Automatic Detection of Freshwater Phytoplankton Specimens in Conventional Microscopy Images
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Automatic Detection of Freshwater Phytoplankton Specimens in Conventional Microscopy ImagesDate
2020-11-23Citation
Rivas-Villar, D.; Rouco, J.; Penedo, M.G.; Carballeira, R.; Novo, J. Automatic Detection of Freshwater Phytoplankton Specimens in Conventional Microscopy Images. Sensors 2020, 20, 6704. https://doi.org/10.3390/s20226704
Abstract
[Abstract]
Water safety and quality can be compromised by the proliferation of toxin-producing phytoplankton species, requiring continuous monitoring of water sources. This analysis involves the identification and counting of these species which requires broad experience and knowledge. The automatization of these tasks is highly desirable as it would release the experts from tedious work, eliminate subjective factors, and improve repeatability. Thus, in this preliminary work, we propose to advance towards an automatic methodology for phytoplankton analysis in digital images of water samples acquired using regular microscopes. In particular, we propose a novel and fully automatic method to detect and segment the existent phytoplankton specimens in these images using classical computer vision algorithms. The proposed method is able to correctly detect sparse colonies as single phytoplankton candidates, thanks to a novel fusion algorithm, and is able to differentiate phytoplankton specimens from other image objects in the microscope samples (like minerals, bubbles or detritus) using a machine learning based approach that exploits texture and colour features. Our preliminary experiments demonstrate that the proposed method provides satisfactory and accurate results.
Keywords
Microscope images
Phytoplankton detection
Colony merging
Gabor filters
Bag of visual words
Phytoplankton detection
Colony merging
Gabor filters
Bag of visual words
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Rights
Atribución 4.0 Internacional
ISSN
1424-8220