• Biochemical and structural characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family 

      Miguel-Ruano, Vega; Rivera, Ivanna; Rajkovic, Jelena; Kamila, Knapik; Torrado Agrasar, Ana; Otero, J. M.; Beneventi, Elisa; Becerra, Manuel; Sánchez-Costa, Mercedes; Hidalgo, Aurelio; Berenguer, José; González-Siso, María-Isabel; Cruces, Jacobo; Rúa Rodríguez, María Luisa; Hermoso, Juan (Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology, 2021)
      [Abstract]: A novel esterase, EstD11, has been discovered in a hot spring metagenomic library. It is a thermophilic and thermostable esterase with an optimum temperature of 60 C. A detailed substrate preference analysis ...
    • Reactivity of a Recombinant Esterase from Thermus thermophilus HB27 in Aqueous and Organic Media 

      González González, Roberto; Fuciños, Pablo; Beneventi, Elisa; López-López, Olalla; Pampín, Begoña; Rodríguez, Ramón; González-Siso, María-Isabel; Cruces, Jacobo; Rúa Rodríguez, María Luisa (MDPI, 2022-04-27)
      [Abstract] The thermoalkalophilic membrane-associated esterase E34Tt from Thermus thermophilus HB27 was cloned and expressed in Kluyveromyces lactis (KLEST-3S esterase). The recombinant enzyme was tested as a biocatalyst ...