A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues
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A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissuesAutor(es)
Fecha
2011-05-18Cita bibliográfica
Rodríguez-Rigueiro T, Valladares-Ayerbes M, Haz-Conde M, et al. A novel procedure for protein extraction from formalin-fixed paraffin-embedded tissues. Proteomics. 2011;11(12): 2555-2559
Resumen
[Abstract] Most of the archived pathological specimens in hospitals are kept as formalin-fixed paraffin-embedded tissues (FFPE) for long-term preservation. Up to now, these samples are only used for immunohistochemistry in a clinical routine as it is difficult to recover intact protein from these FFPE tissues. Here, we report a novel, short time-consuming and cost-effective method to extract full-length, non-degraded proteins from FFPE tissues. This procedure is combined with an effective and non-toxic deparaffinisation process and an extraction method based on antigen-retrieval, high concentration of SDS and high temperature. We have obtained enough intact protein to be detected by Western blotting analysis. This technique will allow utilising these stored FFPE tissues in several applications for protein analysis helping to advance the translational studies in cancer and other diseases.
Palabras clave
Biomedicine
Cancer
FFPE
Human samples
Protein expression
Western blotting
Cancer
FFPE
Human samples
Protein expression
Western blotting
Versión del editor
Derechos
This is the peer reviewed version of the article which has been published in final form at Wiley Online Library. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving.
ISSN
1615-9853
1615-9861
1615-9861