Mostrar o rexistro simple do ítem
Hydrogel-Guided, rAAV-Mediated IGF-I Overexpression Enables Long-Term Cartilage Repair and Protection against Perifocal Osteoarthritis in a Large-Animal Full-Thickness Chondral Defect Model at One Year In Vivo
dc.contributor.author | Rey-Rico, Ana | |
dc.contributor.author | Madry, Henning | |
dc.contributor.author | Venkatesan, Jagadeesh Kumar | |
dc.contributor.author | Schmitt, Gertrud | |
dc.contributor.author | Speicher-Mentges, Susanne | |
dc.contributor.author | Cai, Xiaoyu | |
dc.contributor.author | Meng, Weikun | |
dc.contributor.author | Cucchiarini, Magali | |
dc.contributor.author | Maihöfer, Johanna | |
dc.contributor.author | Goebel, Lars | |
dc.contributor.author | Zurakowski, David | |
dc.contributor.author | Menger, Michael D. | |
dc.contributor.author | Laschke, Matthias W. | |
dc.date.accessioned | 2024-07-09T17:35:20Z | |
dc.date.available | 2024-07-09T17:35:20Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | J. Maihöfer, H. Madry, A. Rey-Rico, J. K. Venkatesan, L. Goebel, G. Schmitt, S. Speicher-Mentges, X. Cai, W. Meng, D. Zurakowski, M. D. Menger, M. W. Laschke, M. Cucchiarini, Hydrogel-Guided, rAAV-Mediated IGF-I Overexpression Enables Long-Term Cartilage Repair and Protection against Perifocal Osteoarthritis in a Large-Animal Full-Thickness Chondral Defect Model at One Year In Vivo. Adv. Mater. 2021, 33, 2008451. https://doi.org/10.1002/adma.202008451 | es_ES |
dc.identifier.uri | http://hdl.handle.net/2183/37847 | |
dc.description.abstract | [Abstract] The regeneration of focal articular cartilage defects is complicated by the reduced quality of the repair tissue and the potential development of perifocal osteoarthritis (OA). Biomaterial-guided gene therapy may enhance cartilage repair by controlling the release of therapeutic sequences in a spatiotemporal manner. Here, the benefits of delivering a recombinant adeno-associated virus (rAAV) vector coding for the human insulin-like growth factor I (IGF-I) via an alginate hydrogel (IGF-I/AlgPH155) to enhance repair of full-thickness chondral defects following microfracture surgery after one year in minipigs versus control (lacZ/AlgPH155) treatment are reported. Sustained IGF-I overexpression is significantly achieved in the repair tissue of defects treated with IGF-I/AlgPH155 versus those receiving lacZ/AlgPH155 for one year and in the cartilage surrounding the defects. Administration of IGF-I/AlgPH155 significantly improves parameters of cartilage repair at one year relative to lacZ/AlgPH155 (semiquantitative total histological score, cell densities, matrix deposition) without deleterious or immune reactions. Remarkably, delivery of IGF-I/AlgPH155 also significantly reduces perifocal OA and inflammation after one year versus lacZ/AlgPH155 treatment. Biomaterial-guided rAAV gene transfer represents a valuable clinical approach to promote cartilage repair and to protect against OA. | es_ES |
dc.description.sponsorship | J.M. and H.M. contributed equally to this work. The authors thankDr. R. J. Samulski (The Gene Therapy Center, University of North Carolina,Chapel Hill, NC, USA), Dr. X. Xiao (The Gene Therapy Center, Universityof Pittsburgh, Pittsburgh, PA, USA), and E. F. Terwilliger (Division ofExperimental Medicine, Harvard Institutes of Medicine and Beth Israel Deaconess Medical Center, Boston, MA, USA) for providing thegenomic AAV-2 plasmid clones and the 293 cell line. This work wassupported by a grant from the Deutsche Arthrose-Hilfe e.V. (M.C.,H.M.) and by the Saarland University within the funding programmeOpen Access Publishing.Open access funding enabled and organized by Projekt DEAL. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley-VCH | es_ES |
dc.relation.uri | https://doi.org/10.1002/adma.202008451 | es_ES |
dc.rights | Creative CommonsAttribution-NonCommercial-NoDerivs License | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Alginate hydrogel | es_ES |
dc.subject | Cartilage repair | es_ES |
dc.subject | IGF-I | es_ES |
dc.subject | Perifocal osteoarthritis | es_ES |
dc.subject | RAAV | es_ES |
dc.title | Hydrogel-Guided, rAAV-Mediated IGF-I Overexpression Enables Long-Term Cartilage Repair and Protection against Perifocal Osteoarthritis in a Large-Animal Full-Thickness Chondral Defect Model at One Year In Vivo | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Advanced Materials | es_ES |
UDC.volume | 33 | es_ES |
UDC.issue | 16 | es_ES |
UDC.startPage | Article 2008451 | es_ES |
dc.identifier.doi | 10.1002/adma.202008451 |