Browsing by Author "González-Siso, María-Isabel"
Now showing items 1-20 of 28
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AMWEst, a New Thermostable and Detergent-Tolerant Esterase Retrieved from the Albian Aquifer
Adjeroud, Moussa; Kecha, Mouloud; Escuder-Rodríguez, Juan-José; Becerra, Manuel; González-Siso, María-Isabel (Springer Nature, 2024-01-10)[Abstract] A fosmid library was constructed with the metagenomic DNA from the high-temperature sediment-rich water of the Albian aquifer (Algeria). Functional screening of this library was subsequently done looking for ... -
Analítica de aprendizaje de una materia optativa del grado de Biología
Becerra, Manuel; González-Siso, María-Isabel (Universidade da Coruña, Servizo de Publicacións, 2021)[Resumen] El uso de plataformas virtuales de docencia por los estudiantes genera una gran cantidad de huellas y el reto consiste en analizar estos datos para sacar conclusiones del proceso de enseñanza-aprendizaje. En el ... -
Biochemical and structural characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family
Miguel-Ruano, Vega; Rivera, Ivanna; Rajkovic, Jelena; Kamila, Knapik; Torrado Agrasar, Ana; Otero, J. M.; Beneventi, Elisa; Becerra, Manuel; Sánchez-Costa, Mercedes; Hidalgo, Aurelio; Berenguer, José; González-Siso, María-Isabel; Cruces, Jacobo; Rúa Rodríguez, María Luisa; Hermoso, Juan (Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology, 2021)[Abstract]: A novel esterase, EstD11, has been discovered in a hot spring metagenomic library. It is a thermophilic and thermostable esterase with an optimum temperature of 60 C. A detailed substrate preference analysis ... -
Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol
Álvarez-Cao, María-Efigenia; Cerdán, María Esperanza; González-Siso, María-Isabel; Becerra, Manuel (Frontiers, 2019-03-07)[Abstract] Molasses are sub-products of the sugar industry, rich in sucrose and containing other sugars like raffinose, glucose, and fructose. Alpha-galactosidases (EC. 3.2.1.22) catalyze the hydrolysis of alpha-(1,6) bonds ... -
Bioprospecting for Thermozymes and Characterization of a Novel Lipolytic Thermozyme Belonging to the SGNH/GDSL Family of Hydrolases
Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Saavedra-Bouza, Almudena; González-Siso, María-Isabel; Becerra, Manuel (MDPI, 2022-05-20)[Abstract] Functional screenings were conducted on two metagenomic libraries from hot springs in order to find novel thermozymes with potential biotechnological applications. These included enzymes acting on plant cell ... -
Cellulases from thermophiles found by metagenomics
Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Cerdán, María Esperanza; Rodríguez-Belmonte, Esther; Becerra, Manuel; González-Siso, María-Isabel (MDPI, 2018-07-10)[Abstract] Cellulases are a heterogeneous group of enzymes that synergistically catalyze the hydrolysis of cellulose, the major component of plant biomass. Such reaction has biotechnological applications in a broad spectrum ... -
Characterization of a Novel Thermophilic Metagenomic GH5 Endoglucanase Heterologously Expressed in Escherichia coli and Saccharomyces cerevisiae
Escuder-Rodríguez, Juan-José; González-Suárez, María; De Castro de Antonio, María Eugenia; Saavedra-Bouza, Almudena; Becerra, Manuel; González-Siso, María-Isabel (BMC, 2022-07-07)[Abstract] Background: Endoglucanases from thermophilic microorganisms are a valuable resource as they can be used in a wide variety of biotechnological applications including the valorisation of biomass and the production ... -
Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide
De Castro de Antonio, María Eugenia; Escuder-Rodríguez, Juan-José; Becerra, Manuel; Rodríguez-Belmonte, Esther; González-Siso, María-Isabel (Frontiers, 2021-11-26)[Abstract] With their circumneutral pH and their moderate temperature (66 and 68°C, respectively), As Burgas and Muiño da Veiga are two important human-use hot springs, previously studied with traditional culture methods, ... -
Diseño de una materia de máster para la docencia a distancia
Becerra, Manuel; González-Siso, María-Isabel (Universidade da Coruña. Servizo de publicacións., 2018)[Resumen] Se han elaborado una serie de contenidos para adaptar una materia de máster a la modalidad de docencia a distancia utilizando como plataforma de teleenseñanza Moodle. La materia se estructuró en unidades ... -
Editorial for the special issue: Thermophiles and Thermozymes
González-Siso, María-Isabel (MDPI, 2019-02-27)[Abstract:] Heat-loving microorganisms or thermophiles arouse noticeable scientific interest nowadays, not only with the aim to elucidate the mystery of life at high temperatures, but also due to the huge field of ... -
Exploring the Taxonomical and Functional Profile of As Burgas Hot Spring Focusing On Thermostable β-galactosidases
De Castro de Antonio, María Eugenia; Doane, Michael P.; Dinsdale, Elizabeth Ann; Rodríguez-Belmonte, Esther; González-Siso, María-Isabel (Springer Nature, 2021-01-08)[Abstract] In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate ... -
Genomics of Extreme Environments: Unveiling the Secrets of Survival [Editorial]
Goh, Kian Mau; González-Siso, María-Isabel; Sani, Rajesh K. (Springer Nature, 2023-12-05)[Abstract] Life on Earth has displayed remarkable adaptability to the harshest environments, spanning polar regions, scorching deserts, abyssal oceans, lightless caves, noxious lakes, boiling hot springs, and nuclear waste ... -
Hot Spring Metagenomics
López-López, Olalla; Cerdán, María Esperanza; González-Siso, María-Isabel (Multidisciplinary Digital Publishing Institute, 2013)Hot springs have been investigated since the XIX century, but isolation and examination of their thermophilic microbial inhabitants did not start until the 1950s. Many thermophilic microorganisms and their viruses have ... -
Insights on Microbial Communities Inhabiting Non-Volcanic Hot Springs
Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Saavedra-Bouza, Almudena; Becerra, Manuel; González-Siso, María-Isabel (MDPI, 2022-10-13)[Abstract] The northwest of Spain has an abundance of non-volcanic hot springs that, until recently, had only been used for thermalism activities. One of such hot springs, Muiño da Veiga, has now been explored using ... -
Ixr1 Regulates Ribosomal Gene Transcription and Yeast Response to Cisplatin
Vizoso-Vázquez, Ángel; Lamas, Mónica; González-Siso, María-Isabel; Cerdán, María Esperanza (Springer Nature, 2018-02-15)[Abstract] Ixr1 is a Saccharomyces cerevisiae HMGB protein that regulates the hypoxic regulon and also controls the expression of other genes involved in the oxidative stress response or re-adaptation of catabolic and ... -
Métodos experimentales para el estudio de los genomas
González-Siso, María-Isabel; Rodríguez Torres, Ana María (Universidade da Coruña, 1997) -
Microbial diversity analysis and screening for novel xylanase enzymes from the sediment of the Lobios Hot Spring in Spain
Knapik, Kamila; Becerra, Manuel; González-Siso, María-Isabel (Springer Nature, 2019-08-01)[Abstract] Here, we describe the metagenome composition of a microbial community in a hot spring sediment as well as a sequence-based and function-based screening of the metagenome for identification of novel xylanases. ... -
Novel Microbial Enzymes with Industrial Applications [Editorial]
González-Siso, María-Isabel; Becerra, Manuel (MDPI, 2023-04-10) -
Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides
Álvarez Cao, María E.; Cerdán, María Esperanza; González-Siso, María-Isabel; Becerra, Manuel (BMC, 2019-10-10)[Abstract] Background: α-Galactosidases are enzymes that act on galactosides present in many vegetables, mainly legumes and cereals, have growing importance with respect to our diet. For this reason, the use of their ... -
Production and characterization of an extracellular acid protease from thermophilic Brevibacillus sp. OA30 isolated from an Algerian hot spring
Gomri, Mohamed Amine; Rico Díaz, Agustín; Escuder-Rodríguez, Juan-José; El Moulouk Khaldi, Tedj; González-Siso, María-Isabel; Kharroub, Karima (MDPI, 2018-04-12)[Abstract] Proteases have numerous biotechnological applications and the bioprospection for newly-thermostable proteases from the great biodiversity of thermophilic microorganisms inhabiting hot environments, such as ...
