Cell viability assay in corneal endothelium
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Cell viability assay in corneal endotheliumAutor(es)
Data
2018Cita bibliográfica
Rodríguez-Fernández S, Álvarez-Portela M, Rendal-Vázquez ME, et al. Cell viability assay in corneal endothelium. Póster presentado en: II Annual Meeting CINBIO; 2018 Jun 25-25; Vigo, España
Resumo
[Abstract] Introduction: Endothelium is the inner layer of the cornea, which must be viable for transplanting. The limited availability of corneas makes necessary the developing of preservation techniques that allow a long storage without losing endothelial viability.Objectives: Optimization of a cell viability assay in preserved corneas.Methods: One half of an endothelium from a cornea that was storage in hypothermic conditions and an endothelium of a cryopreserved cornea were stained with LIVE/DEAD imaging kit and Hoechst. The other half of endothelium was the negative control. Corneal endothelia were imaged using a fluorescence microscope.Results: Four sort of cells were visualized on both endothelia: viable cells with high esterase activity, intermediate cells with low esterase activity, non-viable cells without esterase activity, and cells only stained by Hoechst. Conclusions: Triple stain is effectiveto detect different sort of cells in endothelium of preserved corneas, included viable cells, depending on their esterase enzymatic activity and on cell and nuclear membrane damage.
Descrición
Resumen del póster publicado en el II Annual Meeting CINBIO abstracts book [Internet], p. 65