Listar GI-EXPRELA - Artigos por fecha de publicación
Mostrando ítems 1-20 de 28
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Hot Spring Metagenomics
(Multidisciplinary Digital Publishing Institute, 2013)Hot springs have been investigated since the XIX century, but isolation and examination of their thermophilic microbial inhabitants did not start until the 1950s. Many thermophilic microorganisms and their viruses have ... -
Proteomic Analyses Reveal that Sky1 Modulates Apoptosis and Mitophagy in Saccharomyces cerevisiae Cells Exposed to Cisplatin
(Multidisciplinary Digital Publishing Institute, 2014)[Abstract] Sky1 is the only member of the SR (Serine–Arginine) protein kinase family in Saccharomyces cerevisiae. When yeast cells are treated with the anti-cancer drug cisplatin, Sky1 kinase activity is necessary to produce ... -
Thermus thermophilus as a Source of Thermostable Lipolytic Enzymes
(Multidisciplinary Digital Publishing Institute, 2015)Lipolytic enzymes, esterases (EC 3.1.1.1) and lipases (EC 3.1.1.3), catalyze the hydrolysis of ester bonds between alcohols and carboxylic acids, and its formation in organic media. At present, they represent about 20% of ... -
Ixr1 Regulates Ribosomal Gene Transcription and Yeast Response to Cisplatin
(Springer Nature, 2018-02-15)[Abstract] Ixr1 is a Saccharomyces cerevisiae HMGB protein that regulates the hypoxic regulon and also controls the expression of other genes involved in the oxidative stress response or re-adaptation of catabolic and ... -
Delineating the HMGB1 and HMGB2 Interactome in Prostate and Ovary Epithelial Cells and Its Relationship With Cancer
(Impact Journals, 2018-04-10)[Abstract] High Mobility Group B (HMGB) proteins are involved in cancer progression and in cellular responses to platinum compounds used in the chemotherapy of prostate and ovary cancer. Here we use affinity purification ... -
Production and characterization of an extracellular acid protease from thermophilic Brevibacillus sp. OA30 isolated from an Algerian hot spring
(MDPI, 2018-04-12)[Abstract] Proteases have numerous biotechnological applications and the bioprospection for newly-thermostable proteases from the great biodiversity of thermophilic microorganisms inhabiting hot environments, such as ... -
Cellulases from thermophiles found by metagenomics
(MDPI, 2018-07-10)[Abstract] Cellulases are a heterogeneous group of enzymes that synergistically catalyze the hydrolysis of cellulose, the major component of plant biomass. Such reaction has biotechnological applications in a broad spectrum ... -
Editorial for the special issue: Thermophiles and Thermozymes
(MDPI, 2019-02-27)[Abstract:] Heat-loving microorganisms or thermophiles arouse noticeable scientific interest nowadays, not only with the aim to elucidate the mystery of life at high temperatures, but also due to the huge field of ... -
Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain
(Springer Nature, 2019-08-01)[Abstract] Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases. ... -
Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides
(BMC, 2019-10-10)[Abstract] Background: α-Galactosidases are enzymes that act on galactosides present in many vegetables, mainly legumes and cereals, have growing importance with respect to our diet. For this reason, the use of their ... -
Characterization of HMGB1/2 Interactome in Prostate Cancer by Yeast Two Hybrid Approach: Potential Pathobiological Implications
(MDPI, 2019-11-05)[Abstract] High mobility group box B (HMGB) proteins are pivotal in the development of cancer. Although the proteomics of prostate cancer (PCa) cells has been reported, the involvement of HMGB proteins and their interactome ... -
The Challenges and Opportunities of lncRNAs in Ovarian Cancer Research and Clinical Use
(MDPI, 2020-04-21)[Abstract] Ovarian cancer is one of the most lethal gynecological malignancies worldwide because it tends to be detected late, when the disease has already spread, and prognosis is poor. In this review we aim to highlight ... -
The HMGB1-2 Ovarian Cancer Interactome: the Role of HMGB Proteins and Their Interacting Partners MIEN1 and NOP53 in Ovary Cancer and Drug-Response
(MDPI, 2020-08-27)[Abstract] High mobility group box B (HMGB) proteins are overexpressed in different types of cancers such as epithelial ovarian cancers (EOC). We have determined the first interactome of HMGB1 and HMGB2 in epithelial ovarian ... -
Biochemical and structural characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family
(Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology, 2021)[Abstract]: A novel esterase, EstD11, has been discovered in a hot spring metagenomic library. It is a thermophilic and thermostable esterase with an optimum temperature of 60 C. A detailed substrate preference analysis ... -
Exploring the Taxonomical and Functional Profile of As Burgas Hot Spring Focusing On Thermostable β-galactosidases
(Springer Nature, 2021-01-08)[Abstract] In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate ... -
The HMGB Protein KlIxr1, a DNA Binding Regulator of Kluyveromyces lactis Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis
(MDPI, 2021-08-21)[Abstract] In the traditional fermentative model yeast Saccharomyces cerevisiae, ScIxr1 is an HMGB (High Mobility Group box B) protein that has been considered as an important regulator of gene transcription in response ... -
HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes
(MDPI, 2021-09-18)[Abstract] This study reports the HMGB1 interactomes in prostate and ovary cancer cells lines. Affinity purification coupled to mass spectrometry confirmed that the HMGB1 nuclear interactome is involved in HMGB1 known ... -
Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide
(Frontiers, 2021-11-26)[Abstract] With their circumneutral pH and their moderate temperature (66 and 68°C, respectively), As Burgas and Muiño da Veiga are two important human-use hot springs, previously studied with traditional culture methods, ... -
HMG Proteins from Molecules to Disease [Editorial]
(MDPI, 2022-02-17) -
Reactivity of a Recombinant Esterase from Thermus thermophilus HB27 in Aqueous and Organic Media
(MDPI, 2022-04-27)[Abstract] The thermoalkalophilic membrane-associated esterase E34Tt from Thermus thermophilus HB27 was cloned and expressed in Kluyveromyces lactis (KLEST-3S esterase). The recombinant enzyme was tested as a biocatalyst ...