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Mitochondrial dysfunction plays a relevant role in pathophysiology of peritoneal membrane damage induced by peritoneal dialysis
dc.contributor.author | Ramil-Gómez, Olalla | |
dc.contributor.author | Rodríguez-Carmona, Ana | |
dc.contributor.author | Fernández-Rodríguez, Jennifer Adriana | |
dc.contributor.author | Pérez-Fontán, Miguel | |
dc.contributor.author | Ferreiro-Hermida, Tamara | |
dc.contributor.author | López Pardo, Mirian | |
dc.contributor.author | Pérez-López, Teresa | |
dc.contributor.author | López-Armada, María José | |
dc.date.accessioned | 2021-03-30T12:09:29Z | |
dc.date.available | 2021-03-30T12:09:29Z | |
dc.date.issued | 2021-03-13 | |
dc.identifier.citation | Ramil-Gómez O, Rodríguez-Carmona A, Fernández-Rodríguez JA, Pérez-Fontán M, Ferreiro-Hermida T, López-Pardo M, Pérez-López T, López-Armada MJ. Mitochondrial dysfunction plays a relevant role in pathophysiology of peritoneal membrane damage induced by peritoneal dialysis. Antioxidants (Basel). 2021 Mar 13;10(3):447. | es_ES |
dc.identifier.issn | 2076-3921 | |
dc.identifier.uri | http://hdl.handle.net/2183/27648 | |
dc.description.abstract | [Abstract] Preservation of the peritoneal membrane is an essential determinant of the long-term outcome of peritoneal dialysis (PD). Epithelial-to-mesenchymal transition (EMT) plays a central role in the pathogenesis of PD-related peritoneal membrane injury. We hypothesized that mitochondria may be implicated in the mechanisms that initiate and sustain peritoneal membrane damage in this setting. Hence, we carried out ex vivo studies of effluent-derived human mesothelial cells, which disclosed a significant increase in mitochondrial reactive oxygen species (mtROS) production and a loss of mitochondrial membrane potential in mesothelial cells with a fibroblast phenotype, compared to those preserving an epithelial morphology. In addition, in vitro studies of omentum-derived mesothelial cells identified mtROS as mediators of the EMT process as mitoTEMPO, a selective mtROS scavenger, reduced fibronectin protein expression induced by TGF-ß1. Moreover, we quantified mitochondrial DNA (mtDNA) levels in the supernatant of effluent PD solutions, disclosing a direct correlation with small solute transport characteristics (as estimated from the ratio dialysate/plasma of creatinine at 240 min), and an inverse correlation with peritoneal ultrafiltration. These results suggest that mitochondria are involved in the EMT that human peritoneal mesothelial cells suffer in the course of PD therapy. The level of mtDNA in the effluent dialysate of PD patients could perform as a biomarker of PD-induced damage to the peritoneal membrane. | es_ES |
dc.description.sponsorship | This research was funded by Fondo de Investigación Sanitaria (PI15/02218 and PI18/01803); and AGRUP2015/05 and AGRUP2018/03, CICA-INIBIC) integrated in the National Plan for Scientific Program, Development and Technological Innovation 2013–2016 and funded by the ISCIII-General Subdirection of Assessment and Promotion of Research—European Regional Development Fund (FEDER) “A way of making Europe”. O.R.-G. was supported by a grant from Diputación A Coruña 2015, a grant from Fundación Novoa Santos 2018 and a Contrato predoctoral ISCIII (PI18/01803), and J.A.F.-R. is recipient of a grant from Diputación A Coruña 2018. | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/PI15%2F02218/ES/Papel de la disfunción mitocondrial en los cambios estructurales y funcionales de la membrana peritoneal en pacientes tratados con diálisis peritoneal. Efecto de una dieta antioxidante | es_ES |
dc.description.sponsorship | info:eu-repo/grantAgreement/ISCIII/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/PI18%2F01803/ES/PAPEL DE LA AUTOFAGIA EN EL REMODELADO DE LA MEMBRANA PERITONEAL ASOCIADO A PACIENTES TRATADOS CON DIALISIS PERITONEAL. EFECTO DE UNA DIETA ANTIOXIDANTE | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.relation.uri | https://doi.org/10.3390/antiox10030447 | es_ES |
dc.rights | Creative Commons Attribution 4.0 International License (CC-BY 4.0) | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Peritoneal dialysis | es_ES |
dc.subject | Epithelial-to-mesenchymal transition | es_ES |
dc.subject | Oxidative stress | es_ES |
dc.subject | Mitochondria | es_ES |
dc.subject | Mitochondrial DNA | es_ES |
dc.subject | Biomarker | es_ES |
dc.subject | Mesothelial cells | es_ES |
dc.title | Mitochondrial dysfunction plays a relevant role in pathophysiology of peritoneal membrane damage induced by peritoneal dialysis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.access | info:eu-repo/semantics/openAccess | es_ES |
UDC.journalTitle | Antioxidants | es_ES |
UDC.volume | 10 | es_ES |
UDC.issue | 3 | es_ES |
UDC.startPage | 447 | es_ES |