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In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii
dc.contributor.author | Conde-Pérez, Kelly | |
dc.contributor.author | Vázquez-Ucha, Juan Carlos | |
dc.contributor.author | Álvarez-Fraga, Laura | |
dc.contributor.author | Ageitos, Lucía | |
dc.contributor.author | Rumbo-Feal, Soraya | |
dc.contributor.author | Martínez-Guitián, Marta | |
dc.contributor.author | Trigo Tasende, Noelia | |
dc.contributor.author | Rodríguez, Jaime | |
dc.contributor.author | Bou, Germán | |
dc.contributor.author | Jiménez, Carlos | |
dc.contributor.author | Beceiro, Alejandro | |
dc.contributor.author | Poza, Margarita | |
dc.date.accessioned | 2022-02-18T09:40:32Z | |
dc.date.available | 2022-02-18T09:40:32Z | |
dc.date.issued | 2021-10-05 | |
dc.identifier.citation | Conde-Pérez K, Vázquez-Ucha JC, Álvarez-Fraga L, Ageitos L, Rumbo-Feal S, Martínez-Guitián M, Trigo-Tasende N, Rodríguez J, Bou G, Jiménez C, Beceiro A and Poza M (2021) In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii. Front. Microbiol. 12:752070. doi: 10.3389/fmicb.2021.752070 | es_ES |
dc.identifier.issn | 1664-302X | |
dc.identifier.uri | http://hdl.handle.net/2183/29813 | |
dc.description.abstract | [Abstract] Acinetobacter baumannii is a multidrug-resistant pathogen that represents a serious threat to global health. A. baumannii possesses a wide range of virulence factors that contribute to the bacterial pathogenicity. Among them, the siderophore acinetobactin is one of the most important, being essential for the development of the infection. In this study we performed an in-depth analysis of the acinetobactin cluster in the strain A. baumannii ATCC 17978. For this purpose, nineteen individual isogenic mutant strains were generated, and further phenotypical analysis were performed. Individual mutants lacking the biosynthetic genes entA, basG, basC, basD, and basB showed a significant loss in virulence, due to the disruption in the acinetobactin production. Similarly, the gene bauA, coding for the acinetobactin receptor, was also found to be crucial for the bacterial pathogenesis. In addition, the analysis of the ΔbasJ/ΔfbsB double mutant strain demonstrated the high level of genetic redundancy between siderophores where the role of specific genes of the acinetobactin cluster can be fulfilled by their fimsbactin redundant genes. Overall, this study highlights the essential role of entA, basG, basC, basD, basB and bauA in the pathogenicity of A. baumannii and provides potential therapeutic targets for the design of new antivirulence agents against this microorganism. | es_ES |
dc.description.sponsorship | This work was funded by Projects PI15/00860 awarded to GB and PI17/01482 to AB and MP, all within in the National Plan for Scientific Research, Development and Technological Innovation 2013–2016 and funded by the ISCIII – General Subdirection of Assessment and Promotion of the Research-European Regional Development Fund (FEDER) “A way of making Europe.” The study was also funded by project IN607A 2016/22 (GAIN- Agencia Gallega de Innovación – Consellería de Economía, Emprego e Industria) awarded to GB. This work was also supported by Planes Nacionales de I + D + i 2008–2011/2013–2016 and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD16/0016/006) co-financed by European Development Regional Fund “A way to achieve Europe” and operative program Intelligent Growth 2014–2020. This work was also supported by Grant RTI2018-093634-B-C22 (AEI/FEDER, EU) from the State Agency for Research (AEI) of Spain, co-funded by the FEDER Programme from the European Union and Xunta de Galicia for the support of Grant ED431E 2018/03 for CICA-INIBIC strategic and the initiative “Seed Projects 2019–2020.” JV-U was financially supported by the ISCIII project FI18/00315, LÁ-F by the ISCIII project PI14/00059 and the IN606B-2018/011, MM-G was financially supported by the Grant Clara Roy (SEIMC, Spanish Society of Clinical Microbiology and Infectious Diseases), KC-P by IN607A 2016/22 and AECC (Asociación Española Contra el Cáncer) predoctoral fellowship and LA by Xunta de Galicia co-funded with the European Social Fund (FSE) of the European Union (ED481A-2019/081) | es_ES |
dc.description.sponsorship | Xunta de Galicia; IN607A 2016/22 | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED431E 2018/03 | es_ES |
dc.description.sponsorship | Xunta de Galicia; IN606B-2018/011 | es_ES |
dc.description.sponsorship | Xunta de Galicia; IN607A 2016/22 | es_ES |
dc.description.sponsorship | Xunta de Galicia; ED481A-2019/081 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI15%2F00860/ES/Desarrollo de una plataforma universal de vacunas bacterianas vivas atenuadas auxótrofas para D-glutamato: prevención y erradicación de infecciones por bacterias multirresistentes/ | |
dc.relation | info:eu-repo/grantAgreement/MICINN/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PI17%2F01482/ES/EVALUACIÓN DE NUEVAS ESTRATEGIAS ANTIMICROBIANAS MEDIANTE SILENCIAMIENTO DE ARN VEHICULIZADO EN NANOCÁPSULAS E INHIBIDORES ENZIMÁTICOS/ | |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RD16%2F0016%2F0006/ES/RED ESPAÑOLA DE INVESTIGACIÓN EN PATOLOGÍAS INFECCIOSAS/ | |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093634-B-C22/ES/FACTORES DE VIRULENCIA BACTERIANOS COMO DIANAS TERAPEUTICAS EN PECES: CARACTERIZACION DE SIDEROFOROS Y DESARROLLO DE NUEVOS TRATAMIENTOS CONTRA FORUNCULOSIS Y TENACIBACULOSIS/ | |
dc.relation | info:eu-repo/grantAgreement/MICINN/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/FI18%2F00315/ES/ | |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PI14%2F00059/ES/Nuevas estrategias frente al patógeno multirresistente Acinetobacter baumannii: silenciamiento (siRNA) bacteriano y nuevos inhibidores químicos. Evaluación en estudios preclínicos/ | |
dc.relation.uri | https://doi.org/10.3389/fmicb.2021.752070 | es_ES |
dc.rights | Atribución 4.0 Internacional | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Acinetobacter baumannii | es_ES |
dc.subject | Acinetobactin | es_ES |
dc.subject | Fimsbactin | es_ES |
dc.subject | Iron uptake | es_ES |
dc.subject | Siderophore | es_ES |
dc.subject | Virulence | es_ES |
dc.subject | Mouse sepsis infection | es_ES |
dc.title | In-Depth Analysis of the Role of the Acinetobactin Cluster in the Virulence of Acinetobacter baumannii | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Frontiers in Microbiology | es_ES |
UDC.volume | 12 | es_ES |
UDC.startPage | 752070 | es_ES |
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