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Conditioned Medium from Human Uterine Cervical Stem Cells Regulates Oxidative Stress and Angiogenesis of Retinal Pigment Epithelial Cells
dc.contributor.author | Bermúdez, María José | |
dc.contributor.author | Eiro, Noemi | |
dc.contributor.author | Sendón Lago, Juan José | |
dc.contributor.author | Cid, Sandra | |
dc.contributor.author | Saa, Jorge | |
dc.contributor.author | Pablo, Nagore de | |
dc.contributor.author | Vega, Belen | |
dc.contributor.author | Perez-Fernandez, Roman | |
dc.contributor.author | Vizoso, Francisco | |
dc.date.accessioned | 2024-08-29T10:30:51Z | |
dc.date.available | 2024-08-29T10:30:51Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Eiro, N., Sendon-Lago, J., Cid, S., Saa, J., de Pablo, N., Vega, B., Bermudez, M. A., Perez-Fernandez, R., & Vizoso, F. J. (2022). Conditioned Medium from Human Uterine Cervical Stem Cells Regulates Oxidative Stress and Angiogenesis of Retinal Pigment Epithelial Cells. Ophthalmic research, 65(5), 556-565. https://doi.org/10.1159/000524484 | es_ES |
dc.identifier.uri | http://hdl.handle.net/2183/38756 | |
dc.description.abstract | [Abstract] Introduction: Retinal homeostasis is essential to avoid retinal pigment epithelium (RPE) damage resulting in photoreceptor death and blindness. Mesenchymal stem cells-based cell therapy could contribute to the maintenance of the retinal homeostasis. We have explored the effect of human uterine cervical stem cells (hUCESCs)-conditioned medium (hUCESC-CM) on RPE cells under oxidative stress condition. Methods: ARPE-19 cells were treated with hydrogen peroxide (H2O2) in the presence or absence of hUCESC-CM. qRT-PCR and Western blot were used to evaluate the expression of oxidative stress-related (HO-1, GCLC, and HSPB1) and vasculogenesis-related (VEGFA, PDGFA, and PDGFB) factors. Also, we assessed in vitro effects of hUCESC-CM on endothelial-cell (HUVEC) tube formation. Results: mRNA expression of HO-1, GCLC, HSPB1, VEGFA, PDGFA, and PDGFB were significantly increased in ARPE-19 cells treated with H2O2 + hUCESC-CM compared to cells treated with H2O2 only. Regarding the tube formation assay, HUVEC treated with supernatant from ARPE-19 cells treated with H2O2 + hUCESC-CM showed a significant increase in average vessel length, number of capillary-like junctions, and average of vessels area compared with HUVEC treated with supernatant from ARPE-19 cells treated with H2O2 only. Conclusion: Our results show potential therapeutic effects of hUCESC-CM on RPE, such as protection from damage by oxidative stress, stimulation of detoxifying genes, and a better vascularization. | es_ES |
dc.description.sponsorship | This research was supported by grants to R.P.-F. from Ministerio de Economia y Competividad (SAF2015-69221-R), to N.E. and F.J.V from Instituto de Salud Carlos III (PI20/01122) and cofunded by European Union (ERDF/ESF, “Investing in your future”), by Fundación para la Investigación con Células Madre Uterinas (FICEMU) to R.P.F. and F.J.V., and by ONCE (Organización Nacional de Ciegos Españoles) to F.J.V. S.C. was recipient of a fellowship financed by the Gobierno del Principado de Asturias “Severo Ochoa” PhD Program under Grant BP14-128. The APC was funded by Instituto de Salud Carlos III (PI20/01122). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | NLM (Medline) | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 /SAF2015-69221-R/ES/PIT-1, VITAMINA D Y ESTROMA EN CANCER DE MAMA | es_ES |
dc.relation | info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PI20%2F01122/ES/CARACTERIZACION Y EVALUACION FUNCIONAL DE EXOSOMAS DE CELULAS MADRE MESENQUIMALES DEL CERVIX UTERINO HUMANO COMO NUEVA ESTRATEGIA TERAPEUTICA REGENERATIVA Y ANTI-INFLAMATORIA | es_ES |
dc.relation.uri | https://doi.org/10.1159/000524484 | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-4.0 International License (CC BY-NC) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/es/ | * |
dc.subject | Mesenchymal stem cells | es_ES |
dc.subject | Human uterine cervical stem cells | es_ES |
dc.subject | Conditioned medium | es_ES |
dc.subject | Secretome | es_ES |
dc.subject | Retinal pigment epithelium | es_ES |
dc.subject | Oxidative stress | es_ES |
dc.subject | Angiogenesis | es_ES |
dc.title | Conditioned Medium from Human Uterine Cervical Stem Cells Regulates Oxidative Stress and Angiogenesis of Retinal Pigment Epithelial Cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Ophthalmic research | es_ES |
UDC.volume | 65 | es_ES |
UDC.issue | 5 | es_ES |
UDC.startPage | 556 | es_ES |
UDC.endPage | 565 | es_ES |
dc.identifier.doi | 10.1159/000524484 |
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