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Bidirectional Modulation of Neuronal Cells Electrical and Mechanical Properties Through Pristine and Functionalized Graphene Substrates
dc.contributor.author | Zummo, Francesca | |
dc.contributor.author | Esposito, Pietro | |
dc.contributor.author | Hou, Huilei | |
dc.contributor.author | Wetzl, Cecilia | |
dc.contributor.author | Rius, Gemma | |
dc.contributor.author | Tkatchenko, Raphaela | |
dc.contributor.author | Guimerà, Anton | |
dc.contributor.author | Godignon, Philippe | |
dc.contributor.author | Prato, Maurizio | |
dc.contributor.author | Prats Alfonso, Elisabet | |
dc.contributor.author | Criado, Alejandro | |
dc.contributor.author | Scaini, Denis | |
dc.date.accessioned | 2022-05-06T13:37:55Z | |
dc.date.available | 2022-05-06T13:37:55Z | |
dc.date.issued | 2022-01-11 | |
dc.identifier.citation | Zummo F, Esposito P, Hou H, Wetzl C, Rius G, Tkatchenko R, Guimera A, Godignon P, Prato M, Prats-Alfonso E, Criado A and Scaini D (2022) Bidirectional Modulation of Neuronal Cells Electrical and Mechanical Properties Through Pristine and Functionalized Graphene Substrates. Front. Neurosci. 15:811348. doi: 10.3389/fnins.2021.811348 | es_ES |
dc.identifier.issn | 1662-453X | |
dc.identifier.uri | http://hdl.handle.net/2183/30619 | |
dc.description.abstract | [Abstract] In recent years, the quest for surface modifications to promote neuronal cell interfacing and modulation has risen. This course is justified by the requirements of emerging technological and medical approaches attempting to effectively interact with central nervous system cells, as in the case of brain-machine interfaces or neuroprosthetic. In that regard, the remarkable cytocompatibility and ease of chemical functionalization characterizing surface-immobilized graphene-based nanomaterials (GBNs) make them increasingly appealing for these purposes. Here, we compared the (morpho)mechanical and functional adaptation of rat primary hippocampal neurons when interfaced with surfaces covered with pristine single-layer graphene (pSLG) and phenylacetic acid-functionalized single-layer graphene (fSLG). Our results confirmed the intrinsic ability of glass-supported single-layer graphene to boost neuronal activity highlighting, conversely, the downturn inducible by the surface insertion of phenylacetic acid moieties. fSLG-interfaced neurons showed a significant reduction in spontaneous postsynaptic currents (PSCs), coupled to reduced cell stiffness and altered focal adhesion organization compared to control samples. Overall, we have here demonstrated that graphene substrates, both pristine and functionalized, could be alternatively used to intrinsically promote or depress neuronal activity in primary hippocampal cultures. | es_ES |
dc.description.sponsorship | This work was funded by the European Union’s Horizon 2020 Research and Innovation Program under the Grant Agreements 785219 and 881603 of the Graphene Flagship. DS acknowledges the support of the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement no. 838902. MP as the recipient of the AXA Bionanotechnology Chair, is grateful to the AXA Research Fund for financial support. This work was performed under the Maria de Maeztu Units of Excellence Program from the Spanish State Research Agency-grant no. MDM-2017- 0720. AC thanks Xunta de Galicia for his research grant Atracción de Talento (no. ED431H 2020/17). GR acknowledges funding from RYC-2016-21412. HH acknowledges funding from Juan de la Cierva – Incorporación no. IJC-2018-037396-I | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED431H 2020/17 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/785219 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/881603 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/838902 | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/MDM-2017-0720/ES/ | |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2016-21412/ES/ | |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/IJC2018-037396-I/ES/ | |
dc.relation.uri | https://doi.org/10.3389/fnins.2021.811348 | es_ES |
dc.rights | Atribución 4.0 Internacional | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Hippocampal neurons | es_ES |
dc.subject | Graphene | es_ES |
dc.subject | Chemical functionalization | es_ES |
dc.subject | Synaptic activity | es_ES |
dc.subject | Cell stiffness | es_ES |
dc.title | Bidirectional Modulation of Neuronal Cells Electrical and Mechanical Properties Through Pristine and Functionalized Graphene Substrates | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Frontiers in Neuroscience | es_ES |
UDC.volume | 15 | es_ES |
UDC.startPage | 811348 | es_ES |
dc.identifier.doi | 10.3389/fnins.2021.811348 |
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