Listar GI-EXPRELA - Artigos por autor "González-Siso, María-Isabel"

Mostrando ítems 1-17 de 17

    • Biochemical and structural characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family 

      Miguel-Ruano, Vega; Rivera, Ivanna; Rajkovic, Jelena; Kamila, Knapik; Torrado Agrasar, Ana; Otero, J. M.; Beneventi, Elisa; Becerra, Manuel; Sánchez-Costa, Mercedes; Hidalgo, Aurelio; Berenguer, José; González-Siso, María-Isabel; Cruces, Jacobo; Rúa Rodríguez, María Luisa; Hermoso, Juan (Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology, 2021)
      [Abstract]: A novel esterase, EstD11, has been discovered in a hot spring metagenomic library. It is a thermophilic and thermostable esterase with an optimum temperature of 60 C. A detailed substrate preference analysis ...

    • Bioprospecting for Thermozymes and Characterization of a Novel Lipolytic Thermozyme Belonging to the SGNH/GDSL Family of Hydrolases 

      Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Saavedra-Bouza, Almudena; González-Siso, María-Isabel; Becerra, Manuel (MDPI, 2022-05-20)
      [Abstract] Functional screenings were conducted on two metagenomic libraries from hot springs in order to find novel thermozymes with potential biotechnological applications. These included enzymes acting on plant cell ...

    • Cellulases from thermophiles found by metagenomics 

      Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Cerdán, María Esperanza; Rodríguez-Belmonte, Esther; Becerra, Manuel; González-Siso, María-Isabel (MDPI, 2018-07-10)
      [Abstract] Cellulases are a heterogeneous group of enzymes that synergistically catalyze the hydrolysis of cellulose, the major component of plant biomass. Such reaction has biotechnological applications in a broad spectrum ...

    • Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide 

      De Castro de Antonio, María Eugenia; Escuder-Rodríguez, Juan-José; Becerra, Manuel; Rodríguez-Belmonte, Esther; González-Siso, María-Isabel (Frontiers, 2021-11-26)
      [Abstract] With their circumneutral pH and their moderate temperature (66 and 68°C, respectively), As Burgas and Muiño da Veiga are two important human-use hot springs, previously studied with traditional culture methods, ...

    • Editorial for the special issue: Thermophiles and Thermozymes 

      González-Siso, María-Isabel (MDPI, 2019-02-27)
      [Abstract:] Heat-loving microorganisms or thermophiles arouse noticeable scientific interest nowadays, not only with the aim to elucidate the mystery of life at high temperatures, but also due to the huge field of ...

    • Exploring the Taxonomical and Functional Profile of As Burgas Hot Spring Focusing On Thermostable β-galactosidases 

      De Castro de Antonio, María Eugenia; Doane, Michael P.; Dinsdale, Elizabeth Ann; Rodríguez-Belmonte, Esther; González-Siso, María-Isabel (Springer Nature, 2021-01-08)
      [Abstract] In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate ...

    • Hot Spring Metagenomics 

      López-López, Olalla; Cerdán, María Esperanza; González-Siso, María-Isabel (Multidisciplinary Digital Publishing Institute, 2013)
      Hot springs have been investigated since the XIX century, but isolation and examination of their thermophilic microbial inhabitants did not start until the 1950s. Many thermophilic microorganisms and their viruses have ...

    • Insights on Microbial Communities Inhabiting Non-Volcanic Hot Springs 

      Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Saavedra-Bouza, Almudena; Becerra, Manuel; González-Siso, María-Isabel (MDPI, 2022-10-13)
      [Abstract] The northwest of Spain has an abundance of non-volcanic hot springs that, until recently, had only been used for thermalism activities. One of such hot springs, Muiño da Veiga, has now been explored using ...

    • Ixr1 Regulates Ribosomal Gene Transcription and Yeast Response to Cisplatin 

      Vizoso-Vázquez, Ángel; Lamas, Mónica; González-Siso, María-Isabel; Cerdán, María Esperanza (Springer Nature, 2018-02-15)
      [Abstract] Ixr1 is a Saccharomyces cerevisiae HMGB protein that regulates the hypoxic regulon and also controls the expression of other genes involved in the oxidative stress response or re-adaptation of catabolic and ...

    • Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain 

      Knapik, Kamila; Becerra, Manuel; González-Siso, María-Isabel (Springer Nature, 2019-08-01)
      [Abstract] Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases. ...

    • Novel Microbial Enzymes with Industrial Applications [Editorial] 

      González-Siso, María-Isabel; Becerra, Manuel (MDPI, 2023-04-10)

    • Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides 

      Álvarez Cao, María E.; Cerdán, María Esperanza; González-Siso, María-Isabel; Becerra, Manuel (BMC, 2019-10-10)
      [Abstract] Background: α-Galactosidases are enzymes that act on galactosides present in many vegetables, mainly legumes and cereals, have growing importance with respect to our diet. For this reason, the use of their ...

    • Production and characterization of an extracellular acid protease from thermophilic Brevibacillus sp. OA30 isolated from an Algerian hot spring 

      Gomri, Mohamed Amine; Rico Díaz, Agustín; Escuder-Rodríguez, Juan-José; El Moulouk Khaldi, Tedj; González-Siso, María-Isabel; Kharroub, Karima (MDPI, 2018-04-12)
      [Abstract] Proteases have numerous biotechnological applications and the bioprospection for newly-thermostable proteases from the great biodiversity of thermophilic microorganisms inhabiting hot environments, such as ...

    • Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin 

      Rodríguez Lombardero, Silvia; Rodríguez-Belmonte, Esther; González-Siso, María-Isabel; Vizoso-Vázquez, Ángel; Valdiglesias, Vanessa; Laffon, Blanca; Cerdán, María Esperanza (Multidisciplinary Digital Publishing Institute, 2014)
      [Abstract] Sky1 is the only member of the SR (Serine–Arginine) protein kinase family in Saccharomyces cerevisiae. When yeast cells are treated with the anti-cancer drug cisplatin, Sky1 kinase activity is necessary to produce ...

    • Reactivity of a Recombinant Esterase from Thermus thermophilus HB27 in Aqueous and Organic Media 

      González González, Roberto; Fuciños, Pablo; Beneventi, Elisa; López-López, Olalla; Pampín, Begoña; Rodríguez, Ramón; González-Siso, María-Isabel; Cruces, Jacobo; Rúa Rodríguez, María Luisa (MDPI, 2022-04-27)
      [Abstract] The thermoalkalophilic membrane-associated esterase E34Tt from Thermus thermophilus HB27 was cloned and expressed in Kluyveromyces lactis (KLEST-3S esterase). The recombinant enzyme was tested as a biocatalyst ...

    • Thermus thermophilus as a Source of Thermostable Lipolytic Enzymes 

      López-López, Olalla; Cerdán, María Esperanza; González-Siso, María-Isabel (Multidisciplinary Digital Publishing Institute, 2015)
      Lipolytic enzymes, esterases (EC 3.1.1.1) and lipases (EC 3.1.1.3), catalyze the hydrolysis of ester bonds between alcohols and carboxylic acids, and its formation in organic media. At present, they represent about 20% of ...

    • Xylanases From Thermophilic Archaea: A Hidden Treasure 

      Saavedra-Bouza, Almudena; Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Becerra, Manuel; González-Siso, María-Isabel (Elsevier, 2022-12-12)
      [Abstract] Archaea are a domain of prokaryotic organisms that often inhabit extremophilic environments. Their enzymes are therefore of enormous biotechnological interest, due to their stability and activity in harsh ...