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Selective Detection of Aeromonas Spp. By a Fluorescent Probe Based on the Siderophore Amonabactin
dc.contributor.author | Cisneros Sureda, Javier | |
dc.contributor.author | Rey-Varela, Diego | |
dc.contributor.author | Rodríguez, Jaime | |
dc.contributor.author | Balado, Miguel | |
dc.contributor.author | Lemos, Manuel L. | |
dc.contributor.author | Jiménez, Carlos | |
dc.date.accessioned | 2022-05-06T10:03:56Z | |
dc.date.available | 2022-05-06T10:03:56Z | |
dc.date.issued | 2022-02-03 | |
dc.identifier.citation | Cisneros-Sureda, J., Rey-Varela, D., Rodríguez, J., Balado, M., Lemos, M.L., Jiménez, C., 2022. Selective detection of Aeromonas spp. by a fluorescent probe based on the siderophore amonabactin. Journal of Inorganic Biochemistry 230, 111743. https://doi.org/10.1016/j.jinorgbio.2022.111743 | es_ES |
dc.identifier.issn | 0162-0134 | |
dc.identifier.uri | http://hdl.handle.net/2183/30610 | |
dc.description.abstract | [Abstract] Amonabactins, the siderophores produced by some pathogenic bacteria belonging to Aeromonas genus, can be used for the preparation of conjugates to be imported into the cell using their specific transport machinery. Herein, we report the design and synthesis of a new amonabactin-based fluorescent probe by conjugation of the appropriate amonabactin analogue to sulforhodamine B (AMB-SRB) using a thiol-maleimide click reaction. Growth promotion assays and fluorescence microscopy studies demonstrated that the AMB-SRB fluorescent probe was able to label the fish pathogenic bacterium A. salmonicida subsp. salmonicida through its outer membrane transport (OMT) protein FstC. The labelling of other Aeromonas species, such as the human pathogen A. hydrophila, indicates that this probe can be a very useful molecular tool for studying the amonabactin-dependent iron uptake mechanism. Furthermore, the selective labelling of A. salmonicida and other Aeromonas species in presence of other fish pathogenic bacteria, suggest the potential application of this probe for detection of Aeromonas in water and other fish farming samples through fluorescence assays. | es_ES |
dc.description.sponsorship | This work was supported by grants RTI2018-093634-B-C21/C22 from the State Agency for Research (AEI) of Spain, cofunded by the FEDER Programme from the European Union (MCIN/AEI/10.13039/501100011033/FEDER). M.B. was supported by grant PID2019-103891RJ-100 from MCIN/AEI (Spain). Work in University of Santiago de Compostela and University of A Coruña was also supported by grants GRC2018/018 and GRC2018/039, respectively, from Xunta de Galicia and BLUEBIOLAB (0474_BLUEBIOLAB_1_E), Programme INTERREG V A of Spain-Portugal (POCTEP). D.R-V. thanks Xunta de Galicia (Spain) for a predoctoral fellowship. J.C.-S. thanks to the FPU National Program (FPU16/02060) of the Spanish Ministry of Science, Innovation and Universities for a predoctoral fellowship | es_ES |
dc.description.sponsorship | Xunta de Galicia; GRC2018/018 | es_ES |
dc.description.sponsorship | Xunta de Galicia; GRC2018/039 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093634-B-C21/ES/FACTORES DE VIRULENCIA BACTERIANOS COMO DIANAS TERAPEUTICAS EN PECES: CARACTERIZACION DE SIDEROFOROS Y DESARROLLO DE NUEVOS TRATAMIENTOS CONTRA FORUNCULOSIS Y TENACIBACULOSIS/ | |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093634-B-C22/ES/FACTORES DE VIRULENCIA BACTERIANOS COMO DIANAS TERAPEUTICAS EN PECES: CARACTERIZACION DE SIDEROFOROS Y DESARROLLO DE NUEVOS TRATAMIENTOS CONTRA FORUNCULOSIS Y TENACIBACULOSIS/ | |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-103891RJ-I00/ES/ADAPTACION TRANSCRIPCIONAL DE VIBRIO ANGUILLARUM TRAS LA ADQUISICION DE LA ISLA DE PATOGENICIDAD IRP-HPI: APLICACIONES PARA LA OPTIMIZACION DE VACUNAS FRENTE A LA VIBRIOSIS/ | |
dc.relation | info:eu-repo/grantAgreement/MECD/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FPU16%2F02060/ES/ | |
dc.relation.uri | https://doi.org/10.1016/j.jinorgbio.2022.111743 | es_ES |
dc.rights | Atribución 4.0 Internacional | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Iron | es_ES |
dc.subject | Siderophores | es_ES |
dc.subject | Amonabactin | es_ES |
dc.subject | Aeromonas | es_ES |
dc.subject | Fluorescent probe | es_ES |
dc.subject | Bacteria detection | es_ES |
dc.title | Selective Detection of Aeromonas Spp. By a Fluorescent Probe Based on the Siderophore Amonabactin | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Journal of Inorganic Biochemistry | es_ES |
UDC.volume | 230 | es_ES |
UDC.startPage | 111743 | es_ES |
dc.identifier.doi | 10.1016/j.jinorgbio.2022.111743 |
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