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Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis
dc.contributor.author | Pinto-Tasende, José A. | |
dc.contributor.author | Fernández-Moreno, Mercedes | |
dc.contributor.author | Vázquez-Mosquera, María E. | |
dc.contributor.author | Fernández-López, Carlos | |
dc.contributor.author | Oreiro, Natividad | |
dc.contributor.author | de-Toro, Javier | |
dc.contributor.author | Blanco García, Francico J | |
dc.date.accessioned | 2024-01-22T10:31:52Z | |
dc.date.available | 2024-01-22T10:31:52Z | |
dc.date.issued | 2023-03-30 | |
dc.identifier.citation | Pinto Tasende JA, Fernandez-Moreno M, Vazquez-Mosquera ME, Fernandez-Lopez JC, Oreiro-Villar N, De Toro Santos FJ, Blanco-García FJ. Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis. BMC Musculoskelet Disord. 2023 Mar 30;24(1):246. | es_ES |
dc.identifier.issn | 1471-2474 | |
dc.identifier.uri | http://hdl.handle.net/2183/35035 | |
dc.description.abstract | [Abstract] Background: Immune and non-immune cells contribute to the pathology of chronic arthritis, and they can contribute to tissue remodeling and repair as well as disease pathogenesis. The present research aimed to analyze inflammation and bone destruction/regeneration biomarkers in patients with psoriatic arthritis (PsA), rheumatoid arthritis (RA), osteoarthritis (OA), and ankylosing spondylitis (AS). Methods: Samples were obtained from the inflamed knee of patients with knee arthritis who had been referred for undergoing arthroscopies. The synovial membrane was processed for pathological description, IHC analysis, and quantification of mRNA expression ratio by qRT-PCR. Serum levels of TGF-β1, IL-23, IL-6, IL-17 A, IL-22, Dkk1, Sclerostin, BMP2, BMP4, Wnt1, and Wnt5a were measured by ELISA. All these data were analyzed and compared with the demographic, clinical, blood tests, and radiological characteristics of the patients. Results: The synovial membrane samples were obtained from 42 patients for IHC, extraction, and purification of RNA for synovial mRNA expression analysis, and serum for measuring protein levels from 38 patients. IHC reactivity for TGF-β1 in the synovial tissue was higher in patients with psoriatic arthritis (p 0.036) and was positively correlated with IL-17 A (r = 0.389, p = 0.012), and Dkk1 (r = 0.388, p = 0.012). Gene expression of the IL-17 A was higher in PsA patients (p = 0.018) and was positively correlated with Dkk1 (r = 0.424, p = 0.022) and negatively correlated with BMP2 (r = -0.396, p = 0.033) and BMP4 (r = -0.472, p = 0.010). It was observed that IHC reactivity for TGF-β1 was higher in patients with erosive PsA (p = 0.024). Conclusions: The IHC reactivity of TGF-β1 in synovial tissue was higher in patients with erosive psoriatic arthritis, and TGF-β1 was in relation to higher levels of gene expression of IL-17 A and Dkk1. | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MICINN/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/ES/PI11%2F00390/Análisis de biomarcadores sinoviales, implicados en la inflamación y destrucción ósea articular en la artritis psoriásica | es_ES |
dc.description.sponsorship | Grant (PI11/00390) from Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica 2008–2011 and co-financed by the ISCIII-Subdirección General de Evaluación y Fomento de la Investigación - Fondo Europeo de Desarrollo Regional (FEDER). | |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.relation.uri | https://doi.org/10.1186/s12891-023-06339-4 | es_ES |
dc.rights | Creative Commons Attribution 4.0 International Licence (CC-BY 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | IHC reactivity | es_ES |
dc.subject | Psoriatic arthritis | es_ES |
dc.subject | Synovial membrane | es_ES |
dc.subject | TGF-β1 | es_ES |
dc.subject | mRNA expression | es_ES |
dc.title | Increased synovial immunohistochemistry reactivity of TGF-β1 in erosive peripheral psoriatic arthritis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | BMC Muskuloskeletal Disorders | es_ES |
UDC.volume | 24 | es_ES |
UDC.startPage | 246 | es_ES |
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