Parvimonas micra can translocate from the subgingival sulcus of the human oral cavity to colorectal adenocarcinoma
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Parvimonas micra can translocate from the subgingival sulcus of the human oral cavity to colorectal adenocarcinomaAuthor(s)
Date
2023Citation
Conde-Pérez, K., Buetas, E., Aja-Macaya, P., Martin-De Arribas, E., Iglesias-Corrás, I., Trigo-Tasende, N., Nasser-Ali, M., Estévez, L. S., Rumbo-Feal, S., Otero-Alén, B., Noguera, J. F., Concha, Á., Pardiñas-López, S., Carda-Diéguez, M., Gómez-Randulfe, I., Martínez-Lago, N., Ladra, S., Aparicio, L. A., Bou, G., et al. (2023). Parvimonas micra can translocate from the subgingival sulcus of the human oral cavity to colorectal adenocarcinoma. Molecular Oncology. 18(5). 1143-1173 https://doi.org/10.1002/1878-0261.13506
Abstract
[Abstract] Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients and 30 healthy controls (non-CRC) were collected for microbiome analysis. Saliva (28 non-CRC and 94 CRC), feces (30 non-CRC and 97 CRC), subgingival fluid (20 CRC), and tumor tissue samples (20 CRC) were used for 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis of the abundance, performed with the ANCOM-BC package, adjusting the P-values by the Holm-Bonferroni method, revealed that Parvimonas was significantly over-represented in feces from CRC patients (P-value < 0.001) compared to healthy controls. A total of 11 Parvimonas micra isolates were obtained from the oral cavity and adenocarcinoma of CRC patients. Genome analysis identified a pair of isolates from the same patient that shared 99.2% identity, demonstrating that P. micra can translocate from the subgingival cavity to the gut. The data suggest that P. micra could migrate in a synergistic consortium with other periodontal bacteria. Metatranscriptomics confirmed that oral bacteria were more active in tumor than in non-neoplastic tissues. We suggest that P. micra could be considered as a CRC biomarker detected in non-invasive samples such as feces.
Keywords
Colorectal cancer
Metabarcoding
Metatranscriptomics
Microbiome
Parvimonas micra
Periodontal disease
Metabarcoding
Metatranscriptomics
Microbiome
Parvimonas micra
Periodontal disease
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