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Identification of a Distinct Lipidomic Profile in the Osteoarthritic Synovial Membrane by Mass Spectrometry Imaging
dc.contributor.author | Rocha Loureda, Beatriz | |
dc.contributor.author | Cillero-Pastor, B. | |
dc.contributor.author | Ruiz-Romero, Cristina | |
dc.contributor.author | Paine, Martin R.L. | |
dc.contributor.author | Cañete, Juan D. | |
dc.contributor.author | Heeren, Ron M.A. | |
dc.contributor.author | Blanco García, Francisco J | |
dc.date.accessioned | 2021-09-22T09:15:57Z | |
dc.date.issued | 2021-05 | |
dc.identifier.citation | Rocha B, Cillero-Pastor B, Ruiz-Romero C, Paine MRL, Cañete JD, Heeren RMA, et al. Identification of a distinct lipidomic profile in the osteoarthritic synovial membrane by mass spectrometry imaging. Osteoarthritis Cartilage.2021;29(5):750-761. Doi: 10.1016/j.joca.2020.12.025 | es_ES |
dc.identifier.issn | 1063-4584 | |
dc.identifier.uri | http://hdl.handle.net/2183/28505 | |
dc.description.abstract | [Abstract] Objective: Synovial inflammation is one of the most characteristic events in different types of arthritis, including Osteoarthritis (OA). Emerging evidence also suggests the involvement of lipids in the regulation of inflammatory processes. The aim of this study was to elucidate the heterogeneity and spatial distribution of lipids in the OA synovial membrane and explore their putative involvement in inflammation. Method: The abundance and distribution of lipids were examined in human synovial membranes. To this end, histological cuts from this tissue were analysed by matrix-assisted laser desorption ionization - mass spectrometry imaging (MALDI-MSI). The lipidomic profile of OA synovium was characterized and compared with healthy and other forms of inflammatory arthropathies as Rheumatoid Arthritis (RA) and Psoriatic Arthritis (PsA) using principal component analysis and discriminant analysis methods. Lipid identification was undertaken by tandem MS analyses and database queries. Results: Our results reveal differential and characteristic lipidomic profiles between OA and control samples. Specifically, we unveiled that OA synovium presents elevated levels of phosphatidylcholines, fatty acids and lysophosphatidic acids and lower levels of lysophosphatidylcholines compared to control tissues. The spatial distribution of particular glycerophospholipids was also correlated with hypertrophic, inflamed or vascularized synovial areas. Compared with other inflammatory arthritis, the OA tissue showed lower amounts of phosphatidylethanolamine-based plasmalogens. Conclusions: This study provides a novel insight into the lipid profiles of synovial membrane and differences in abundance between OA and control tissues. The lipidomic alterations improves understanding of the pathogenic mechanisms of OA and may be important for its diagnosis. | es_ES |
dc.description.sponsorship | This work is supported by grants from Fondo Investigación Sanitaria-Spain (PI16/02124, PI17/00404 PI19/01206 and RETIC-RIER-RD16/0012/0002), integrated in the National Plan for Scientific Program, Development and Technological Innovation 2013–2016 and funded by the ISCIII-General Subdirection of Assessment and Promotion of Research – European Regional Development Fund (FEDER) “A way of making Europe”. This study is also supported by AE CICA-INIBIC (ED431E 2018/03) and grants IN607A2017/11 and IN607D2020/10 from Xunta de Galicia. The Biomedical Research Networking Center (CIBER) is an initiative from Instituto de Salud Carlos III (ISCIII). The Proteomics Unit of GIR belongs to ProteoRed, PRB3- ISCIII (PT17/0019/0014). B.R. has been supported by Xunta de Galicia (IN606B-2016/004). The research was partially performed within the M4I research program, financially supported by the Dutch Province of Limburg as part of the “LINK” program | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED431E 2018/03 | es_ES |
dc.description.sponsorship | Xunta de Galicia; IN607A2017/11 | es_ES |
dc.description.sponsorship | Xunta de Galicia; IN607D2020/10 | es_ES |
dc.description.sponsorship | Xunta de Galicia; IN606B-2016/004 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI16%2F02124/ES/Determinación de índices predictivos de diagnóstico y pronóstico de artrosis de rodilla mediante la validación de biomarcadores proteicos | |
dc.relation | info:eu-repo/grantAgreement/MICINN/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PI17%2F00404/ES/Identificación y validación de biomarcadores circulantes predictivos de respuesta a estrategias de terapia biológica en pacientes con artritis reumatoide (BIO-RESTER) | |
dc.relation | info:eu-repo/grantAgreement/MICINN/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PI19%2F01206/ES/VALIDACIÓN CLÍNICA DE NUEVOS BIOMARCADORES PREDICTIVOS DE DIAGNÓSTICO Y PRONÓSTICO EN ARTROSIS: EL PROYECTO HPP | |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RD16%2F0012%2F0002/ES/Red de Investigación en Inflamación y Enfermedades Reumáticas (RIER) | |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PT17%2F0019%2F0014/ES/PLATAFORMA DE PROTEOMICA, GENOTIPADO Y LÍNEAS CELULARES | |
dc.relation.uri | https://doi.org/10.1016/j.joca.2020.12.025 | es_ES |
dc.rights | Atribución-NoComercial-SinDerivadas 4.0 Internacional | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Glycerophospholipids | es_ES |
dc.subject | Mass spectrometry imaging | es_ES |
dc.subject | Osteoarthritis | es_ES |
dc.subject | Synovial membrane | es_ES |
dc.title | Identification of a Distinct Lipidomic Profile in the Osteoarthritic Synovial Membrane by Mass Spectrometry Imaging | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.date.embargoEndDate | 2022-05-01 | es_ES |
dc.date.embargoLift | 2022-05-01 | |
UDC.journalTitle | Ostearthritis and Cartilage | es_ES |
UDC.volume | 29 | es_ES |
UDC.issue | 5 | es_ES |
UDC.startPage | 750 | es_ES |
UDC.endPage | 761 | es_ES |
dc.identifier.doi | 10.1016/j.joca.2020.12.025 |
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