Ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineering

Ver/Abrir
Use este enlace para citar
http://hdl.handle.net/2183/18118
Excepto si se señala otra cosa, la licencia del ítem se describe como Creative Commons Attribution 4.0 International Licence (CC-BY 4.0)
Colecciones
- Investigación (FCS) [1277]
Metadatos
Mostrar el registro completo del ítemTítulo
Ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineeringAutor(es)
Fecha
2017-01-31Cita bibliográfica
Sanjurjo-Rodríguez C, Castro-Viñuelas R, Hermida-Gómez T, Fernández-Vázquez T, Fuentes-Boquete IM, de Toro-Santos FJ, Díaz-Prado SM, Blanco-García FJ. Ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineering. PLoS One. 2017 Jan 31;12(1):e0171231.
Resumen
[Abstract] Introduction. Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral diseases.
Purpose. The aim was to isolate and characterize oMSCs from bone marrow aspirates (oBMSCs) and to assess their usefulness for osteochondral repair using β-tricalcium phosphate (bTCP) and type I collagen (Col I) scaffolds.
Methods. Cells isolated from ovine bone marrow were characterized morphologically, phenotypically, and functionally. oBMSCs were cultured with osteogenic medium on bTCP and Col I scaffolds. The resulting constructs were evaluated by histology, immunohistochemistry and electron microscopy studies. Furthermore, oBMSCs were cultured on Col I scaffolds to develop an in vitro cartilage repair model that was assessed using a modified International Cartilage Research Society (ICRS) II scale.
Results. oBMSCs presented morphology, surface marker pattern and multipotent capacities similar to those of human BMSCs. oBMSCs seeded on Col I gave rise to osteogenic neotissue. Assessment by the modified ICRS II scale revealed that fibrocartilage/hyaline cartilage was obtained in the in vitro repair model.
Conclusions. The isolated ovine cells were demonstrated to be oBMSCs. oBMSCs cultured on Col I sponges successfully synthesized osteochondral tissue. The data suggest that oBMSCs have potential for use in preclinical models prior to human clinical studies.
Versión del editor
Derechos
Creative Commons Attribution 4.0 International Licence (CC-BY 4.0)
ISSN
1932-6203