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Circulating miR-200c as a diagnostic and prognostic biomarker for gastric cancer
dc.contributor.author | Valladares-Ayerbes, Manuel | |
dc.contributor.author | Reboredo, Margarita | |
dc.contributor.author | Medina Villaamil, Vanessa | |
dc.contributor.author | Iglesias-Díaz, Pilar | |
dc.contributor.author | Lorenzo-Patiño, María J. | |
dc.contributor.author | Haz, Mar | |
dc.contributor.author | Santamarina, Isabel | |
dc.contributor.author | Blanco, Moisés | |
dc.contributor.author | Fernández-Tajes, Juan | |
dc.contributor.author | Quindós-Varela, María | |
dc.contributor.author | Carral, Alberto | |
dc.contributor.author | Figueroa, Angélica | |
dc.contributor.author | Antón-Aparicio, Luis M. | |
dc.contributor.author | Calvo, Lourdes | |
dc.date.accessioned | 2017-08-29T10:13:38Z | |
dc.date.available | 2017-08-29T10:13:38Z | |
dc.date.issued | 2012-09-06 | |
dc.identifier.citation | Valladares-Ayerbes M, Reboredo M, Medina-Villaamil V, et al. Circulating miR-200c as a diagnostic and prognostic biomarker for gastric cancer. J Transl Med [Internet]. 2012 Sep 6 [acceso 2017 Ago 29];10:186. Disponible en: https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-10-186 | es_ES |
dc.identifier.issn | 1479-5876 | |
dc.identifier.uri | http://hdl.handle.net/2183/19366 | |
dc.description.abstract | [Abstract] Background. MicroRNAs are aberrantly expressed and correlate with tumourigenesis and the progression of solid tumours. The miR-200 family determines the epithelial phenotype of cancer cells and regulates invasiveness and migration. Thus, we hypothesised that the quantitative detection of the miR-200 family as epithelial-specific microRNAs in the blood could be a useful clinical biomarker for gastric cancer (GC). Methods. We initially validated the expression levels of miR-200a, 200b, 200c and 141 in GC cell lines (n = 2) and blood from healthy controls (n = 19) using real-time quantitative reverse transcription PCR (qRT-PCR). The microarray expression profiles of the miR-200 family in 160 paired samples of non-tumour gastric mucosae and GC were downloaded through ArrayExpress and analysed. MiR-200c was selected for clinical validation. The qRT-PCR prospective assessment of miR-200c was performed using 67 blood samples (52 stage I-IV GC patients and 15 controls); the area under the receiver operating characteristic curve (AUC-ROC) was estimated. The Kaplan-Meier and Breslow-Wilcoxon tests were used to assess the correlation of miR-200c with overall and progression-free survival (OS and PFS). Multivariate analyses were performed using the Cox model. Results. The miR-200c blood expression levels in GC patients were significantly higher than in normal controls (p = 0.018). The AUC-ROC was 0.715 (p = 0.012). The sensitivity, specificity and accuracy rates of 65.4%, 100% and 73.1%, respectively, were observed. The levels of miR-200c in the blood above the cutoff defined by the ROC curve was found in 17.6% of stage I-II GC patients, 20.6% of stage III patients and 67.7% of stage IV patients (p < 0.001). The miR-200c expression levels were not associated with clinical or pathological characteristics or recent surgical procedures. There was a correlation (p = 0.016) with the number of lymph node metastases and the increased expression levels of miR-200c in blood were significantly associated with a poor OS (median OS, 9 vs 24 months; p = 0.016) and PFS (median PFS, 4 vs 11 months; p = 0.044). Multivariate analyses confirmed that the upregulation of miR-200c in the blood was associated with OS (HR = 2.24; p = 0.028) and PFS (HR = 2.27; p = 0.028), independent of clinical covariates. Conclusions. These data suggest that increased miR-200c levels are detected in the blood of gastric cancer patients. MiR-200c has the potential to be a predictor of progression and survival. | es_ES |
dc.description.sponsorship | Instituto de Salud Carlos III; PI061541 | es_ES |
dc.description.sponsorship | Xunta de Galicia; PS08/77 | es_ES |
dc.language.iso | spa | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.relation.uri | http://dx.doi.org/10.1186/1479-5876-10-186 | es_ES |
dc.rights | Atribución 3.0 España | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Gastric cancer | es_ES |
dc.subject | MicroRNA | es_ES |
dc.subject | mir-200 | es_ES |
dc.subject | Blood | es_ES |
dc.subject | Biomarker | es_ES |
dc.subject | Prognostic factors | es_ES |
dc.title | Circulating miR-200c as a diagnostic and prognostic biomarker for gastric cancer | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Journal of Translational Medicine | es_ES |
UDC.volume | 10 | es_ES |
UDC.startPage | 186 | es_ES |
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