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Multifunctional graphene quantum dots: A therapeutic strategy for neurodegenerative diseases by regulating calcium influx, crossing the blood-brain barrier and inhibiting Aβ-protein aggregation
dc.contributor.author | Gómez Pérez, Inmaculada Jénnifer | |
dc.contributor.author | Křížková, Petra | |
dc.contributor.author | Dolečková, Anna | |
dc.contributor.author | Cardo, Lucia | |
dc.contributor.author | Wetzl, Cecilia | |
dc.contributor.author | Pizurova, Nada | |
dc.contributor.author | Prato, Maurizio | |
dc.contributor.author | Medalová, Jiřina | |
dc.contributor.author | Zajickova, Lenka | |
dc.date.accessioned | 2024-08-13T10:10:36Z | |
dc.date.available | 2024-08-13T10:10:36Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Gómez, I. J., Křížková, P., Dolečková, A., Cardo, L., Wetzl, C., Pizúrová, N., Prato, M., Medalová, J., & Zajíčková, L. (2024). Multifunctional graphene quantum dots: A therapeutic strategy for neurodegenerative diseases by regulating calcium influx, crossing the blood-brain barrier and inhibiting Aβ-protein aggregation. Applied Materials Today, 36. https://doi.org/10.1016/J.APMT.2024.102072 | es_ES |
dc.identifier.uri | http://hdl.handle.net/2183/38566 | |
dc.description.abstract | [Abstract] Multifunctional nanoparticles could be the hallmark for the treatment of neurodegenerative diseases. Dissociation of protein aggregates causing neuronal damage and transfer of specific drugs which can downregulate neuronal excitotoxicity by inhibiting glutamatergic N-Methyl-d-Aspartate-receptors (NMDA) and then reducing calcium influx are among the main factors to consider for proper therapy. Here, we present a multiplatform based on nitrogen-doped graphene quantum dots (NGQDs) with such functionalities. The NGQDs were functionalized with Memantine, the clinically used drug, via covalent and non-covalent coupling, and we confirmed that the pharmaceutical activity was not altered. Apart from that, using xCELLigence technology and flow cytometric analysis of ABC transporter function, we uncovered that the ABC transporters of the blood-brain barrier (BBB) do not affect the ability of NGQD to cross BBB. Surprisingly, this study found that NGQDs have an inhibitory effect on NMDA receptors, thus supporting the action of Memantine. Moreover, NGQDs and their derivatives demonstrated the potential to dissociate β-amyloid aggregates while possessing features suitable for bioimaging in various cell lines. | es_ES |
dc.description.sponsorship | This work was supported by Operational Program Research, Development, and Education-Project ‘MSCAfellow4@MUNI’ (No. CZ.02.2.69/0.0/0.0/20_079/0017045). The Spanish Ministry of Universities is acknowledged for a Beatriz Galindo (BG22/000147) and Maria Zambrano funding (RSU.UDC.MZ09 [RSU.UDC.MZ11]) transferred by the European Union-Next GenerationEU. We acknowledge CzechNanoLab Research Infrastructure (LM2018110), supported by the Ministry of Education, Youth and Sports of the Czech Republic (MEYS CR). We are grateful to Prof. Vladimír Šindelář and Prof. Petr Klan for allowing us to use the MW reactor, UV–Vis and fluorescence spectrometer, supported by RECETOX research infrastructure (via MEYS CR under LM2018121). M.P. is AXA Professor and is supported by the European Research Council (ERC-AdG-2019, N. 885323), the Agencia Estatal de Investigación-AEI (“Proyectos I+D+I 2019 -Modalidad Retos Investigación”, N. PID2019–108523RB-I00), by Grant PRE2020–095099. | es_ES |
dc.description.sponsorship | Česká republika. Ministerstvo školství, mládeže a tělovýchovy České republiky; LM2018110 | es_ES |
dc.description.sponsorship | Česká republika. RECETOX; LM2018121 | es_ES |
dc.description.sponsorship | European Union. European Research Council; ERC-AdG-2019 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108523RB-I00/ES/NANODOTS DE CARBONO A MEDIDA COMO NUEVOS MATERIALES MULTIFUNCIONALES SEGUROS PARA APLICACIONES NANO- Y BIO-TECNOLOGICAS | es_ES |
dc.relation.uri | https://doi.org/10.1016/j.apmt.2024.102072 | es_ES |
dc.rights | Attribution 4.0 International CC BY | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Carbon dots | es_ES |
dc.subject | Memantine | es_ES |
dc.subject | Protein aggregation | es_ES |
dc.subject | Blood-brain barrier | es_ES |
dc.subject | Bioimaging | es_ES |
dc.title | Multifunctional graphene quantum dots: A therapeutic strategy for neurodegenerative diseases by regulating calcium influx, crossing the blood-brain barrier and inhibiting Aβ-protein aggregation | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Applied Materials Today | es_ES |
UDC.volume | 36 | es_ES |
UDC.startPage | Article 102072 | es_ES |
dc.identifier.doi | 10.1016/j.apmt.2024.102072 |