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Cell viability assay in corneal endothelium
dc.contributor.author | Rodríguez-Fernández, Silvia | |
dc.contributor.author | Álvarez Portela, Marcelino | |
dc.contributor.author | Rendal Vázquez, María Esther | |
dc.contributor.author | Montero Salinas, Alejandro | |
dc.contributor.author | Piñeiro-Ramil, María | |
dc.contributor.author | Castro Viñuelas, Rocío | |
dc.contributor.author | Rojas, María Victoria de | |
dc.contributor.author | Sánchez Ibáñez, Jacinto | |
dc.contributor.author | Fuentes Boquete, Isaac Manuel | |
dc.contributor.author | Díaz-Prado, Silvia | |
dc.date.accessioned | 2019-03-13T09:14:11Z | |
dc.date.available | 2019-03-13T09:14:11Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Rodríguez-Fernández S, Álvarez-Portela M, Rendal-Vázquez ME, et al. Cell viability assay in corneal endothelium. Póster presentado en: II Annual Meeting CINBIO; 2018 Jun 25-25; Vigo, España | es_ES |
dc.identifier.uri | http://hdl.handle.net/2183/22204 | |
dc.description | Resumen del póster publicado en el II Annual Meeting CINBIO abstracts book [Internet], p. 65 | es_ES |
dc.description.abstract | [Abstract] Introduction: Endothelium is the inner layer of the cornea, which must be viable for transplanting. The limited availability of corneas makes necessary the developing of preservation techniques that allow a long storage without losing endothelial viability.Objectives: Optimization of a cell viability assay in preserved corneas.Methods: One half of an endothelium from a cornea that was storage in hypothermic conditions and an endothelium of a cryopreserved cornea were stained with LIVE/DEAD imaging kit and Hoechst. The other half of endothelium was the negative control. Corneal endothelia were imaged using a fluorescence microscope.Results: Four sort of cells were visualized on both endothelia: viable cells with high esterase activity, intermediate cells with low esterase activity, non-viable cells without esterase activity, and cells only stained by Hoechst. Conclusions: Triple stain is effectiveto detect different sort of cells in endothelium of preserved corneas, included viable cells, depending on their esterase enzymatic activity and on cell and nuclear membrane damage. | es_ES |
dc.language.iso | eng | es_ES |
dc.title | Cell viability assay in corneal endothelium | es_ES |
dc.type | info:eu-repo/semantics/conferenceObject | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.conferenceTitle | II Annual Meeting CINBIO | es_ES |