Show simple item record

dc.contributor.authorFafián Labora, Juan Antonio
dc.contributor.authorLesende-Rodríguez, Iván
dc.contributor.authorFernández-Pernas, Pablo
dc.contributor.authorSangiao-Alvarellos, Susana
dc.contributor.authorMonserrat, Lorenzo
dc.contributor.authorArntz, O.J.
dc.contributor.authorVan de Loo, F.J.
dc.contributor.authorMateos, Jesús
dc.contributor.authorArufe, M.C.
dc.date.accessioned2017-08-31T09:01:54Z
dc.date.available2017-08-31T09:01:54Z
dc.date.issued2017-03-06
dc.identifier.citationFafián-Labora J, Lesende-Rodriguez I, Fernández-Pernas P, Sangiao-Alvarellos S, Monserrat L, Arntz OJ, van de Loo FJ, Mateos J, Arufe MC. Effect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicles. Sci Rep. 2017 Mar 6;7:43923.es_ES
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/2183/19412
dc.description.abstract[Abstract] Stem cells possess significant age-dependent differences in their immune-response profile. These differences were analysed by Next-Generation Sequencing of six age groups from bone marrow mesenchymal stem cells. A total of 9,628 genes presenting differential expression between age groups were grouped into metabolic pathways. We focused our research on young, pre-pubertal and adult groups, which presented the highest amount of differentially expressed genes related to inflammation mediated by chemokine and cytokine signalling pathways compared with the newborn group, which was used as a control. Extracellular vesicles extracted from each group were characterized by nanoparticle tracking and flow cytometry analysis, and several micro-RNAs were verified by quantitative real-time polymerase chain reaction because of their relationship with the pathway of interest. Since miR-21-5p showed the highest statistically significant expression in extracellular vesicles from mesenchymal stem cells of the pre-pubertal group, we conducted a functional experiment inhibiting its expression and investigating the modulation of Toll-Like Receptor 4 and their link to damage-associated molecular patterns. Together, these results indicate for the first time that mesenchymal stem cell-derived extracellular vesicles have significant age-dependent differences in their immune profiles.es_ES
dc.description.sponsorshipThis study was supported by a grant from Instituto de Salud Carlos III - Ministerio Economía y Competitividad No. Expediente PI11/02799 Unión Europea - Fondo Europeo de Desarrollo Regional (FEDER) “Una manera de hacer Europa”. J.F.-L. was supported by the European Molecular Biology Organization (EMBO) during a short-term fellowship in Van de Loo’s group. S.S.-A. was supported by a grant EM2013/011 (Xunta de Galicia, Spain).es_ES
dc.description.sponsorshipXunta de Galicia; EM2013/011
dc.language.isoenges_ES
dc.publisherNaturees_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN/Plan nacional de I+D+i 2008-2011/PI11%2F02799/ES/Combinación de terapias celulares para la reparación de lesiones de cartílago
dc.relation.urihttp://dx.doi.org/10.1038/srep43923es_ES
dc.rightsCreative Commons Attribution 4.0 International License (CC-BY 4.0)es_ES
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.titleEffect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicleses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessinfo:eu-repo/semantics/openAccesses_ES
UDC.journalTitleScientific Reportses_ES
UDC.volume7es_ES
UDC.startPage43923es_ES


Files in this item

Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record