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Gene-Activated Hyaluronic Acid-Based Cryogels for Cartilage Tissue Engineering
dc.contributor.author | López-Seijas, Junquera | |
dc.contributor.author | Rey-Rico, Ana | |
dc.contributor.author | Carballo-Pedrares, Natalia | |
dc.contributor.author | Miranda-Balbuena, Diego | |
dc.contributor.author | Lamas Criado, Ibán | |
dc.contributor.author | Yáñez, Julián | |
dc.date.accessioned | 2024-08-06T09:32:13Z | |
dc.date.available | 2024-08-06T09:32:13Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Carballo-Pedrares, N., López-Seijas, J., Miranda-Balbuena, D., Lamas, I., Yáñez, J., & Rey-Rico, A. (2023). Gene-activated hyaluronic acid-based cryogels for cartilage tissue engineering. Journal of Controlled Release, 362, 606-619. https://doi.org/10.1016/J.JCONREL.2023.09.008 | es_ES |
dc.identifier.uri | http://hdl.handle.net/2183/38417 | |
dc.description | Financiado para publicación en acceso aberto: Universidade da Coruña/CISUG | es_ES |
dc.description.abstract | [Abstract] Articular cartilage injuries are very frequent lesions that if left untreated may degenerate into osteoarthritis. Gene transfer to mesenchymal stem cells (MSCs) provides a powerful approach to treat these lesions by promoting their chondrogenic differentiation into the appropriate cartilage phenotype. Non-viral vectors constitute the safest gene transfer tools, as they avoid important concerns of viral systems including immunogenicity and insertional mutagenesis. However, non-viral gene transfer usually led to lower transfection efficiencies when compared with their viral counterparts. Biomaterial-guided gene delivery has emerged as a promising alternative to increase non-viral gene transfer efficiency by achieving sustained delivery of the candidate gene into cellular microenvironment. In the present study, we designed hyaluronic acid-based gene-activated cryogels (HACGs) encapsulating a novel formulation of non-viral vectors based on niosomes (P80PX) to promote MSCs in situ transfection. The developed HACG P80PX systems showed suitable physicochemical properties to promote MSCs in situ transfection with very low cytotoxicity. Incorporation of a plasmid encoding for the transcription factor SOX9 (psox9) into HACG P80PX systems led to an effective MSCs chondrogenic differentiation with reduced expression of fibrocartilage and hypertrophic markers. The capacity of the developed systems to restore cartilage extracellular matrix was further confirmed in an ex vivo model of chondral defect. | es_ES |
dc.description.sponsorship | Funding: The work was supported by MICINN [RTI2018-099389-A-100, RYC2018-025617-I], Xunta de Galicia [ED431F2021/10] and Deputación da Coruña [BINV-CS/2022, 2022000021445]. Acknowledgements: We would like to acknowledge Prof. M. Cucchiarini for providing pACP-hsox9 (psox9, bp 6,915). We also thank Biobanco de A Coruña from SERGAS for providing biological samples, BASF (Ludwigshafen, Germany) for supplying the poloxamer 407 and Universidade da Coruña/CISUG for the funding for open access charge. | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED431F2021/10 | es_ES |
dc.description.sponsorship | Deputación da Coruña; BINV-CS/2022, 2022000021445 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099389-A-I00/ES/CRIOGELES ACTIVADOS POR GENES PARA REPARACION DE CARTILAGO | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC2018-025617-I/ES/Targeted and controlled delivery systems of bioactive molecules | es_ES |
dc.relation.uri | https://doi.org/10.1016/j.jconrel.2023.09.008 | es_ES |
dc.rights | Creative Commons Attribution-Noncommercial-Noderivatives 4.0 International CC-BY-NC-ND | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cartilage repair | es_ES |
dc.subject | Gene therapy | es_ES |
dc.subject | Non-viral vectors | es_ES |
dc.subject | Niosomes | es_ES |
dc.subject | P80PX | es_ES |
dc.subject | Gene activated cryogel | es_ES |
dc.subject | HACG | es_ES |
dc.subject | Mesenchymal stem cells | es_ES |
dc.title | Gene-Activated Hyaluronic Acid-Based Cryogels for Cartilage Tissue Engineering | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Journal of Controlled Release | es_ES |
UDC.volume | 362 | es_ES |
UDC.startPage | 606 | es_ES |
UDC.endPage | 619 | es_ES |
dc.identifier.doi | 10.1016/j.jconrel.2023.09.008 |