Now showing items 1-20 of 23

    • Analysis of Cryopreservation Protocols and Their Harmful Effects on the Endothelial Integrity of Human Corneas 

      Rodríguez-Fernández, Silvia; Álvarez-Portela, Marcelino; Rendal Vázquez, María Esther; Piñeiro-Ramil, María; Sanjurjo-Rodríguez, Clara; Castro Viñuelas, Rocío; Sánchez-Ibáñez, Jacinto; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (MDPI, 2021-11-22)
      [Abstract] Corneal cryopreservation can partially solve the worldwide concern regarding donor cornea shortage for keratoplasties. In this study, human corneas were cryopreserved using two standard cryopreservation protocols ...
    • Cell therapy and tissue engineering for cartilage repair 

      Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (IntechOpen, 2018-02-14)
      [Abstract] The integrity of the articular cartilage is necessary for the proper functioning of the diarthrodial joint. The self-repair capacity of this tissue is very limited and, currently, there is no effective treatment ...
    • Cell viability assay in corneal endothelium 

      Rodríguez-Fernández, Silvia; Álvarez Portela, Marcelino; Rendal Vázquez, María Esther; Montero Salinas, Alejandro; Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Rojas, María Victoria de; Sánchez Ibáñez, Jacinto; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (2018)
      [Abstract] Introduction: Endothelium is the inner layer of the cornea, which must be viable for transplanting. The limited availability of corneas makes necessary the developing of preservation ...
    • Células madre pluripotentes inducidas: generación, caracterización y aplicaciones en el estudio y tratamiento de la artrosis 

      Castro Viñuelas, Rocío (2019)
      [Resumen] La artrosis es la patología articular de mayor prevalencia y una causa importante del deterioro de la calidad de vida. Sin embargo, no existen tratamientos efectivos ni fármacos que promuevan la reparación de ...
    • Corneal cryopreservation: and endothelial cell viability and histomorphological study of cornea 

      Rodríguez-Fernández, Silvia; Álvarez Portela, Marcelino; Rendal Vázquez, María Esther; Montero Salinas, Alejandro; Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Rojas, María Victoria de; Sánchez Ibáñez, Jacinto; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (2019)
    • Criopreservación de córnea: estudio de la viabilidad celular endotelial y de la histomofología de la córnea 

      Rodríguez-Fernández, Silvia; Álvarez Portela, Marcelino; Rendal Vázquez, María Esther; Montero Salinas, Alejandro; Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Rojas, María Victoria de; Sánchez Ibáñez, Jacinto; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (2019)
    • Current Development of Alternative Treatments for Endothelial Decompensation: Cell-Based Therapy 

      Rodríguez-Fernández, Silvia; Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Álvarez Portela, Marcelino; Fuentes Boquete, Isaac Manuel; Rendal Vázquez, María Esther; Díaz-Prado, Silvia (Elsevier, 2021-04-01)
      [Abstract] Current treatment for corneal endothelial dysfunction consists in the replacement of corneal endothelium by keratoplasty. Owing to the scarcity of donor corneas and the increasing number of transplants, alternative ...
    • Detección de la viabilidad celular sobre endotelio corneal 

      Rodríguez-Fernández, Silvia; Álvarez Portela, Marcelino; Rendal Vázquez, María Esther; Montero Salinas, Alejandro; Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Rojas, María Victoria de; Sánchez Ibáñez, Jacinto; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (2018)
    • Establishment of human induced pluripotent stem cell-lines (IPSC) for in vitro modelling hand ostheoarthritis. 

      Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (BMJ Journals, 2019-05-27)
    • Generation and Characterization of Human Induced Pluripotent Stem Cells (iPSCs) From Hand Osteoarthritis Patient-Derived Fibroblasts 

      Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Hermida Gómez, Tamara; Rodríguez-Fernández, Silvia; Oreiro, Natividad; De-Toro, Javier; Fuentes Boquete, Isaac Manuel; Blanco García, Francisco J; Díaz-Prado, Silvia (Nature Research, 2020-03-06)
      [Abstract] Knowledge and research results about hand osteoarthritis (hOA) are limited due to the lack of samples and animal models of the disease. Here, we report the generation of two induced pluripotent stem cell ...
    • Generation of a human control iPS cell line (ESi080‐A) from a donor with no rheumatic diseases 

      Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Rodríguez-Fernández, Silvia; Fuentes Boquete, Isaac Manuel; Blanco García, Francisco J; Díaz-Prado, Silvia (Elsevier, 2020-03)
      [Abstract] Here, we report the establishment of the human iPS cell line N1-FiPS4F#7 generated from skin cells of a patient with no rheumatic diseases, thus obtaining an appropriate control iPS cell line for researchers ...
    • Generation of mesenchymal cell lines derived from aged donors 

      Piñeiro-Ramil, María; Sanjurjo-Rodríguez, Clara; Rodríguez-Fernández, Silvia; Castro Viñuelas, Rocío; Hermida Gómez, Tamara; Blanco García, Francisco J; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (MDPI, 2021-10-01)
      [Abstract] Background: Mesenchymal stromal cells (MSCs) have the capacity for self-renewal and multi-differentiation, and for this reason they are considered a potential cellular source in regenerative medicine of cartilage ...
    • Generation of osteoarthritic mesenchymal stromal cell lines. 

      Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Rodríguez-Fernández, Silvia; Hermida Gómez, Tamara; De-Toro, Javier; Blanco García, Francisco J; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (BMJ Journals, 2019-05-27)
    • Generation of osteoarthritis and healthy mesenchymal cell lines for research on regenerative medicine for osteoarthritis 

      Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (2018-04)
      [Purpose] Bone-marrow mesenchymal stem cells (BM-MSCs) are multipotent self-renewal adult cells with potential to regenerate the damaged tissues in degenerative diseases such as osteoarthritis (OA). Nevertheless, research ...
    • Human cartilage engineering in an in vitro repair model using collagen scaffolds and mesenchymal stromal cells 

      Sanjurjo-Rodríguez, Clara; Castro Viñuelas, Rocío; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (Ivyspring, 2017-09-28)
      [Abstract] The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. Lesions were made ...
    • Immortalizing mesenchymal stromal cells from aged donors while keeping their essential features 

      Piñeiro-Ramil, María; Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Rodríguez-Fernández, Silvia; Hermida Gómez, Tamara; Blanco García, Francisco J; Fuentes Boquete, Isaac Manuel; Díaz-Prado, Silvia (Hindawi, 2020-06-16)
      [Abstract] Human bone marrow-derived mesenchymal stromal cells (MSCs) obtained from aged patients are prone to senesce and diminish their differentiation potential, therefore limiting their usefulness for osteochondral ...
    • Induced pluripotent stem cells for cartilage repair: current status and future perspectives 

      Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (AO Research Institute Davos, 2018-09-11)
      [Abstract] The establishment of cartilage regenerative medicine is an important clinical issue, but the search for cell sources able to restore cartilage integrity proves to be challenging. Human mesenchymal stromal cells ...
    • Ovine mesenchymal stromal cells for osteochondral tissue engineering 

      Sanjurjo-Rodríguez, Clara; Castro Viñuelas, Rocío; Hermida Gómez, Tamara; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Blanco García, Francisco J; Díaz-Prado, Silvia (Elsevier, 2017-04)
    • Ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineering 

      Sanjurjo-Rodríguez, Clara; Castro Viñuelas, Rocío; Hermida Gómez, Tamara; Fernández Vázquez, Tania; Fuentes Boquete, Isaac Manuel; De-Toro, Javier; Díaz-Prado, Silvia; Blanco García, Francisco J (PLoS ONE, 2017-01-31)
      [Abstract] Introduction. Knowledge of ovine mesenchymal stromal cells (oMSCs) is currently expanding. Tissue engineering combining scaffolding with oMSCs provides promising therapies for the treatment of osteochondral ...
    • Tips and Tricks for Successfully Culturing and Adapting Human Induced Pluripotent Stem Cells [Protocol] 

      Castro Viñuelas, Rocío; Sanjurjo-Rodríguez, Clara; Piñeiro-Ramil, María; Rodríguez-Fernández, Silvia; López-Baltar, Isidoro; Fuentes Boquete, Isaac Manuel; Blanco García, Francisco J; Díaz-Prado, Silvia (Cell Press, 2021-12)
      [Abstract] Reprogramming somatic cells toward pluripotency became possible over a decade ago. Since then, induced pluripotent stem cells (iPSCs) have served as a versatile and powerful tool not only for basic research but ...