• Genetic Polymorphism in Cytochrome P450 1B1 in a Spanish Population [Short Communication] 

      Pérez-Cadahía, Beatriz; Valdiglesias, Vanessa; Pásaro, Eduardo; Méndez, Josefina; Laffon, Blanca (Wiley, 2007-06-02)
    • Cytogenetic Effects Induced by Prestige Oil on Human Populations: The Role of Polymorphisms in Genes Involved in Metabolism and DNA Repair 

      Pérez-Cadahía, Beatriz; Laffon, Blanca; Valdiglesias, Vanessa; Pásaro, Eduardo; Méndez, Josefina (Elsevier, 2008-04-18)
      [Abstract] The spill from the oil tanker Prestige (NW Spain, November 2002) was perhaps the biggest ecological disaster that happened worldwide in the last decades. As a consequence of this catastrophe a general concern ...
    • The Organic Selenium Compound Selenomethionine Modulates Bleomycin-Induced DNA Damage and Repair in Human Leukocytes 

      Laffon, Blanca; Valdiglesias, Vanessa; Pásaro, Eduardo; Méndez, Josefina (Springer, 2009-05-08)
      [Abstract] The objective of this work was to evaluate the effects of selenomethionine (SeMet) on the induction, repair, and persistence of DNA damage in human leukocytes challenged with bleomycin (BLM). Comet assay was ...
    • Assessment of Okadaic Acid Effects on Cytotoxicity, DNA Damage and DNA Repair in Human Cells 

      Valdiglesias, Vanessa; Méndez, Josefina; Pásaro, Eduardo; Cemeli, Eduardo; Anderson, Diana; Laffon, Blanca (Elsevier, 2010-05-26)
      [Abstract] Okadaic acid (OA) is a phycotoxin produced by several types of dinoflagellates causing diarrheic shellfish poisoning (DSP) in humans. Symptoms induced by DSP toxins are mainly gastrointestinal, but the intoxication ...
    • Induction of Oxidative DNA Damage by the Marine Toxin Okadaic Acid Depends on Human Cell Type 

      Valdiglesias, Vanessa; Laffon, Blanca; Pásaro, Eduardo; Cemeli, Eduardo; Anderson, Diana; Méndez, Josefina (Elsevier, 2011-03-01)
      [Abstract] The marine toxin okadaic acid (OA) is the main representative of diarrhoeic shellfish poisoning (DSP) toxins. Its ingestion induces nausea, vomiting, diarrhoea and abdominal ache. It has also been found to trigger ...
    • Okadaic Acid Induces Morphological Changes, Apoptosis and Cell Cycle Alterations in Different Human Cell Types 

      Valdiglesias, Vanessa; Laffon, Blanca; Pásaro, Eduardo; Méndez, Josefina (Royal Society of Chemistry (Gran Bretaña), 2011-03-28)
      [Abstract] Okadaic acid (OA) is a marine toxin produced by dinoflagellate species which is frequently accumulated in molluscs usual in the human diet. The exact action mechanism of OA has not been described yet and the ...
    • Evaluation of Okadaic Acid-Induced Genotoxicity in Human Cells Using the Micronucleus Test and γH2AX Analysis 

      Valdiglesias, Vanessa; Laffon, Blanca; Pásaro, Eduardo; Méndez, Josefina (Taylor and Francis, 2011-06-27)
      [Abstract] Marine algal blooms have become a public health concern due to increasing frequency in the environment and severity of exposure consequences. Human intoxications produced by phycotoxins occur globally through ...
    • Identification of Differentially Expressed Genes in SHSY5Y Cells Exposed to Okadaic Acid by Suppression Subtractive Hybridization 

      Valdiglesias, Vanessa; Fernández-Tajes, Juan; Pásaro, Eduardo; Méndez, Josefina; Laffon, Blanca (Springer Nature, 2012-01-27)
      [Abstract] Background: Okadaic acid (OA), a toxin produced by several dinoflagellate species is responsible for frequent food poisonings associated to shellfish consumption. Although several studies have documented the OA ...
    • Inter-Laboratory Variation in DNA Damage Using a Standard Comet Assay Protocol 

      Forchhammer, Lykke; Ersson, Clara; Loft, Steffen; Möller, Lennart; Godschalk, Roger W. L.; Schooten, Frederik J. van; Jones, George D. D.; Higgins, Jennifer A.; Cooke, Marcus; Mistry, Vilas; Karbaschi, Mahsa; Collins, Andrew R.; Azqueta, Amaya; Phillips, David H.; Sozeri, Osman; Routledge, Michael N.; Nelson-Smith, Kirsty; Riso, Patrizia; Porrini, Marisa; Matullo, Giuseppe; Allione, Alessandra; Stępnik, Maciej; Komorowska, Magdalena; Teixeira, João Paulo; Costa, Solange; Corcuera, Laura-Ana; López de Cerain, Adela; Laffon, Blanca; Valdiglesias, Vanessa; Møller, Peter (Oxford University Press, 2012-07-27)
      [Abstract] There are substantial inter-laboratory variations in the levels of DNA damage measured by the comet assay. The aim of this study was to investigate whether adherence to a standard comet assay protocol would ...
    • Neuronal Cytotoxicity and Genotoxicity Induced by Zinc Oxide Nanoparticles 

      Valdiglesias, Vanessa; Costa, C.; Kiliç, Gözde; Costa, Solange; Pásaro, Eduardo; Laffon, Blanca; Teixeira, João Paulo (Elsevier, 2013-03-26)
      [Abstract] Zinc oxide nanoparticles (ZnO NPs) are one of the most abundantly used nanomaterials in consumer products and biomedical applications. As a result, human exposure to these NPs is highly frequent and they have ...
    • Endocrine and Immunological Parameters in Individuals Involved in Prestige Spill Cleanup Tasks Seven Years After the Exposure 

      Laffon, Blanca; Aguilera, Francisco; Ríos-Vázquez, Julia; García Lestón, Julia; Fuchs, Dietmar; Valdiglesias, Vanessa; Pásaro, Eduardo (Elsevier, 2013-06-20)
      [Abstract] In November 2002 the oil tanker Prestige spilled 63,000tonnes of heavy oil off the northwest coast of Spain, impacting more than 1000km of coastline. A general concern led to a huge mobilization of human and ...
    • DNA Damage in Circulating Leukocytes Measured With the Comet Assay May Predict the Risk of Death 

      Bonassi, Stefano; Ceppi, Marcello; Møller, Peter; Azqueta, Amaya; Milić, Mirta; Neri, Monica; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Teixeira, João; Bruzzone, Marco; Da Silva, Juliana; Benedetti, Danieli; Cavallo, Delia; Ursini, Cinzia Lucia; Giovannelli, Lisa; Moretti, Silvia; Riso, Patrizia; Del Bo’, Cristian; Russo, Patrizia; Dobrzyńska, Malgorzata; Goroshinskaya, Irina A.; Surikova, Ekaterina; Staruchova, Marta; Barančoková, Magdaléna; Volkovova, Katarina; Kažimírová, Alena; Smolkova, Bozena; Laffon, Blanca; Valdiglesias, Vanessa; Pastor, Susana; Marcos, Ricard; Hernández, Alba; Gajski, Goran; Spremo-Potparević, Biljana; Živković, Lada; Boutet-Robinet, Elisa; Perdry, Hervé; Lebailly, Pierre; Pérez, Carlos L.; Başaran, Nurşen; Nemeth, Zsuzsanna; Sáfár, Anna; Dusinska, Maria; Collins, Andrew (2021)
      [Abstract] The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, ...
    • The hCOMET Project: International Database Comparison of Results with the Comet Assay in Human Biomonitoring. Baseline Frequency of DNA Damage and Effect of Main Confounders 

      Milić, Mirta; Ceppi, Marcello; Bruzzone, Marco; Azqueta, Amaya; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Møller, Peter; Alija, Avdulla; Anderson, Diana; Andreoli, Cristina; Asllani, Fisnik; Boutet-Robinet, Elisa; Buschini, Annamaria; Cavallo, Delia; Costa, Solange; Da Silva, Juliana; Del Boˊ, Cristian; Djelić, Ninoslav; Dobrzyńska, Malgorzata; Dvořáková, Monika; Gajski, Goran; Galati, Serena; García Lima, Omar; Giovannelli, Lisa; Goroshinskaya, Irina A.; Grindel, Annemarie; Hernández, Alba; Hernández, Carlos; Holven, Kirsten B.; Ibero-Baraibar, Idoia; Ottestad, Inger; Kadioglu, Ela; Kuznetsova, Elena; Ladeira, Carina; Laffon, Blanca; Lamonaca, Palma; Lebailly, Pierre; Louro, Henriqueta; Mandina Cardoso, Tania; Marcon, Francesca; Marcos, Ricard; Moretti, Massimo; Moretti, Silvia; Najafzadeh, Mojgan; Nemeth, Zsuzsanna; Neri, Monica; Novotna, Bozena; Orlow, Irene; Paduchova, Zuzana; Pastor, Susana; Perdry, Hervé; Spremo-Potparević, Biljana; Ramadhani, Dwi; Riso, Patrizia; Rohr, Paula; Rojas, Emilio; Rossner, Pavel; Sardas, Semra; Silva, Maria João; Sirota, Nikolay; Smolkova, Bozena; Staruchova, Marta; Stetina, Rudolf; Stopper, Helga; Ursini, Cinzia Lucia; Valdiglesias, Vanessa; Valverde, Mahara; Volkovova, Katarina; Wagner, Karl-Heinz; Živković, Lada; Dušinská, Maria; Collins, Andrew R.; Bonassi, Stefano; Teixeira, João; Andrade, Vanessa Moraes de; Bankoglu, Ezgi Eylül; Barančoková, Magdaléna; Pereira, Cristiana; Dimitrijević-Srećković, Vesna; Sáfár, Anna; Surikova, Ekaterina; Vodička, Pavel; Başaran, Nurşen; Costa, C.; Ďuračková, Zdeňka; Kažimírová, Alena; Gützkow, Kristine Bjerve; Ulven, Stine Marie (2021)
      [Abstract] The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification ...
    • Suitability of the In Vitro Cytokinesis-Block Micronucleus Test for Genotoxicity Assessment of TiO2 Nanoparticles on SH-SY5Y Cells 

      Fernández-Bertólez, Natalia; Brandão, Fátima; Costa, C.; Pásaro, Eduardo; Teixeira, João; Laffon, Blanca; Valdiglesias, Vanessa (2021)
      [Abstract] Standard toxicity tests might not be fully adequate for evaluating nanomaterials since their unique features are also responsible for unexpected interactions. The in vitro cytokinesis-block micronucleus (CBMN) ...
    • Exploring Early Detection of Frailty Syndrome in Older Adults: Evaluation of Oxi-Immune Markers, Clinical Parameters and Modifiable Risk Factors 

      Teixeira Gomes, Armanda; Laffon, Blanca; Valdiglesias, Vanessa; Gostner, Johanna; Felder, Thomas; Costa, C.; Madureira, Joana; Fuchs, Dietmar; Teixeira, João; Costa, Solange (2021)
      [Abstract] Ageing is accompanied with a decline in several physiological systems. Frailty is an age-related syndrome correlated to the loss of homeostasis and increased vulnerability to stressors, which is associated with ...
    • Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage 

      Valdiglesias, Vanessa; Fernández-Bertólez, Natalia; Lema-Arranz, Carlota; Rodríguez-Fernández, Raquel; Pásaro, Eduardo; Reis, Ana Teresa; Teixeira, João; Costa, C.; Laffon, Blanca (MDPI, 2021-07-27)
      [Abstract] Metal oxide nanoparticles (NPs) have a wide variety of applications in many consumer products and biomedical practices. As a result, human exposure to these nanomaterials is highly frequent, becoming an issue ...
    • Design, Synthesis, Evaluation and Structure of Allenic 1α,25-Dihydroxyvitamin D₃ Analogs with Locked Mobility at C-17 

      Fraga, Ramón; Len, Kateryna; Lutzing, Regis; Laverny, Gilles; Loureiro, Julián; Maestro, Miguel; Rochel, Natacha; Rodríguez-Borges, Enrique; Mouriño, Antonio (Wiley, 2021-08-11)
      [Abstract] Vitamin D receptor ligands have potential for the treatment of hyperproliferative diseases and disorders related to the immune system. However, hypercalcemic effects limit their therapeutical uses and call for ...
    • Suitability of Salivary Leucocytes to Assess DNA Repair Ability in Human Biomonitoring Studies by the Challenge-Comet Assay 

      Fernández-Bertólez, Natalia; Lema Arranz, Carlota; Fraga, S.; Teixeira, Joao; Pasaro, Eduardo; Lorenzo-López, Laura; Valdiglesias, Vanessa; Laffon, Blanca (Elsevier, 2022)
      [Abstract] The challenge-comet assay is a simple but effective approach that provides a quantitative and functional determination of DNA repair ability, and allows to monitor the kinetics of repair process. Peripheral blood ...
    • Cytotoxicity and Genotoxicity of Nanomaterials [Editorial] 

      Valdiglesias, Vanessa (MDPI, 2022-02-14)
    • The Centennial Collection of VDR Ligands: Metabolites, Analogs, Hybrids and Non-Secosteroidal Ligands [Review] 

      Maestro, Miguel; Seoane, Samuel (MDPI, 2022-11-21)
      [Abstract] Since the discovery of vitamin D a century ago, a great number of metabolites, analogs, hybrids and nonsteroidal VDR ligands have been developed. An enormous effort has been made to synthesize compounds which ...