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Mass Spectrometry of Carbohydrate-Protein Interactions on a Glycan Array Conjugated to CVD Graphene Surfaces
dc.contributor.author | Merino, Juan P. | |
dc.contributor.author | Serna, Sonia | |
dc.contributor.author | Criado, Alejandro | |
dc.contributor.author | Centeno, Alba | |
dc.contributor.author | Napal, Ilargi | |
dc.contributor.author | Calvo, Javier | |
dc.contributor.author | Zurutuza, Amaia | |
dc.contributor.author | Reichardt, Niels-Christian | |
dc.contributor.author | Prato, Maurizio | |
dc.date.accessioned | 2024-08-08T08:01:00Z | |
dc.date.available | 2024-08-08T08:01:00Z | |
dc.date.issued | 2020-01-06 | |
dc.identifier.citation | Juan Pedro Merino et al 2020 2D Mater. 7 024003 | es_ES |
dc.identifier.issn | 2053-1583 | |
dc.identifier.uri | http://hdl.handle.net/2183/38460 | |
dc.description.abstract | [Abstract]: Mass spectrometry (MS) is a valuable tool for functional genomic, proteomic, and glycomic studies. In particular, the combination of MS with microarrays is a powerful technique for analyzing the activity of carbohydrate processing enzymes and for the identification of car-bohydrate-binding proteins (lectins) in complex matrices. On the other hand, graphene exhi-bits high desorption/ionization efficiency, good conductivity and optical transparency, specifi-cations of a high-performance component for matrix-assisted laser desorption/ionization (MALDI) platforms. Besides, the chemical functionalization of graphene increases the adsor-ption capability of functional biomolecules (e.g. receptors), resulting in very stable interfaces. Taking advantage of the properties of graphene, we developed several modified chemical vapor deposited graphene (CVDG)-based glycan arrays on different substrates including ITO and bare glass, as a potential sensing platform for carbohydrate-lectin interactions, which are involved in a plethora of biological processes. The glycan arrays were fully characterized by MALDI-MS analysis and, in some cases, optical microscopy. | es_ES |
dc.description.sponsorship | M Prato is the recipient of the AXA Chair (2016-2023). A Criado thanks MINECO to his research grant (Juan de la Cierva—Incorporación). This work was supported by the European Union’s Horizon 2020 research and innovation program under Grant Agreements 696656 and 785219 Graphene Flagship. Funding from the Spanish Ministry of Economy, Industry and Competitiveness (project CTQ2016-76721-R to M Prato and Grant CTQ2017-90039-R to N-C Reichardt) is acknowledged. This work was performed under the Maria de Maeztu Units of Excellence Program from the Spanish State Research Agency—Grant No. MDM-2017-0720. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | IOP | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/696656 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/785219 | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de In-novación 2013-2016/IJCI-2016-31113/ES/ | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2016-76721-R/ES | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-90039-R/ES | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MDM-2017-0720/ES/ | es_ES |
dc.relation.uri | https://doi.org/10.1088/2053-1583/ab6030 | es_ES |
dc.rights | © 2020 IOP Publishing Ltd. Original content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence (http://creativecommons.org/licenses/by/3.0). Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. | es_ES |
dc.subject | Graphene | es_ES |
dc.subject | CVD | es_ES |
dc.subject | Chemical modification | es_ES |
dc.subject | LDI | es_ES |
dc.subject | Mass spectrometry | es_ES |
dc.subject | Carbohydrate microarrays | es_ES |
dc.subject | Lectin | es_ES |
dc.title | Mass Spectrometry of Carbohydrate-Protein Interactions on a Glycan Array Conjugated to CVD Graphene Surfaces | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | 2D Materials | es_ES |
UDC.volume | 7 | es_ES |
UDC.issue | 2 | es_ES |
UDC.startPage | Article 024003 | es_ES |
dc.identifier.doi | https://doi.org/10.1088/2053-1583/ab6030 |
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