_ES
dc.relation.urihttps://doi.org/10.3389/fmicb.2019.00655es_ES
dc.rightsAtribución 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectHIV-1es_ES
dc.subjectMolecular epidemiologyes_ES
dc.subjectPhylogenyes_ES
dc.subjectPhylodynamicses_ES
dc.subjectMen who have sex with menes_ES
dc.subjectSubtypeses_ES
dc.subjectCirculating recombinant formses_ES
dc.subjectClusterses_ES
dc.titleDiverse large HIV-1 non-subtype B clusters are spreading among men who have sex with men in Spaines_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleFrontiers in Microbiologyes_ES
UDC.volume10es_ES
UDC.startPage655es_ES


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