Selective Detection of Aeromonas Spp. By a Fluorescent Probe Based on the Siderophore Amonabactin
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Selective Detection of Aeromonas Spp. By a Fluorescent Probe Based on the Siderophore AmonabactinAutor(es)
Fecha
2022-02-03Cita bibliográfica
Cisneros-Sureda, J., Rey-Varela, D., Rodríguez, J., Balado, M., Lemos, M.L., Jiménez, C., 2022. Selective detection of Aeromonas spp. by a fluorescent probe based on the siderophore amonabactin. Journal of Inorganic Biochemistry 230, 111743. https://doi.org/10.1016/j.jinorgbio.2022.111743
Resumen
[Abstract] Amonabactins, the siderophores produced by some pathogenic bacteria belonging to Aeromonas genus, can be used for the preparation of conjugates to be imported into the cell using their specific transport machinery. Herein, we report the design and synthesis of a new amonabactin-based fluorescent probe by conjugation of the appropriate amonabactin analogue to sulforhodamine B (AMB-SRB) using a thiol-maleimide click reaction. Growth promotion assays and fluorescence microscopy studies demonstrated that the AMB-SRB fluorescent probe was able to label the fish pathogenic bacterium A. salmonicida subsp. salmonicida through its outer membrane transport (OMT) protein FstC. The labelling of other Aeromonas species, such as the human pathogen A. hydrophila, indicates that this probe can be a very useful molecular tool for studying the amonabactin-dependent iron uptake mechanism. Furthermore, the selective labelling of A. salmonicida and other Aeromonas species in presence of other fish pathogenic bacteria, suggest the potential application of this probe for detection of Aeromonas in water and other fish farming samples through fluorescence assays.
Palabras clave
Iron
Siderophores
Amonabactin
Aeromonas
Fluorescent probe
Bacteria detection
Siderophores
Amonabactin
Aeromonas
Fluorescent probe
Bacteria detection
Versión del editor
Derechos
Atribución 4.0 Internacional
ISSN
0162-0134